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The map label for this gene is ansA [H]

Identifier: 153951810

GI number: 153951810

Start: 37681

End: 38727

Strand: Direct

Name: ansA [H]

Synonym: JJD26997_0031

Alternate gene names: 153951810

Gene position: 37681-38727 (Clockwise)

Preceding gene: 153952383

Following gene: 153951062

Centisome position: 2.04

GC content: 35.05

Gene sequence:

>1047_bases
GTGAAAAAGTTACTATTATGTGTATTAGTATTTTTATCTATAGGAGCATGTATGGCAGAAGCTAAACCAAAGATTGCTAT
TTTAGCAACAGGTGGAACGATAGCAGGTTCTATTGATAGCGCTGTTGCCACAACAGGTTATACAGCCGGAGTTGTTGGCG
TAGATGTTTTAATCAAAGCAGTTCCACAAATTCAAGATTTAGCTAATATTAGCGGAGAGCAAATAGCAAATATCGATAGT
TCAAATATGCGTGATGAAATTTGGCTAAAGCTTGCCAAAGAAATCAATAAGCTTTTTGCCGAAGGGGTAGATGGTGTGGT
TATAACTCATGGTACTGACACCATGGAAGAAACTGTTTATTTTTTAAATTTAACCATTAAAAGTGATAAGCCTGTAGTTT
TAGTTGGCGCTATGCGTCCATCAACTGCTATAAGTGCAGATGGGCCAAAAAATCTTTACAATGCTGTAGCATTAGCTGCT
GACAAAGAATCTAAGGGCAAGGGTGTTATGGTAGCTATGAATGATAAAATTTTAAGCGCTAGAGGCGTGGTAAAAACTCA
TAGTTTAAATGTTGATGCTTTCTCTTCTCCTGATTTTGGGGATTTGGGTTATATAATAGATGGTAAAGTCTTTTTCTATA
ATAATATTGCTAAAGCACACACAAAAAATACTCCTTTTGATGTAAGCAAACTCACAAGTTTGCCAAAAGTAGATATTCTT
TATAGCTATTCAAATGATGGTAGTGGTGTCGCTGCAAAAGCTTTATTTGAACATGGAACTAAGGGTGTAGTTGTTGCAGG
AAGTGGTGCAGGAAGTATACATGAAGATCAAAAAAATGTTTTAAAAGAGCTTATGAAAAAAGGACTTGATGTTGTTGTGA
GCTCAAGGGTTGTGGCAGGACATGTTGCTGTGAGTGATTCAGATAAAAAATTAGGTTTTATTAGTGCTGAAGATTTAAAC
CCTCAAAAAGCAAGAGTTTTGCTTATGTTAGCGCTTACAAAAACAAGTGATCCTAAAAAAATTCAAGAGTATTTTTTAAA
ATATTAA

Upstream 100 bases:

>100_bases
AAAGAAATTTTATAAAATTTATAAAATTTTTATTTATTATTTATTAATATTATATAAGTTTCATTAATGAAACGAATTTT
AACAAAATAAGGGGAGAATT

Downstream 100 bases:

>100_bases
AATTTTTAAATATTTCTAAACTTAGGGAATTTATCTTCTCTAAGTCAAGCCTTTCAATGTTATATTCGTATATTATATCT
TATATTCTTTTATTATCATT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 2; L-asparagine amidohydrolase 2 [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKLLLCVLVFLSIGACMAEAKPKIAILATGGTIAGSIDSAVATTGYTAGVVGVDVLIKAVPQIQDLANISGEQIANIDS
SNMRDEIWLKLAKEINKLFAEGVDGVVITHGTDTMEETVYFLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALAA
DKESKGKGVMVAMNDKILSARGVVKTHSLNVDAFSSPDFGDLGYIIDGKVFFYNNIAKAHTKNTPFDVSKLTSLPKVDIL
YSYSNDGSGVAAKALFEHGTKGVVVAGSGAGSIHEDQKNVLKELMKKGLDVVVSSRVVAGHVAVSDSDKKLGFISAEDLN
PQKARVLLMLALTKTSDPKKIQEYFLKY

Sequences:

