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The map label for this gene is miaA [H]

Identifier: 153951773

GI number: 153951773

Start: 169775

End: 170644

Strand: Direct

Name: miaA [H]

Synonym: JJD26997_0181

Alternate gene names: 153951773

Gene position: 169775-170644 (Clockwise)

Preceding gene: 153952354

Following gene: 153951398

Centisome position: 9.2

GC content: 27.47

Gene sequence:

>870_bases
ATGTTTTTTGAAATAGCATTAATAGGAACAACCGCGAGTGGAAAAACATACATAGCTGATACTTTAGCAAGAGAATTTGA
TGCTGTTGTTTTAAGTCTTGATAGTCTTTGTGTTTATAAAGAGATTAATATAGCAAGCGCCAAGCCTTCACAAGATGATC
TTGCGAGTATAAAATACTTTGGGGTAAATTTATTGAGCGTGAATGAACATTTTAACGTTGAGTTATTTATAAGAGAATAC
CAAAAAGCAAAAGAATTTGCTTTAGCTAGAAATTTGCCTCTAATTATAGTAGGTGGAACTGGATTTTATTTAAAAACTAT
GATAGATGGTTTAAGTGAAAAAACCTTAGAGTCAAAGAGTTCTTTAAATAATGATGAAATTTATACTCTTTTATTAAATA
TTGATCCAAACTATAAAATAGAAAAAAATGATACTTATAGATTAAAAAAATGGCTGGGTATTTATGAACAAACAAGAGAA
ATTCCTAGTGAATTTTTAAAAAGAACGCAAAAAACTGGGGTGCTTAAAGATATAGAAATTTATGAGCTTGCGTGGGATAA
AGAAATTTTAAAAAAACGCATTCAAACTAGAACAAAAGAAATGCTTGATAATGGACTTTTAGATGAAGCAAAAATTCTTT
TTTATAAATTTGATCATAAACTTAAAGCGTTAAATTCTATAGGTTTGAAAGAGTGTAAAGAATATTTAGATGGAGAAATT
TCCTTTAAAGATCTTGAAAATTTAATCACGATACATACTACGCAACTTGCCAAAAGACAAAGAACTTTTAATAAAAAATT
TCAAAGTAAAGCCTTGGAATTTGATAAAGCTTTAGCTACTTTAAGAATGAAATTTTCAATCGAAAAATAA

Upstream 100 bases:

>100_bases
ATCTTTAAATTTTATCCAGAATGCGTTCATTTTTCTACTTTAAAATATTTTTTGCTATGATTTTACTAAAATTTTTATAA
ATTTTAGGATAAATATTAAA

Downstream 100 bases:

>100_bases
ATGTTCTATTAGAATTTTTACTCCTAATATAATTAAAACTAATCCACCTAAAAGTTCAGCTTTATTTTTAAGATAAATTC
CAAATTTATTTCCTATTTTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MFFEIALIGTTASGKTYIADTLAREFDAVVLSLDSLCVYKEINIASAKPSQDDLASIKYFGVNLLSVNEHFNVELFIREY
QKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKSSLNNDEIYTLLLNIDPNYKIEKNDTYRLKKWLGIYEQTRE
IPSEFLKRTQKTGVLKDIEIYELAWDKEILKKRIQTRTKEMLDNGLLDEAKILFYKFDHKLKALNSIGLKECKEYLDGEI
SFKDLENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK

Sequences:

>Translated_289_residues
MFFEIALIGTTASGKTYIADTLAREFDAVVLSLDSLCVYKEINIASAKPSQDDLASIKYFGVNLLSVNEHFNVELFIREY
QKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKSSLNNDEIYTLLLNIDPNYKIEKNDTYRLKKWLGIYEQTRE
IPSEFLKRTQKTGVLKDIEIYELAWDKEILKKRIQTRTKEMLDNGLLDEAKILFYKFDHKLKALNSIGLKECKEYLDGEI
SFKDLENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK
>Mature_289_residues
MFFEIALIGTTASGKTYIADTLAREFDAVVLSLDSLCVYKEINIASAKPSQDDLASIKYFGVNLLSVNEHFNVELFIREY
QKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKSSLNNDEIYTLLLNIDPNYKIEKNDTYRLKKWLGIYEQTRE
IPSEFLKRTQKTGVLKDIEIYELAWDKEILKKRIQTRTKEMLDNGLLDEAKILFYKFDHKLKALNSIGLKECKEYLDGEI
SFKDLENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=267, Percent_Identity=23.9700374531835, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790613, Length=283, Percent_Identity=27.9151943462898, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144712, Length=329, Percent_Identity=24.6200607902736, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=30.2083333333333, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24649830, Length=265, Percent_Identity=26.7924528301887, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33431; Mature: 33431

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFEIALIGTTASGKTYIADTLAREFDAVVLSLDSLCVYKEINIASAKPSQDDLASIKYF
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHH
GVNLLSVNEHFNVELFIREYQKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKS
CEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
SLNNDEIYTLLLNIDPNYKIEKNDTYRLKKWLGIYEQTREIPSEFLKRTQKTGVLKDIEI
CCCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
YELAWDKEILKKRIQTRTKEMLDNGLLDEAKILFYKFDHKLKALNSIGLKECKEYLDGEI
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHCCCC
SFKDLENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MFFEIALIGTTASGKTYIADTLAREFDAVVLSLDSLCVYKEINIASAKPSQDDLASIKYF
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHH
GVNLLSVNEHFNVELFIREYQKAKEFALARNLPLIIVGGTGFYLKTMIDGLSEKTLESKS
CEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
SLNNDEIYTLLLNIDPNYKIEKNDTYRLKKWLGIYEQTREIPSEFLKRTQKTGVLKDIEI
CCCCCEEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
YELAWDKEILKKRIQTRTKEMLDNGLLDEAKILFYKFDHKLKALNSIGLKECKEYLDGEI
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHCCCC
SFKDLENLITIHTTQLAKRQRTFNKKFQSKALEFDKALATLRMKFSIEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA