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The map label for this gene is gltJ [H]

Identifier: 153949784

GI number: 153949784

Start: 4700282

End: 4701019

Strand: Reverse

Name: gltJ [H]

Synonym: YpsIP31758_4164

Alternate gene names: 153949784

Gene position: 4701019-4700282 (Counterclockwise)

Preceding gene: 153946989

Following gene: 153948328

Centisome position: 99.53

GC content: 49.32

Gene sequence:

>738_bases
ATGAATCTACATCATCTGACTGATTGGCTATTAGCCCCGCAATATCTTGGTTGGTTGTGGGATGGTTTCCTACTGACGTT
GTGGATTTCCGCCTGTACGGTGGTGGCTGCCACGTTATTGGGATTTTTGCTGGCCGCGGCCAGAGATAGCGAGTTAAAAG
CCTTGAAAGGCTTTGCTATTGGCTACAGTGCCTTATTCCGAAATACTCCTCTGCTGATCCAATTATTCTTTTGGTACTTT
GCTGCCAGCCAATTCCTGCCAGCTTCTTGGATCCCTTGGCTCAATACTCCCCATGAAATTACGGTTGCCGAATTAACTCT
GGCCTGGCCCTCTTTTGAGTTTTTAGCCGGTTTTGTCGGGTTGACACTCTATTCCACCGCATTTATTGCTGAAGAGCTGC
GTGCGGGCATCAGCGGCGTAGCCAAGGGGCAGAAATATGCTGCACATGCGCTGGGGCTGACGGGGTGGCAATCGATGCGG
TATGTGGTTTTGCCGCAGGCTTTGAAAATAGCCATGCCTCCGTTGCTGGGCCAATATATGAATATTATTAAAAACTCTTC
ATTGGCAATGGCGATTGGTGTAGCTGAACTCTCCTATGCTTCCCGGCAGGTTGAAACTGAAACATTACGGACGTTTCAGG
CATTCGGGGTTGCAACCCTATTGTATATTTTCATCATCGCGCTAATTGAGGCTTGGGGTATGTGGCGTCAACAGCGAACT
GTCGTCCGAGGGCACTGA

Upstream 100 bases:

>100_bases
GTCGGTATATTAAGTTATGAAGTAAAAATAAGATGGATTTTATTGTGTACTTAAAAACTCATGCGATTAGAGCATCAGAT
GGATATAGATATAAAAGCCT

Downstream 100 bases:

>100_bases
GATGGATTTTACCATTATCGTTGATAACTGGCGTTATCTGCTGTGGGGGACTTTCCCTGATGGCCCTCTGGGAGGGGCGG
CATTGACCGTGGTGATCAGT

Product: polar amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNLHHLTDWLLAPQYLGWLWDGFLLTLWISACTVVAATLLGFLLAAARDSELKALKGFAIGYSALFRNTPLLIQLFFWYF
AASQFLPASWIPWLNTPHEITVAELTLAWPSFEFLAGFVGLTLYSTAFIAEELRAGISGVAKGQKYAAHALGLTGWQSMR
YVVLPQALKIAMPPLLGQYMNIIKNSSLAMAIGVAELSYASRQVETETLRTFQAFGVATLLYIFIIALIEAWGMWRQQRT
VVRGH

Sequences:

>Translated_245_residues
MNLHHLTDWLLAPQYLGWLWDGFLLTLWISACTVVAATLLGFLLAAARDSELKALKGFAIGYSALFRNTPLLIQLFFWYF
AASQFLPASWIPWLNTPHEITVAELTLAWPSFEFLAGFVGLTLYSTAFIAEELRAGISGVAKGQKYAAHALGLTGWQSMR
YVVLPQALKIAMPPLLGQYMNIIKNSSLAMAIGVAELSYASRQVETETLRTFQAFGVATLLYIFIIALIEAWGMWRQQRT
VVRGH
>Mature_245_residues
MNLHHLTDWLLAPQYLGWLWDGFLLTLWISACTVVAATLLGFLLAAARDSELKALKGFAIGYSALFRNTPLLIQLFFWYF
AASQFLPASWIPWLNTPHEITVAELTLAWPSFEFLAGFVGLTLYSTAFIAEELRAGISGVAKGQKYAAHALGLTGWQSMR
YVVLPQALKIAMPPLLGQYMNIIKNSSLAMAIGVAELSYASRQVETETLRTFQAFGVATLLYIFIIALIEAWGMWRQQRT
VVRGH

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=216, Percent_Identity=35.1851851851852, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI48994930, Length=306, Percent_Identity=29.7385620915033, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1788226, Length=208, Percent_Identity=35.0961538461538, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786873, Length=189, Percent_Identity=31.7460317460317, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787030, Length=221, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082239, Length=205, Percent_Identity=29.2682926829268, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788645, Length=217, Percent_Identity=27.6497695852535, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787087, Length=210, Percent_Identity=22.8571428571429, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27349; Mature: 27349

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHHLTDWLLAPQYLGWLWDGFLLTLWISACTVVAATLLGFLLAAARDSELKALKGFAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GYSALFRNTPLLIQLFFWYFAASQFLPASWIPWLNTPHEITVAELTLAWPSFEFLAGFVG
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHH
LTLYSTAFIAEELRAGISGVAKGQKYAAHALGLTGWQSMRYVVLPQALKIAMPPLLGQYM
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
NIIKNSSLAMAIGVAELSYASRQVETETLRTFQAFGVATLLYIFIIALIEAWGMWRQQRT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVRGH
HHCCC
>Mature Secondary Structure
MNLHHLTDWLLAPQYLGWLWDGFLLTLWISACTVVAATLLGFLLAAARDSELKALKGFAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GYSALFRNTPLLIQLFFWYFAASQFLPASWIPWLNTPHEITVAELTLAWPSFEFLAGFVG
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHH
LTLYSTAFIAEELRAGISGVAKGQKYAAHALGLTGWQSMRYVVLPQALKIAMPPLLGQYM
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
NIIKNSSLAMAIGVAELSYASRQVETETLRTFQAFGVATLLYIFIIALIEAWGMWRQQRT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVRGH
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]