>Translated_348_residues
MKKLLLCVLVFLSIGACMAEAKPKIAILATGGTIAGSIDSAVATTGYTAGVVGVDVLIKAVPQIQDLANISGEQIANIDS
SNMRDEIWLKLAKEINKLFAEGVDGVVITHGTDTMEETVYFLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALAA
DKESKGKGVMVAMNDKILSARGVVKTHSLNVDAFSSPDFGDLGYIIDGKVFFYNNIAKAHTKNTPFDVSKLTSLPKVDIL
YSYSNDGSGVAAKALFEHGTKGVVVAGSGAGSIHEDQKNVLKELMKKGLDVVVSSRVVAGHVAVSDSDKKLGFISAEDLN
PQKARVLLMLALTKTSDPKKIQEYFLKY
>Mature_348_residues
MKKLLLCVLVFLSIGACMAEAKPKIAILATGGTIAGSIDSAVATTGYTAGVVGVDVLIKAVPQIQDLANISGEQIANIDS
SNMRDEIWLKLAKEINKLFAEGVDGVVITHGTDTMEETVYFLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALAA
DKESKGKGVMVAMNDKILSARGVVKTHSLNVDAFSSPDFGDLGYIIDGKVFFYNNIAKAHTKNTPFDVSKLTSLPKVDIL
YSYSNDGSGVAAKALFEHGTKGVVVAGSGAGSIHEDQKNVLKELMKKGLDVVVSSRVVAGHVAVSDSDKKLGFISAEDLN
PQKARVLLMLALTKTSDPKKIQEYFLKY

Specific function: Catalyzes the conversion of L-asparagine to L-aspartate and ammonium [H]

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=351, Percent_Identity=53.8461538461538, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI1788065, Length=278, Percent_Identity=25.8992805755396, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=360, Percent_Identity=25.5555555555556, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17558278, Length=360, Percent_Identity=25.5555555555556, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17558280, Length=360, Percent_Identity=25.5555555555556, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI193207374, Length=360, Percent_Identity=25.5555555555556, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=357, Percent_Identity=39.2156862745098, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323187, Length=357, Percent_Identity=39.2156862745098, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323186, Length=357, Percent_Identity=39.2156862745098, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6323184, Length=357, Percent_Identity=39.2156862745098, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6320527, Length=338, Percent_Identity=36.094674556213, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24645530, Length=272, Percent_Identity=30.5147058823529, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24639609, Length=262, Percent_Identity=26.3358778625954, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36922; Mature: 36922

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLCVLVFLSIGACMAEAKPKIAILATGGTIAGSIDSAVATTGYTAGVVGVDVLIKA
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCHHHHHCCCCCHHHHHHHHHHH
VPQIQDLANISGEQIANIDSSNMRDEIWLKLAKEINKLFAEGVDGVVITHGTDTMEETVY
HHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHEEE
FLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALAADKESKGKGVMVAMNDKILSA
EEEEEEECCCCEEEEECCCCCCEECCCCCHHHHHHHEEEECCCCCCCEEEEEECCCEECC
RGVVKTHSLNVDAFSSPDFGDLGYIIDGKVFFYNNIAKAHTKNTPFDVSKLTSLPKVDIL
CCEEEEEEECCCCCCCCCCCCCCEEEECEEEEEECHHHHCCCCCCCCHHHHCCCCCEEEE
YSYSNDGSGVAAKALFEHGTKGVVVAGSGAGSIHEDQKNVLKELMKKGLDVVVSSRVVAG
EEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEE
HVAVSDSDKKLGFISAEDLNPQKARVLLMLALTKTSDPKKIQEYFLKY
EEEECCCCCCEEEEECCCCCHHHHEEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKLLLCVLVFLSIGACMAEAKPKIAILATGGTIAGSIDSAVATTGYTAGVVGVDVLIKA
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCHHHHHCCCCCHHHHHHHHHHH
VPQIQDLANISGEQIANIDSSNMRDEIWLKLAKEINKLFAEGVDGVVITHGTDTMEETVY
HHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHEEE
FLNLTIKSDKPVVLVGAMRPSTAISADGPKNLYNAVALAADKESKGKGVMVAMNDKILSA
EEEEEEECCCCEEEEECCCCCCEECCCCCHHHHHHHEEEECCCCCCCEEEEEECCCEECC
RGVVKTHSLNVDAFSSPDFGDLGYIIDGKVFFYNNIAKAHTKNTPFDVSKLTSLPKVDIL
CCEEEEEEECCCCCCCCCCCCCCEEEECEEEEEECHHHHCCCCCCCCHHHHCCCCCEEEE
YSYSNDGSGVAAKALFEHGTKGVVVAGSGAGSIHEDQKNVLKELMKKGLDVVVSSRVVAG
EEECCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEE
HVAVSDSDKKLGFISAEDLNPQKARVLLMLALTKTSDPKKIQEYFLKY
EEEECCCCCCEEEEECCCCCHHHHEEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]