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The map label for this gene is gatB [H]

Identifier: 153951134

GI number: 153951134

Start: 478892

End: 480310

Strand: Direct

Name: gatB [H]

Synonym: JJD26997_0532

Alternate gene names: 153951134

Gene position: 478892-480310 (Clockwise)

Preceding gene: 153952326

Following gene: 153952074

Centisome position: 25.95

GC content: 34.74

Gene sequence:

>1419_bases
ATGTTCGAAGTAGTTATAGGTCTTGAAGTTCATACTCAGTTAAATACAAAAACAAAAATCTTTTGCTCATGCGCAACTTC
TTTTGGAGAAGCTCCAAATACTAATGTTTGTCCCACTTGTTTGGCCTTACCAGGAGCTTTGCCTGTTTTAAATGAAGAGG
CCGTAAAAAAAGCTATAGCTTTTGGAAAAGCTGTAAATGCAGCAATTAATAAAAAAAGTGTTTTTAACCGTAAGAATTAT
TTTTATCCAGATTTACCTAAAGCCTATCAAATTTCACAATTTGATATCCCTATAGTAGAAAAGGGTGAGCTTTTTATCAA
TGTGAATGGCGAAAATAAACGCATAGGCATTACAAGGGCGCATTTAGAAGAGGATGCGGGTAAAAATATCCACGAAAATA
ATTTTTCTAAAGTGGACTTAAATCGTGCAGGAACTCCTTTGCTTGAGATTGTCAGCGAACCTGAGCTTAGAAGTAGCGAT
GAGGCAGTAGCTTATCTTAAGAAACTTCATTCTATTATACGCTTTTTAGACATTTCAGATGCAAATATGCAAGAAGGAAG
TTTTAGATGCGATGCAAATGTTAGTATAAGACCTAAGGGTGATACAAAACTTTATACTAGGGTAGAGATTAAAAATCTTA
ATTCTTTTCGTTTTATCCAAAAAGCCATAGATTTTGAAGTCAAAAGACAAAGTGAAGCTTGGGAAGATGGCACTTATGAG
CAAGAAGTGGTGCAAGAAACAAGACTTTTTGACACTACAAATTTAGTTACAAGAAGTATGCGTGGTAAAGAAGAAGCGGC
TGAATATCGTTATTTTCCAGACCCTGACTTGCTTCCTGTTTTGTTAAAAGATGAGTTTTTGCAAATCAAAATCCCTGAAC
TTCCTGATGAGAAAAAAATGCGTTTTGTCAGTGAGTTGGGTATAAAAGAAAGTGATGCTGAAGTGATAATTAGCTCACTA
GAAATGAGTCGTTTTTTTGAAAGTCTTATTTCTCAAAATTTAAGCCCTAAGCTTTGTGTAAATTGGTTAAATACTGAACT
TATGGGATTTTTAAAAGGAGAGTTGACTATAGAAAATTCTCCCGTAGATGCTCAAAAACTTGGAGATTTGATTAAACGCA
TAGAAGATGGCACCATCAGTGCAAAAGCGGCTAAAGATGTTTTGGCTTTTGTGTTTGAAAATACTAACGTGGAAATTGAT
GAAGCTATAGAAAAGCTAGGACTTAAACAAGTAAGTGATGACTCAGCTATAGAAGCGGTGATTGAACAAATCTTAAATGC
AAATGCCGATAAGGTTGCAGAGTATAAAAGTGGTAAAGACAAGCTTTTTGGATTCTTTGTGGGTCAAACAATGAAAGAAG
GCAAAAGAGCTTTTAATCCTGCAAAAGTGAATGAAATTTTAAAAGCAAAGCTTGGTTGA

Upstream 100 bases:

>100_bases
TATAAATTGTCTAAGGATTATAGCATTTAAAGTTAATATTTAAAGCCTTTCTAAAAGAAAAAAAGATAAAATAAAACATT
TAAAAATTCAAGGAAATTTT

Downstream 100 bases:

>100_bases
TGCGTATAGCGGTTATTGGTGCGGGAAAATGGGGCAGTGCTTTACATCTAGCTTTAAAAGAAAATCACACGTGTTTTATC
AGTTCTTTGCATCAGCGTGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIAFGKAVNAAINKKSVFNRKNY
FYPDLPKAYQISQFDIPIVEKGELFINVNGENKRIGITRAHLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSD
EAVAYLKKLHSIIRFLDISDANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIDFEVKRQSEAWEDGTYE
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLQIKIPELPDEKKMRFVSELGIKESDAEVIISSL
EMSRFFESLISQNLSPKLCVNWLNTELMGFLKGELTIENSPVDAQKLGDLIKRIEDGTISAKAAKDVLAFVFENTNVEID
EAIEKLGLKQVSDDSAIEAVIEQILNANADKVAEYKSGKDKLFGFFVGQTMKEGKRAFNPAKVNEILKAKLG

Sequences:

>Translated_472_residues
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIAFGKAVNAAINKKSVFNRKNY
FYPDLPKAYQISQFDIPIVEKGELFINVNGENKRIGITRAHLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSD
EAVAYLKKLHSIIRFLDISDANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIDFEVKRQSEAWEDGTYE
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLQIKIPELPDEKKMRFVSELGIKESDAEVIISSL
EMSRFFESLISQNLSPKLCVNWLNTELMGFLKGELTIENSPVDAQKLGDLIKRIEDGTISAKAAKDVLAFVFENTNVEID
EAIEKLGLKQVSDDSAIEAVIEQILNANADKVAEYKSGKDKLFGFFVGQTMKEGKRAFNPAKVNEILKAKLG
>Mature_472_residues
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIAFGKAVNAAINKKSVFNRKNY
FYPDLPKAYQISQFDIPIVEKGELFINVNGENKRIGITRAHLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSD
EAVAYLKKLHSIIRFLDISDANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIDFEVKRQSEAWEDGTYE
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLQIKIPELPDEKKMRFVSELGIKESDAEVIISSL
EMSRFFESLISQNLSPKLCVNWLNTELMGFLKGELTIENSPVDAQKLGDLIKRIEDGTISAKAAKDVLAFVFENTNVEID
EAIEKLGLKQVSDDSAIEAVIEQILNANADKVAEYKSGKDKLFGFFVGQTMKEGKRAFNPAKVNEILKAKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=34.20523138833, Blast_Score=283, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI115534094, Length=313, Percent_Identity=38.3386581469649, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319391, Length=509, Percent_Identity=30.2554027504912, Blast_Score=199, Evalue=9e-52,
Organism=Drosophila melanogaster, GI45550808, Length=481, Percent_Identity=32.2245322245322, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52995; Mature: 52995

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIA
CEEEEEEEEEEEECCCCEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
FGKAVNAAINKKSVFNRKNYFYPDLPKAYQISQFDIPIVEKGELFINVNGENKRIGITRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCEEECCEEEEEECCCCCEEEEEHH
HLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSDEAVAYLKKLHSIIRFLDISD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIDFEVKRQSEAWEDGTYE
CCCCCCCEEECCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLQIKIPELPDEKKM
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHEECCCEEEEECCCCCCHHHH
RFVSELGIKESDAEVIISSLEMSRFFESLISQNLSPKLCVNWLNTELMGFLKGELTIENS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECC
PVDAQKLGDLIKRIEDGTISAKAAKDVLAFVFENTNVEIDEAIEKLGLKQVSDDSAIEAV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHH
IEQILNANADKVAEYKSGKDKLFGFFVGQTMKEGKRAFNPAKVNEILKAKLG
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MFEVVIGLEVHTQLNTKTKIFCSCATSFGEAPNTNVCPTCLALPGALPVLNEEAVKKAIA
CEEEEEEEEEEEECCCCEEEEEEEHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
FGKAVNAAINKKSVFNRKNYFYPDLPKAYQISQFDIPIVEKGELFINVNGENKRIGITRA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCEEECCEEEEEECCCCCEEEEEHH
HLEEDAGKNIHENNFSKVDLNRAGTPLLEIVSEPELRSSDEAVAYLKKLHSIIRFLDISD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ANMQEGSFRCDANVSIRPKGDTKLYTRVEIKNLNSFRFIQKAIDFEVKRQSEAWEDGTYE
CCCCCCCEEECCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
QEVVQETRLFDTTNLVTRSMRGKEEAAEYRYFPDPDLLPVLLKDEFLQIKIPELPDEKKM
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHEECCCEEEEECCCCCCHHHH
RFVSELGIKESDAEVIISSLEMSRFFESLISQNLSPKLCVNWLNTELMGFLKGELTIENS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECC
PVDAQKLGDLIKRIEDGTISAKAAKDVLAFVFENTNVEIDEAIEKLGLKQVSDDSAIEAV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHH
IEQILNANADKVAEYKSGKDKLFGFFVGQTMKEGKRAFNPAKVNEILKAKLG
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA