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The map label for this gene is hpaIM [H]

Identifier: 153951324

GI number: 153951324

Start: 846972

End: 847724

Strand: Direct

Name: hpaIM [H]

Synonym: JJD26997_0951

Alternate gene names: 153951324

Gene position: 846972-847724 (Clockwise)

Preceding gene: 153951746

Following gene: 153952357

Centisome position: 45.9

GC content: 33.6

Gene sequence:

>753_bases
ATGCAATGCGAAATTGTTAATTCGGACATAATTGAATATATCACAAAAATTGCAGATAACAGTATTGATCTGATAATTGC
TGATCCGCCTTATTTTCAAATCAAAGGCGAATTTGATTTTGGTGTGTTCAAAGACAAACATGAGTATCTTAGTTGGTGCA
AAAAATGGCTTATTGAATCAAAAAGAATTTTGAAAGATACAGGTTCAATGATTCTTTGGGGCAGTGTCGGTAATAGAGAA
ATCACTTTTGCAAGACTTGCGATAATGATTGAAGATGAAAATATTTTTTTGCGCAAAAATTGGATAACACAACGCAATAC
TCGTGGAATAGGCACAAAAACAAATTACATGTCGGTAAGGGAAGATTTTTTGTTTCTGACTAAAAGCAACAATTATACCT
TTAATATACCCTATACAAATGAAAAATCGACTAGAAAAGATTTTGGTGCAAATGGAAAACCTAGAAAAAATACTCACAAA
AGAGTTTCAAATGTTTGGGCTGACATTGCAGAAGCTAGTCAATCAAGCATAGAGCGTTGCAATCACCCAACCGTGAAAGC
TCAAAAACTTTGTGATAGAATAATTCAAACTCACTCAAATGAAGGCGACACTATTTTCGTTCCTTTTGTAGGAAGTGGGA
GCGAGATAATTTCAGCCATAAGAAACAATAGAAAAGCCTTTGGTTGCGAAATTAATAAAGAATATTGCAATTTGGCAAAA
GACAGAGTGAATATGCTGTTAAAAAGGGCATAA

Upstream 100 bases:

>100_bases
TTAGTGCATTATTATTTATAAGTATCATTGTGTTCTGTCATAACGACTTTTGCAATTCGCTAAATATGTTATAATTCATT
CAAAAAGATAAGGATTTGCT

Downstream 100 bases:

>100_bases
AAATGGAGTGGATAGATACTAGAAAAAAAAATCATCAATTTGTAAAATTCATAGTTAATAACCATAAAAAAGAATTAGAG
TGGGCTGTAAAAACAGCTTC

Product: DNA methylase

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MQCEIVNSDIIEYITKIADNSIDLIIADPPYFQIKGEFDFGVFKDKHEYLSWCKKWLIESKRILKDTGSMILWGSVGNRE
ITFARLAIMIEDENIFLRKNWITQRNTRGIGTKTNYMSVREDFLFLTKSNNYTFNIPYTNEKSTRKDFGANGKPRKNTHK
RVSNVWADIAEASQSSIERCNHPTVKAQKLCDRIIQTHSNEGDTIFVPFVGSGSEIISAIRNNRKAFGCEINKEYCNLAK
DRVNMLLKRA

Sequences:

>Translated_250_residues
MQCEIVNSDIIEYITKIADNSIDLIIADPPYFQIKGEFDFGVFKDKHEYLSWCKKWLIESKRILKDTGSMILWGSVGNRE
ITFARLAIMIEDENIFLRKNWITQRNTRGIGTKTNYMSVREDFLFLTKSNNYTFNIPYTNEKSTRKDFGANGKPRKNTHK
RVSNVWADIAEASQSSIERCNHPTVKAQKLCDRIIQTHSNEGDTIFVPFVGSGSEIISAIRNNRKAFGCEINKEYCNLAK
DRVNMLLKRA
>Mature_250_residues
MQCEIVNSDIIEYITKIADNSIDLIIADPPYFQIKGEFDFGVFKDKHEYLSWCKKWLIESKRILKDTGSMILWGSVGNRE
ITFARLAIMIEDENIFLRKNWITQRNTRGIGTKTNYMSVREDFLFLTKSNNYTFNIPYTNEKSTRKDFGANGKPRKNTHK
RVSNVWADIAEASQSSIERCNHPTVKAQKLCDRIIQTHSNEGDTIFVPFVGSGSEIISAIRNNRKAFGCEINKEYCNLAK
DRVNMLLKRA

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=251, Percent_Identity=32.6693227091633, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 28865; Mature: 28865

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCEIVNSDIIEYITKIADNSIDLIIADPPYFQIKGEFDFGVFKDKHEYLSWCKKWLIES
CCEEEECHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KRILKDTGSMILWGSVGNREITFARLAIMIEDENIFLRKNWITQRNTRGIGTKTNYMSVR
HHHHHCCCCEEEEECCCCCEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHEEC
EDFLFLTKSNNYTFNIPYTNEKSTRKDFGANGKPRKNTHKRVSNVWADIAEASQSSIERC
CCEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NHPTVKAQKLCDRIIQTHSNEGDTIFVPFVGSGSEIISAIRNNRKAFGCEINKEYCNLAK
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCEECEEECHHHHHHHH
DRVNMLLKRA
HHHHHHHHCC
>Mature Secondary Structure
MQCEIVNSDIIEYITKIADNSIDLIIADPPYFQIKGEFDFGVFKDKHEYLSWCKKWLIES
CCEEEECHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KRILKDTGSMILWGSVGNREITFARLAIMIEDENIFLRKNWITQRNTRGIGTKTNYMSVR
HHHHHCCCCEEEEECCCCCEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHEEC
EDFLFLTKSNNYTFNIPYTNEKSTRKDFGANGKPRKNTHKRVSNVWADIAEASQSSIERC
CCEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NHPTVKAQKLCDRIIQTHSNEGDTIFVPFVGSGSEIISAIRNNRKAFGCEINKEYCNLAK
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCCEECEEECHHHHHHHH
DRVNMLLKRA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]