Click here to switch to the map view.

The map label for this gene is ptsA [H]

Identifier: 153949115

GI number: 153949115

Start: 3981682

End: 3984258

Strand: Reverse

Name: ptsA [H]

Synonym: YpsIP31758_3528

Alternate gene names: 153949115

Gene position: 3984258-3981682 (Counterclockwise)

Preceding gene: 153948707

Following gene: 153949683

Centisome position: 84.35

GC content: 52.58

Gene sequence:

>2577_bases
ATGACCAAGCAAGTGACATTTATCTGCGGGCTTCCTAATGGCGTTCATGCCCGACCCGCGAGCCATATAGAAAGGGTGTG
CAACCGCTTCCAGTCGCGTTTCCACTGGTATAATCCGCGTTCTGGCATCGTGGGGGATGGCAAGAGCGTACTTTCGCTGA
TCGCGACCAATATCCTGTTAGGGGATGAATGTCAGGTCACCATTGAAGGCGAAGATGAGCAGGCTGCTTTTGAACGCCTG
AGTATTTTTATTGAGCACGAGCTTCCACACGCTGACCCCATCTTGCCCAAGAGGGAGGAGAGTGCAGAATGGGAGCCGCT
CCCCGCCTCGTTAGCCCACTTGCACCCCACCCTGTTACGCGCCCGTTCAGTCAGTCCAGGTACCGCCTGCGGGAAGTTGC
TGTCTCTGATAAGAGCCGATCTCAATGCATTGGGTGATTTACCCGTGGCGCAAGGTATCGAGAGGGAGCAGCAGATGCTT
GCCGATGGGGTGGCGCAGCTAGGTAAAGCGTGGGAGAGCTTATTAGTCGCCAATAGCAGTACGGCAGCCAATAGCAGTAC
GACAGAAAATAGCAGTACGACAGAAAATAACAGTACGACAGAAAATAACAGCACGACGCGAGCGATACGGGAAGTACACC
GCTCGCTGTTGCGTGATGGCACATTTCGTCAACGGCTCTTGAGTCACATAATAGCAGGTGAAAGCTGTGCGACAGCCATC
GTCGCTACTGCGGCCTATTTCAGCCAGCAGTTGGCGCTTGCTGCTAACACTTATCTACGTGAACGTGAGTTGGATATTCG
AGACGTCAGTTTTCAATTGTTACAGCAGATTTATGGTGAGCAACGTTTTCCCTCCCAGCAAGCATTGAGCGAAGACAGTC
TCTGCATTGCCGATGAGTTAACCCCGAGCCAATTCCTGGCGCTGGATAAACGTTATCTAAAAGGGTTGCTATTGGGCCGT
GGTGGCAGTACTTCACATACGGTGATTCTGGCGCGTTCATTCAATATTCCGACGCTCGTGGGTGTCGATGCTACGGCACT
CCAGCCGTATCTCAATCAATCGCTGCAGATTGATGGTGAGCTTGGGCTGGTGGTCTGTCTTTTGGATGAGCCGGTGCGCC
GTTACTATCGGCAGGAGCAGTGGCTACACGACCAACTGCGTGAGCAGCAGAGCCGATACCAGAATATGCCTGGGCGTACT
CTTGATGGTGTCAGAATGGTGGTGGCGGCTAACATCACCCATGCGGTAGAGGTGGAGGGTGCTTTCAATCAAGGTGCCGA
ATCCATCGGCTTGTTTCGTACTGAAATGTTGTATATGGATCGCGCAGCCGCGCCTTCAGAAGAGGAGCTTTATACCCTTT
ACGCTCAGGCCCTTGGCGCTGCCAAGGGCAAACCGATGATTATTCGCACCATCGATATTGGCGGTGATAAACCGGTGAGT
TATCTGAATATTCCTGCGGAGAGCAACCCGTTTCTGGGCTACCGCGCGGTACGTATTTATCACGAGTTTCTGTCGCTATT
TCATACTCAATTGCGGGCGATCCTGCGGGCGTCAATGCATGGCCCGCTCAAAATCATGATCCCGATGATCTCCTCAATGG
AGGAGATCCTGTGGGTGAAAGATCAACTGGCAGAGGTGAAACAATCACTGCGTATCAATCATCTTCAGTTCGACGAAACT
GTCCCCTTGGGTATGATGCTGGAAGTGCCTTCAGTGATGTTTATCATCGATCAATGCTGTGAGGAAATGGATTTTTTGAG
TATTGGCAGTAACGACCTAACACAGTACCTGCTGGCTGTTGATCGCGATAATGCCAAGGTGAGTGAGCACTATCACTGCT
TGTCCCCGGCACTCCTACGCGCTCTCGATTACGCAGTGTGCGAAGTACATCGCCACGGTAAATGGATTGGCTTATGTGGT
GAACTGGCGGCAAAAGACTCGGTATTACCTCTGTTGGTAGCGATGGGGCTAGATGAGATCAGCATGAGTGCCTCTTTTAT
TGGCGCGACTAAAGCTCGCCTTGCCAAACTTGATCGCGGCGAGTGCCGCCTGCTGCTCAACCGGGCGATGGCTTGCCGCA
CATCACGGGAAGTAGAACATCTGCTGGTGCAGTACGACGGGGAACAAGGTGATGAACCACTGATTATTCCTGAATGTATT
ACTCTCGGTGTGGATTGGCGTAGCAAAGAAGAGGTGATCAAAGGGATGGTGGATAACCTGCTGCTGGCTGGACGTTGCCG
GTATCCGCGCAAACTGGCTGCGGATGTGTGGGCGCGAGAAGCCCTTTTTTCCACCGGATTGGGCTTTGGGTTTGCCATTC
CGCATGCTCAATCTGAGCATATTGAGCAGTCGACGATCAGCGTGGCCAAGTTGGCACAACCGGTGAATTGGGGAGAGGAA
GACGCACTGTTTGTGATGATGTTAACCCTGAAAAAACAGGCTTCGGGTGACCAGCATATGCGTATTTTTTCCAAACTGGC
GCGTCGGATCATGCACGAGGGTTTCCGCGAGGCGCTGGTTAGCGCTGGGACGGCCCAGCAAATAGAAACGCTGCTGAAAC
ACGAGCTCGAACTGTGA

Upstream 100 bases:

>100_bases
TGTGATTACATTTGTCCAGTATTTGACATTTTTATCTCATAGCCGCGAGGGGATAGCCGCTTTTATGCTGGTTACCAGCC
AGCGAGGGGAGGAAAGCCCA

Downstream 100 bases:

>100_bases
TTCTTTCATTTCAACATGACGGGTGCCGCGTCTGAGGTGGCACCCAGTGACCCTTATCGGATTACTTCAATAAGAATGAC
TTTCCTTGCTTAAGCACTAA

Product: HPr family phosphocarrier protein

Products: NA

Alternate protein names: MTP 2; Phosphoenolpyruvate-protein phosphotransferase; Enzyme I-Ani; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-like phosphotransferase enzyme IIA component; PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 858; Mature: 857

Protein sequence:

>858_residues
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTAANSSTTENSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGESCATAI
VATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGR
GGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRT
LDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVS
YLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINHLQFDET
VPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCG
ELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECI
TLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQPVNWGEE
DALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL

Sequences:

>Translated_858_residues
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTAANSSTTENSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGESCATAI
VATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGR
GGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRT
LDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVS
YLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINHLQFDET
VPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCG
ELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECI
TLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQPVNWGEE
DALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL
>Mature_857_residues
TKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERLS
IFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLA
DGVAQLGKAWESLLVANSSTAANSSTTENSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGESCATAIV
ATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRG
GSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTL
DGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVSY
LNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINHLQFDETV
PLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGE
LAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECIT
LGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQPVNWGEED
ALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=854, Percent_Identity=62.5292740046838, Blast_Score=1067, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=858, Percent_Identity=41.6083916083916, Blast_Score=626, Evalue=1e-180,
Organism=Escherichia coli, GI1788756, Length=453, Percent_Identity=38.6313465783664, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI1789193, Length=519, Percent_Identity=32.5626204238921, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1787994, Length=412, Percent_Identity=27.1844660194175, Blast_Score=97, Evalue=6e-21,
Organism=Escherichia coli, GI1786951, Length=139, Percent_Identity=26.6187050359712, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 95459; Mature: 95327

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00369 PTS_HPR_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILL
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEE
GDECQVTIEGEDEQAAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAANSSTTENSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGESCATAI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADEL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCC
TPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGE
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCCEEECCC
LGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEG
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCEEEEECC
AFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVS
CHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCE
YLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVK
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
DQLAEVKQSLRINHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAV
HHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCHHH
SMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHH
TLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEH
EECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHH
IEQSTISVAKLAQPVNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAGTAQQIETLLKHELEL
CCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILL
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEE
GDECQVTIEGEDEQAAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAANSSTTENSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIIAGESCATAI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADEL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCC
TPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGE
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCCEEECCC
LGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEG
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCEEEEECC
AFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVS
CHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCE
YLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVK
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
DQLAEVKQSLRINHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAV
HHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCHHH
SMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHH
TLGVDWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEH
EECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHH
IEQSTISVAKLAQPVNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAGTAQQIETLLKHELEL
CCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]