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The map label for this gene is kipI [H]

Identifier: 153951517

GI number: 153951517

Start: 1675553

End: 1676293

Strand: Direct

Name: kipI [H]

Synonym: JJD26997_1900

Alternate gene names: 153951517

Gene position: 1675553-1676293 (Clockwise)

Preceding gene: 153952535

Following gene: 153952182

Centisome position: 90.81

GC content: 31.44

Gene sequence:

>741_bases
ATGTTTAGTGTGCATTTTAGTGGAAGTAAAGCTTTGCTTTTGCGTTTTAAGCAAGAAATTTCACCTCAAATTAATGCTTA
TGTTTTAAGTACGGAACAAAGGATACAAAAAGCCCTAAAAGAAGGTGAAATTTATGGTATTGATGAGTTGGTAAGTGCTT
ATGCAAGTTTGTTAATATACTTTAATCCTTGCGTGCTTTCTTTAAATTCTTTGCTTGATTTTTTAGAAAAAATTAAAAAA
GATATTAAACTTACAAAGCAAAACTCAAGTCTTTGTATAGAAGTTCCACTTTGTTATGATGAAGAATTTGGGCTGGATTT
AGAATTTGTGTGCAAACACAATCAAATTTCAAAAGAAGAACTTATATCTCTTCACACTAAGCCTTATTATCTTGTTTTTA
TGTTAGGTTTCATGGCAGGTTTTCCTTATCTTGGAGGTTTAGATGAAAGACTTTTTACCCCACGCCTTTCAAGCCCTAGA
GCAAAAATAGAAGCTGGAAGTGTAGGTATAGCCGATAAACAAACAGGAGTTTATCCTATATCAAGCCCAGGGGGTTGGCA
AATCATTGCTAGAACACCTTTGGAATTTTTTGATAAAGAAGATGAAAAAAATCCTACTCTTTTAAAAGCGGGTATGTTTT
TAAAATTTAAAGCCATTTCAAAAGATGAATTTTTCAAGATCCAAGAACAAGTTGCTAAAAAAGTCTATCAAAAGGAAATT
TATGAGTATAAAAATCATTGA

Upstream 100 bases:

>100_bases
TAATGTAAAAGCTTTAGAATTTGTTAAAAAAATCAAAGAAAATTTAGAAAAAGAGCAAATTCAAATTTGTGCTTTGGAAA
ATTTTATATAGGTTTTAGCA

Downstream 100 bases:

>100_bases
AGCATCTATAAATTCAAGTTTGCAAGACTTTGGTAGAAAAAAATTTGCTAAATTTGGCATAGCAAGAAGTGGGGCTATGG
ATGAAGATGCCTTAAGAATG

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFSVHFSGSKALLLRFKQEISPQINAYVLSTEQRIQKALKEGEIYGIDELVSAYASLLIYFNPCVLSLNSLLDFLEKIKK
DIKLTKQNSSLCIEVPLCYDEEFGLDLEFVCKHNQISKEELISLHTKPYYLVFMLGFMAGFPYLGGLDERLFTPRLSSPR
AKIEAGSVGIADKQTGVYPISSPGGWQIIARTPLEFFDKEDEKNPTLLKAGMFLKFKAISKDEFFKIQEQVAKKVYQKEI
YEYKNH

Sequences:

>Translated_246_residues
MFSVHFSGSKALLLRFKQEISPQINAYVLSTEQRIQKALKEGEIYGIDELVSAYASLLIYFNPCVLSLNSLLDFLEKIKK
DIKLTKQNSSLCIEVPLCYDEEFGLDLEFVCKHNQISKEELISLHTKPYYLVFMLGFMAGFPYLGGLDERLFTPRLSSPR
AKIEAGSVGIADKQTGVYPISSPGGWQIIARTPLEFFDKEDEKNPTLLKAGMFLKFKAISKDEFFKIQEQVAKKVYQKEI
YEYKNH
>Mature_246_residues
MFSVHFSGSKALLLRFKQEISPQINAYVLSTEQRIQKALKEGEIYGIDELVSAYASLLIYFNPCVLSLNSLLDFLEKIKK
DIKLTKQNSSLCIEVPLCYDEEFGLDLEFVCKHNQISKEELISLHTKPYYLVFMLGFMAGFPYLGGLDERLFTPRLSSPR
AKIEAGSVGIADKQTGVYPISSPGGWQIIARTPLEFFDKEDEKNPTLLKAGMFLKFKAISKDEFFKIQEQVAKKVYQKEI
YEYKNH

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=211, Percent_Identity=34.5971563981043, Blast_Score=127, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 28151; Mature: 28151

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVHFSGSKALLLRFKQEISPQINAYVLSTEQRIQKALKEGEIYGIDELVSAYASLLIY
CEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCCEECHHHHHHHHHHHHHH
FNPCVLSLNSLLDFLEKIKKDIKLTKQNSSLCIEVPLCYDEEFGLDLEFVCKHNQISKEE
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCCCCEEEECCCCCCHHH
LISLHTKPYYLVFMLGFMAGFPYLGGLDERLFTPRLSSPRAKIEAGSVGIADKQTGVYPI
HHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCEEEC
SSPGGWQIIARTPLEFFDKEDEKNPTLLKAGMFLKFKAISKDEFFKIQEQVAKKVYQKEI
CCCCCCEEEEECCHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
YEYKNH
HHHCCC
>Mature Secondary Structure
MFSVHFSGSKALLLRFKQEISPQINAYVLSTEQRIQKALKEGEIYGIDELVSAYASLLIY
CEEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCCEECHHHHHHHHHHHHHH
FNPCVLSLNSLLDFLEKIKKDIKLTKQNSSLCIEVPLCYDEEFGLDLEFVCKHNQISKEE
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEECCCCCCCCEEEECCCCCCHHH
LISLHTKPYYLVFMLGFMAGFPYLGGLDERLFTPRLSSPRAKIEAGSVGIADKQTGVYPI
HHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCEEEC
SSPGGWQIIARTPLEFFDKEDEKNPTLLKAGMFLKFKAISKDEFFKIQEQVAKKVYQKEI
CCCCCCEEEEECCHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
YEYKNH
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]