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The map label for this gene is vibE [H]

Identifier: 153951487

GI number: 153951487

Start: 1264169

End: 1265518

Strand: Direct

Name: vibE [H]

Synonym: JJD26997_1426

Alternate gene names: 153951487

Gene position: 1264169-1265518 (Clockwise)

Preceding gene: 153951125

Following gene: 153952295

Centisome position: 68.51

GC content: 28.44

Gene sequence:

>1350_bases
ATGCATAATTTAAATTTTTCTCATCCTTTTTTAAACAAACTTATAGCTTATAAAGATTTAAATTGTTTGATTTATAAAGA
TAAAACTTATTCTTATTTGGAACTTTTACTTGAAGTTAAAAAATATATCAGTCAATTAAATAATTTAAAAGGCGTTATTG
GAATTTTGGGAGATTATAACTTACAAAGCATAGCTTTATTGTTAGCTTGTATAGAAAAAAGATTAATTGTTGCCCCTTTT
TTAAAATTAAAAAATCAGGATTTTAAAGAATTAGAAGATAAAATTTATGAGGGGCAAATAGATTATCTTTATGATGAAGG
CAAATTTATACCCTATCAAAGCAAGCAGTTAAAACACAATCTCATTAATACCCTTCAAAATCATTCATCCAGTGGCATGA
TTCTTTTTTCAAGCGGAAGTACTGGCAAACCTAAAGCCATGGTTCATAATCTTGATGTTATTTTAAATGTTTATCAAGAT
AAAAAACCTAAGGTTATGAATATTGTTTTGTTTTTAATGTTTGATCATATTGGTGGTTTAAATACTTTATTTAATGTTTT
AGGCATGGGTGCTTGCGGTATAGCTTTAGAAGATAGAAAGAATGTTGAAGCTTTAGCACAAAATATACAAAAATACCAAG
TTTCTTTGCTACCTGCAAGTCCAAGTTTGCTAAATCTTATACTAATGAGTGGAGTAAAAGAAAAGTATGATTTAAATTCT
TTAAAACTTATTACATATGGAACAGAAAAAATGAGTGATTCTTTACTCAATCGCTTAAAATTGGAATTTCCTAAGGTTAG
ATTTCATCAAACATTTGGCACAAGTGAAGTTGGTATAGCTCAAACTAAAACTAAAGATAACATGATCAAATTAGAAGGTA
TGGCTTATAAGATTATTAATAATGAGCTTTACTTAAAAAGTAAAACCCAAAGTTTAGGCTATTTAAATGCTGATAATAGT
GTATTTGATGAAGAGGGGTATTTTGCGACAGGAGATTTGGTTGAGCTTTATAAAGAAAATGAAGAAGAATATATAAAAAT
CATAGGAAGAAGTAAAGAGCTTATAAATGTAGGAGGAGAGAAGGTTGTCCCTCAAGAAGTTGAAGGGGTGATTTTAGAAC
TTGACTATATACAAGATTGTTTAGTGTATGCAAAAGCTAATGCTATTACAGGGCAAAGTGTTTGTGTAAAGGTAATTTTA
AAAGCAAATGTGGAATTTACAAACCTTGAACTCAAAAAGCAACTAAGAATGTATTGTAAAGATAAACTGGCTAATTATAA
AATCCCAACTCAAGTTGAGATTGTAGAGAAGTTAGAATTAAGTCAAAGGTTTAAGAAGGTCAGAAAGTAG

Upstream 100 bases:

>100_bases
CGCTTTGGAGAATTTGAAGATGTGTTAAATGTTATTGAGTTTTTTCTTGATGAAAAAAGTGGTTTTATAACCTCACAAGT
AGTTTATTTGGGTGGAATCA

Downstream 100 bases:

>100_bases
ATCTTTCTACTTTCAAAATATAATCTCACTTTCATTAAATTTCATATTTGCTTTTGGTAATGGTGATTTGGTGGTATAAT
AAGCACTTTCTCCTTTATAA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MHNLNFSHPFLNKLIAYKDLNCLIYKDKTYSYLELLLEVKKYISQLNNLKGVIGILGDYNLQSIALLLACIEKRLIVAPF
LKLKNQDFKELEDKIYEGQIDYLYDEGKFIPYQSKQLKHNLINTLQNHSSSGMILFSSGSTGKPKAMVHNLDVILNVYQD
KKPKVMNIVLFLMFDHIGGLNTLFNVLGMGACGIALEDRKNVEALAQNIQKYQVSLLPASPSLLNLILMSGVKEKYDLNS
LKLITYGTEKMSDSLLNRLKLEFPKVRFHQTFGTSEVGIAQTKTKDNMIKLEGMAYKIINNELYLKSKTQSLGYLNADNS
VFDEEGYFATGDLVELYKENEEEYIKIIGRSKELINVGGEKVVPQEVEGVILELDYIQDCLVYAKANAITGQSVCVKVIL
KANVEFTNLELKKQLRMYCKDKLANYKIPTQVEIVEKLELSQRFKKVRK

Sequences:

>Translated_449_residues
MHNLNFSHPFLNKLIAYKDLNCLIYKDKTYSYLELLLEVKKYISQLNNLKGVIGILGDYNLQSIALLLACIEKRLIVAPF
LKLKNQDFKELEDKIYEGQIDYLYDEGKFIPYQSKQLKHNLINTLQNHSSSGMILFSSGSTGKPKAMVHNLDVILNVYQD
KKPKVMNIVLFLMFDHIGGLNTLFNVLGMGACGIALEDRKNVEALAQNIQKYQVSLLPASPSLLNLILMSGVKEKYDLNS
LKLITYGTEKMSDSLLNRLKLEFPKVRFHQTFGTSEVGIAQTKTKDNMIKLEGMAYKIINNELYLKSKTQSLGYLNADNS
VFDEEGYFATGDLVELYKENEEEYIKIIGRSKELINVGGEKVVPQEVEGVILELDYIQDCLVYAKANAITGQSVCVKVIL
KANVEFTNLELKKQLRMYCKDKLANYKIPTQVEIVEKLELSQRFKKVRK
>Mature_449_residues
MHNLNFSHPFLNKLIAYKDLNCLIYKDKTYSYLELLLEVKKYISQLNNLKGVIGILGDYNLQSIALLLACIEKRLIVAPF
LKLKNQDFKELEDKIYEGQIDYLYDEGKFIPYQSKQLKHNLINTLQNHSSSGMILFSSGSTGKPKAMVHNLDVILNVYQD
KKPKVMNIVLFLMFDHIGGLNTLFNVLGMGACGIALEDRKNVEALAQNIQKYQVSLLPASPSLLNLILMSGVKEKYDLNS
LKLITYGTEKMSDSLLNRLKLEFPKVRFHQTFGTSEVGIAQTKTKDNMIKLEGMAYKIINNELYLKSKTQSLGYLNADNS
VFDEEGYFATGDLVELYKENEEEYIKIIGRSKELINVGGEKVVPQEVEGVILELDYIQDCLVYAKANAITGQSVCVKVIL
KANVEFTNLELKKQLRMYCKDKLANYKIPTQVEIVEKLELSQRFKKVRK

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate, preparing that molecule for the final stages of vibriobactin synthesis [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=364, Percent_Identity=23.9010989010989, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI145693145, Length=358, Percent_Identity=26.8156424581006, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786810, Length=267, Percent_Identity=25.4681647940075, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788595, Length=314, Percent_Identity=24.8407643312102, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788107, Length=345, Percent_Identity=22.6086956521739, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141359, Length=487, Percent_Identity=26.2833675564682, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=349, Percent_Identity=26.3610315186246, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=368, Percent_Identity=25.2717391304348, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=339, Percent_Identity=24.7787610619469, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=363, Percent_Identity=25.068870523416, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=316, Percent_Identity=26.5822784810127, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=340, Percent_Identity=22.3529411764706, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=318, Percent_Identity=24.8427672955975, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=330, Percent_Identity=23.3333333333333, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=327, Percent_Identity=24.7706422018349, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=334, Percent_Identity=24.251497005988, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141361, Length=276, Percent_Identity=25, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=355, Percent_Identity=24.7887323943662, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18859661, Length=372, Percent_Identity=23.3870967741935, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355181, Length=306, Percent_Identity=25.1633986928105, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356947, Length=344, Percent_Identity=25.2906976744186, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648260, Length=303, Percent_Identity=24.4224422442244, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 51194; Mature: 51194

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLNFSHPFLNKLIAYKDLNCLIYKDKTYSYLELLLEVKKYISQLNNLKGVIGILGDYN
CCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
LQSIALLLACIEKRLIVAPFLKLKNQDFKELEDKIYEGQIDYLYDEGKFIPYQSKQLKHN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LINTLQNHSSSGMILFSSGSTGKPKAMVHNLDVILNVYQDKKPKVMNIVLFLMFDHIGGL
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
NTLFNVLGMGACGIALEDRKNVEALAQNIQKYQVSLLPASPSLLNLILMSGVKEKYDLNS
HHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCC
LKLITYGTEKMSDSLLNRLKLEFPKVRFHQTFGTSEVGIAQTKTKDNMIKLEGMAYKIIN
EEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCCEEEECCEEEEEEC
NELYLKSKTQSLGYLNADNSVFDEEGYFATGDLVELYKENEEEYIKIIGRSKELINVGGE
CEEEEEECCCCCCEECCCCCCCCCCCCEECCHHHHHHHCCHHHHHHHHCCCHHHHHCCCC
KVVPQEVEGVILELDYIQDCLVYAKANAITGQSVCVKVILKANVEFTNLELKKQLRMYCK
CCCCHHHCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
DKLANYKIPTQVEIVEKLELSQRFKKVRK
HHHCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHNLNFSHPFLNKLIAYKDLNCLIYKDKTYSYLELLLEVKKYISQLNNLKGVIGILGDYN
CCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
LQSIALLLACIEKRLIVAPFLKLKNQDFKELEDKIYEGQIDYLYDEGKFIPYQSKQLKHN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LINTLQNHSSSGMILFSSGSTGKPKAMVHNLDVILNVYQDKKPKVMNIVLFLMFDHIGGL
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
NTLFNVLGMGACGIALEDRKNVEALAQNIQKYQVSLLPASPSLLNLILMSGVKEKYDLNS
HHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCC
LKLITYGTEKMSDSLLNRLKLEFPKVRFHQTFGTSEVGIAQTKTKDNMIKLEGMAYKIIN
EEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCCEEEECCEEEEEEC
NELYLKSKTQSLGYLNADNSVFDEEGYFATGDLVELYKENEEEYIKIIGRSKELINVGGE
CEEEEEECCCCCCEECCCCCCCCCCCCEECCHHHHHHHCCHHHHHHHHCCCHHHHHCCCC
KVVPQEVEGVILELDYIQDCLVYAKANAITGQSVCVKVILKANVEFTNLELKKQLRMYCK
CCCCHHHCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
DKLANYKIPTQVEIVEKLELSQRFKKVRK
HHHCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371453; 10952301 [H]