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The map label for this gene is hemL

Identifier: 153949232

GI number: 153949232

Start: 3742867

End: 3744147

Strand: Direct

Name: hemL

Synonym: YpsIP31758_3330

Alternate gene names: 153949232

Gene position: 3742867-3744147 (Clockwise)

Preceding gene: 153947152

Following gene: 153949525

Centisome position: 79.24

GC content: 50.98

Gene sequence:

>1281_bases
ATGAGTAAATCCGAAAATCTGTATGCCCAAGCACAACAGTTGATCCCGGGCGGGGTTAACTCTCCGGTGCGTGCATTCAC
AGGTGTTGGGGGCATCCCACTCTTTATTGAACGTGCTGATGGTGCTTACCTCTTTGACGTAGATGGCAAAGCCTATATTG
ACTATGTCGGCTCTTGGGGGCCAATGATCTTGGGCCATAATCACCCTGCTATTCGTCAGGCGGTCATTGAGGCCGTTGAA
CGTGGCCTAAGTTTTGGTGCACCGACAGAGATGGAAGTGAAAATGGCTCAGTTGGTTACAGATTTGGTGCCAACCATGGA
TATGGTACGGATGGTCAATTCCGGCACCGAAGCCACCATGAGTGCCATCCGTCTGGCCCGAGGCTATACCGGTCGCGATA
AGATAATTAAATTCGAAGGCTGCTATCACGGCCATGCCGATTGTTTGTTGGTAAAAGCAGGCTCAGGCGCACTGACACTG
GGTCAGCCCAATTCACCTGGTGTTCCTGCGGATTTTGCCAAACACACCCTGACCTGTACCTATAACGATCTGGCTTCTGT
ACGGCAGGCCTTTGAGCAATATCCGCAAGAAGTGGCGTGTATTATCGTTGAGCCAGTAGCCGGTAATATGAATTGCATTC
CCCCGTTGCCAGAGTTCCTGCCTGGTCTGCGCGCTTTGTGTGATGAGTTTGGTGCTCTGCTTATCATCGATGAAGTCATG
ACCGGTTTTCGCGTAGCCTTAGCTGGCGCGCAGGATTATTACCATGTCATCCCTGATTTAACCTGTCTGGGTAAAATCAT
TGGTGGCGGTATGCCAGTAGGCGCTTTTGGTGGCCGCCGTGAGGTAATGAATGCGCTGGCACCGACTGGCCCGGTTTACC
AAGCCGGTACGCTATCCGGTAACCCTATCGCTATGGCTGCCGGTTTTGCCTGCCTGACAGAAATCTCTCAGGTCGGTGTT
TATGAAACACTGACCGAATTAACCGACTCACTGGCTACAGGCCTGCGACATGCCGCTAAAGAGGAGAACATCCCACTGGT
GGTTAACCACGTCGGTGGGATGTTTGGCCTATTCTTTACCAATGCTGATACCGTCACCTGCTATCAGGATGTGATGAATT
GTGATGTTGAGCGCTTTAAACGTTTCTTCCATTTGATGCTGGAAGAAGGGGTCTATCTGGCACCGTCAGCCTTTGAAGCG
GGCTTTATGTCTCTGGCTCACAGCAACGAAGATATTCAGAAAACCGTCAATGCCGCTCGTCGATGCTTTGCTAAACTTTA
A

Upstream 100 bases:

>100_bases
CATATTGTCACAAGAACGACGTTACCAAGGCTAAGGCTGCCTAGATGAATAATGTTGCCAAAGTGAATAACGTTGCCAAG
CCCAATTCAGGAGCCAATTT

Downstream 100 bases:

>100_bases
TTCAATATTTGTTTGAATTTTAGTCAAAACAATATCTATTTGAGTTTTAGCCCAAAATAGAAGGTAATCAGACTGCTGAC
AAACCCGAACACTCGATCTT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSKSENLYAQAQQLIPGGVNSPVRAFTGVGGIPLFIERADGAYLFDVDGKAYIDYVGSWGPMILGHNHPAIRQAVIEAVE
RGLSFGAPTEMEVKMAQLVTDLVPTMDMVRMVNSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRQAFEQYPQEVACIIVEPVAGNMNCIPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYHVIPDLTCLGKIIGGGMPVGAFGGRREVMNALAPTGPVYQAGTLSGNPIAMAAGFACLTEISQVGV
YETLTELTDSLATGLRHAAKEENIPLVVNHVGGMFGLFFTNADTVTCYQDVMNCDVERFKRFFHLMLEEGVYLAPSAFEA
GFMSLAHSNEDIQKTVNAARRCFAKL

Sequences:

>Translated_426_residues
MSKSENLYAQAQQLIPGGVNSPVRAFTGVGGIPLFIERADGAYLFDVDGKAYIDYVGSWGPMILGHNHPAIRQAVIEAVE
RGLSFGAPTEMEVKMAQLVTDLVPTMDMVRMVNSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCTYNDLASVRQAFEQYPQEVACIIVEPVAGNMNCIPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYHVIPDLTCLGKIIGGGMPVGAFGGRREVMNALAPTGPVYQAGTLSGNPIAMAAGFACLTEISQVGV
YETLTELTDSLATGLRHAAKEENIPLVVNHVGGMFGLFFTNADTVTCYQDVMNCDVERFKRFFHLMLEEGVYLAPSAFEA
GFMSLAHSNEDIQKTVNAARRCFAKL
>Mature_425_residues
SKSENLYAQAQQLIPGGVNSPVRAFTGVGGIPLFIERADGAYLFDVDGKAYIDYVGSWGPMILGHNHPAIRQAVIEAVER
GLSFGAPTEMEVKMAQLVTDLVPTMDMVRMVNSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHADCLLVKAGSGALTLG
QPNSPGVPADFAKHTLTCTYNDLASVRQAFEQYPQEVACIIVEPVAGNMNCIPPLPEFLPGLRALCDEFGALLIIDEVMT
GFRVALAGAQDYYHVIPDLTCLGKIIGGGMPVGAFGGRREVMNALAPTGPVYQAGTLSGNPIAMAAGFACLTEISQVGVY
ETLTELTDSLATGLRHAAKEENIPLVVNHVGGMFGLFFTNADTVTCYQDVMNCDVERFKRFFHLMLEEGVYLAPSAFEAG
FMSLAHSNEDIQKTVNAARRCFAKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=312, Percent_Identity=30.4487179487179, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI13994255, Length=373, Percent_Identity=26.0053619302949, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI284507298, Length=230, Percent_Identity=31.304347826087, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=88.7323943661972, Blast_Score=792, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=384, Percent_Identity=31.7708333333333, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787560, Length=348, Percent_Identity=31.8965517241379, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI145693181, Length=313, Percent_Identity=31.3099041533546, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788044, Length=332, Percent_Identity=30.7228915662651, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789759, Length=332, Percent_Identity=29.8192771084337, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786991, Length=298, Percent_Identity=27.1812080536913, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=341, Percent_Identity=27.5659824046921, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71992977, Length=363, Percent_Identity=28.099173553719, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=329, Percent_Identity=25.8358662613982, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144274, Length=183, Percent_Identity=31.6939890710383, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=341, Percent_Identity=28.7390029325513, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324432, Length=351, Percent_Identity=26.7806267806268, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357415, Length=320, Percent_Identity=26.5625, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356575, Length=329, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_YERP3 (A7FM09)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402287.1
- ProteinModelPortal:   A7FM09
- SMR:   A7FM09
- STRING:   A7FM09
- GeneID:   5384523
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3330
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   YPSE349747:YPSIP31758_3330-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45764; Mature: 45633

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSENLYAQAQQLIPGGVNSPVRAFTGVGGIPLFIERADGAYLFDVDGKAYIDYVGSWG
CCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMILGHNHPAIRQAVIEAVERGLSFGAPTEMEVKMAQLVTDLVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRQAFEQYPQEVACIIVEPVAGNMNCIPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYHVIPDLTCLGKIIGGGMPVGAFGGRREVMNALAPTGPVYQAGTLSG
HHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLTEISQVGVYETLTELTDSLATGLRHAAKEENIPLVVNHVGGMFGLFFT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCC
NADTVTCYQDVMNCDVERFKRFFHLMLEEGVYLAPSAFEAGFMSLAHSNEDIQKTVNAAR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHH
RCFAKL
HHHHCC
>Mature Secondary Structure 
SKSENLYAQAQQLIPGGVNSPVRAFTGVGGIPLFIERADGAYLFDVDGKAYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEECCCCC
PMILGHNHPAIRQAVIEAVERGLSFGAPTEMEVKMAQLVTDLVPTMDMVRMVNSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKHTLTCT
HHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEECCCCCCCCCCHHHHHCEEEEE
YNDLASVRQAFEQYPQEVACIIVEPVAGNMNCIPPLPEFLPGLRALCDEFGALLIIDEVM
HHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEHHHHH
TGFRVALAGAQDYYHVIPDLTCLGKIIGGGMPVGAFGGRREVMNALAPTGPVYQAGTLSG
HHHHHHHCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGFACLTEISQVGVYETLTELTDSLATGLRHAAKEENIPLVVNHVGGMFGLFFT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHCC
NADTVTCYQDVMNCDVERFKRFFHLMLEEGVYLAPSAFEAGFMSLAHSNEDIQKTVNAAR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHH
RCFAKL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA