Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 153951319

GI number: 153951319

Start: 1485485

End: 1485970

Strand: Direct

Name: greA [H]

Synonym: JJD26997_1684

Alternate gene names: 153951319

Gene position: 1485485-1485970 (Clockwise)

Preceding gene: 153952025

Following gene: 153951836

Centisome position: 80.51

GC content: 35.8

Gene sequence:

>486_bases
ATGCAAAAAGAACCAATGAGTCAATTTGGCTATGATAAATTAGCCGCAGAATTAAAAGATTTAAAAGACAATCAACGCCC
TGCTGTTGTAATAGAAATCGATACAGCAAGGAGTCATGGGGATTTGAAAGAAAATGCTGAATACCATGCTGCAAGAGAAA
AGCAAGCTTTGATAGAAAGTCGTATTGTAGAGCTTAGTGACTTGCTAGCAAGAGCTCAAGTGATCGATCCTTCAAGCTAT
GAGCACGATAGTGTGAAATTTGGTTCAAGTGTTGTGATCATGGATTTGGATACAGAAAAAGAAAGCAAATATACTCTAGT
AGGAATTTGCGAAGGAGATTTAGATAAAGGTTATATTTCCATCGCATCTCCAATAGCAAGAGCTATGCTTGGAAAAAAAG
AAGGAGATGATTTTAAAGTGCGTTTGCCAAAAGGTGAGAGTGAATTTGAAATCCTTTCTATAAATTATGAGCCTTTGAAA
TTTTAA

Upstream 100 bases:

>100_bases
AGGCATTTTTTGCTAAAGTTGATTTTTTAAAAGAATATTTACAATTTGGAAGTGCTAAAAATTTAGCAAAAATATTGAGT
GAAATTTAAAAGGATAAATC

Downstream 100 bases:

>100_bases
GGATAAAAAAATTTTACTACAAAATGGAGCAATTTTAATCGCAGATGCTCATGAAAATGAACAAAGAAAGGGATTTTGGG
AATTTTTACAAGCCTTAAAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIESRIVELSDLLARAQVIDPSSY
EHDSVKFGSSVVIMDLDTEKESKYTLVGICEGDLDKGYISIASPIARAMLGKKEGDDFKVRLPKGESEFEILSINYEPLK
F

Sequences:

>Translated_161_residues
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIESRIVELSDLLARAQVIDPSSY
EHDSVKFGSSVVIMDLDTEKESKYTLVGICEGDLDKGYISIASPIARAMLGKKEGDDFKVRLPKGESEFEILSINYEPLK
F
>Mature_161_residues
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIESRIVELSDLLARAQVIDPSSY
EHDSVKFGSSVVIMDLDTEKESKYTLVGICEGDLDKGYISIASPIARAMLGKKEGDDFKVRLPKGESEFEILSINYEPLK
F

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=45.2229299363057, Blast_Score=139, Evalue=7e-35,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=34.6153846153846, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18068; Mature: 18068

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIES
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
RIVELSDLLARAQVIDPSSYEHDSVKFGSSVVIMDLDTEKESKYTLVGICEGDLDKGYIS
HHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEEEECCCCCCCEEEEEEECCCCCCCEEH
IASPIARAMLGKKEGDDFKVRLPKGESEFEILSINYEPLKF
HHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MQKEPMSQFGYDKLAAELKDLKDNQRPAVVIEIDTARSHGDLKENAEYHAAREKQALIES
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
RIVELSDLLARAQVIDPSSYEHDSVKFGSSVVIMDLDTEKESKYTLVGICEGDLDKGYIS
HHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEEEECCCCCCCEEEEEEECCCCCCCEEH
IASPIARAMLGKKEGDDFKVRLPKGESEFEILSINYEPLKF
HHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA