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The map label for this gene is dnaN [H]

Identifier: 154243960

GI number: 154243960

Start: 2273

End: 3394

Strand: Direct

Name: dnaN [H]

Synonym: Xaut_0002

Alternate gene names: 154243960

Gene position: 2273-3394 (Clockwise)

Preceding gene: 154243959

Following gene: 154243961

Centisome position: 0.04

GC content: 66.93

Gene sequence:

>1122_bases
ATGAAGGTCACCGTCGAGCGCGCCGAGCTTCTCAAATCGCTCAGCCACGTCCACCGCGTCGTCGAGCGGCGCAACACCAT
TCCGATTCTCGCCAACGTGCTGATCAAGGCGGGCAAGAACGGGCTGGAACTGAAAGCCACCGACCTCGACCTGGAGGTGG
TGGAGAGCGTTCCGGCCGAGACCGGCCAGGCCGGGGCCACCACCGTGCCCGCCCATGTGCTCTACGACATCGTGCGCAAA
CTGCCCGAGGGTGCGCAGGTGCAACTGGAGGCGGGCGGCGAGCGGGGCACCCTCACCGTCAAGGCGTCCCGCGCCCGGTT
CTCGCTGCAGACCCTGCCCGAGGCCGACTTCCCGGACCTCGCGGCGGGCGAATTCTCCCATACCTTCAAGGTGAAGGGCG
CGGACTTCCGGCGCCTGGTGGACAAGACCCAGTTCGCCATCTCCACCGAGGAGACGCGCTATTACCTGAACGGCATCTAT
CTCCACGTCACCGAGGGCGCCGGCGGCAAGTCCATGCTGCGGGCGGTGGCCACCGACGGCCACCGCCTCGCCCAGGCCGA
GCTGGAGGCGCCGGCGGGCGCGGCCGGCCTGCCCGGCGTCATCGTGCCGCGCAAGACCGTGGCCGAGATCCAAAAGCTGC
TGGAGGACAAGGAGGCGGAGGCTACCGTCTCGCTCTCCACCACCAAGATCCGGGTGCAGGTGGGCTCCATCGTGCTCACC
TCCAAGCTCATCGACGGCACCTTCCCTGATTATGGAAGGGTTATTCCCCAGGGCAACGACAAGACCCTGATCGTGGACAA
GGACGAGTTCGCCGCCGCGGTGGACCGCGTCTCCACAGTGTCCAGCGAGCGCGGGCGGGCGGTGAAGCTGTCGCTGGCCG
AGGGCAAGATGACACTCTCCGTCACCAATCCCGATTCGGGCAGCGCCACCGAGGAACTGGAGGTGGACTACACCGCCGAG
CCCCTCGATATCGGCTTCAACTCCCGCTACCTGCTGGACATCACCTCGCAGCTGGAAGGCGACACGGCTGAACTGAAGCT
GGCCGACCCCGGCTCGCCCACCCTGGTGCGCGATTCCACCAAGACGGATGCGCTCTACGTCCTCATGCCCATGCGGGTGT
GA

Upstream 100 bases:

>100_bases
GGAACGGGCGCCGTCCCCGAACGACGAGGCGGACCCACCGGCGGGACGTAGGCGCCCGTGGGAGGCCGGCTCCAGAGCGA
AGAGTGAACGGGCCAGGACC

Downstream 100 bases:

>100_bases
TCGCAAAGGCCGGTGAGCGCCGCTCGCGGGCCCTTGGTCCAGCCCGCGCGGCGGCCTGCTGGGCGATACTCTTCCGGCGC
CGGTCGAAGACCGGGGCCGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIKAGKNGLELKATDLDLEVVESVPAETGQAGATTVPAHVLYDIVRK
LPEGAQVQLEAGGERGTLTVKASRARFSLQTLPEADFPDLAAGEFSHTFKVKGADFRRLVDKTQFAISTEETRYYLNGIY
LHVTEGAGGKSMLRAVATDGHRLAQAELEAPAGAAGLPGVIVPRKTVAEIQKLLEDKEAEATVSLSTTKIRVQVGSIVLT
SKLIDGTFPDYGRVIPQGNDKTLIVDKDEFAAAVDRVSTVSSERGRAVKLSLAEGKMTLSVTNPDSGSATEELEVDYTAE
PLDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTKTDALYVLMPMRV

Sequences:

>Translated_373_residues
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIKAGKNGLELKATDLDLEVVESVPAETGQAGATTVPAHVLYDIVRK
LPEGAQVQLEAGGERGTLTVKASRARFSLQTLPEADFPDLAAGEFSHTFKVKGADFRRLVDKTQFAISTEETRYYLNGIY
LHVTEGAGGKSMLRAVATDGHRLAQAELEAPAGAAGLPGVIVPRKTVAEIQKLLEDKEAEATVSLSTTKIRVQVGSIVLT
SKLIDGTFPDYGRVIPQGNDKTLIVDKDEFAAAVDRVSTVSSERGRAVKLSLAEGKMTLSVTNPDSGSATEELEVDYTAE
PLDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTKTDALYVLMPMRV
>Mature_373_residues
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIKAGKNGLELKATDLDLEVVESVPAETGQAGATTVPAHVLYDIVRK
LPEGAQVQLEAGGERGTLTVKASRARFSLQTLPEADFPDLAAGEFSHTFKVKGADFRRLVDKTQFAISTEETRYYLNGIY
LHVTEGAGGKSMLRAVATDGHRLAQAELEAPAGAAGLPGVIVPRKTVAEIQKLLEDKEAEATVSLSTTKIRVQVGSIVLT
SKLIDGTFPDYGRVIPQGNDKTLIVDKDEFAAAVDRVSTVSSERGRAVKLSLAEGKMTLSVTNPDSGSATEELEVDYTAE
PLDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDSTKTDALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=41.2234042553192, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40164; Mature: 40164

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIKAGKNGLELKATDLDLEVVESVPAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCC
TGQAGATTVPAHVLYDIVRKLPEGAQVQLEAGGERGTLTVKASRARFSLQTLPEADFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCC
AAGEFSHTFKVKGADFRRLVDKTQFAISTEETRYYLNGIYLHVTEGAGGKSMLRAVATDG
CCCCCCEEEEECCCHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCHHHHHHHHHCCC
HRLAQAELEAPAGAAGLPGVIVPRKTVAEIQKLLEDKEAEATVSLSTTKIRVQVGSIVLT
CCEEHHHHCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCEEEE
SKLIDGTFPDYGRVIPQGNDKTLIVDKDEFAAAVDRVSTVSSERGRAVKLSLAEGKMTLS
EHHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEE
VTNPDSGSATEELEVDYTAEPLDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDST
EECCCCCCCCCEEEEEECCCCEECCCCCEEEEEEHHHCCCCCEEEEEECCCCCCEEECCC
KTDALYVLMPMRV
CCCEEEEEEECCC
>Mature Secondary Structure
MKVTVERAELLKSLSHVHRVVERRNTIPILANVLIKAGKNGLELKATDLDLEVVESVPAE
CEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCC
TGQAGATTVPAHVLYDIVRKLPEGAQVQLEAGGERGTLTVKASRARFSLQTLPEADFPDL
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCEEEEECCCCCCCCCC
AAGEFSHTFKVKGADFRRLVDKTQFAISTEETRYYLNGIYLHVTEGAGGKSMLRAVATDG
CCCCCCEEEEECCCHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCHHHHHHHHHCCC
HRLAQAELEAPAGAAGLPGVIVPRKTVAEIQKLLEDKEAEATVSLSTTKIRVQVGSIVLT
CCEEHHHHCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCEEEE
SKLIDGTFPDYGRVIPQGNDKTLIVDKDEFAAAVDRVSTVSSERGRAVKLSLAEGKMTLS
EHHHCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEE
VTNPDSGSATEELEVDYTAEPLDIGFNSRYLLDITSQLEGDTAELKLADPGSPTLVRDST
EECCCCCCCCCEEEEEECCCCEECCCCCEEEEEEHHHCCCCCEEEEEECCCCCCEEECCC
KTDALYVLMPMRV
CCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]