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The map label for this gene is nfo

Identifier: 153949100

GI number: 153949100

Start: 3028876

End: 3029733

Strand: Direct

Name: nfo

Synonym: YpsIP31758_2675

Alternate gene names: 153949100

Gene position: 3028876-3029733 (Clockwise)

Preceding gene: 153949961

Following gene: 153948478

Centisome position: 64.13

GC content: 49.07

Gene sequence:

>858_bases
ATGAAATTTGTCGGTGCACATGTCAGCGCAGCGGGTGGTGTAGATCAAGCGGTAATTAGAGCGCATGAACTTGAGGCCAC
GGCCTTTGCGCTGTTTACCAAGAATCAACGTCAATGGCGGGCCGCCCCTTTAGCGGAAGACGTAATTGAGAAATTTAAAC
TCACGTGTGAGAAGTACGGCTATACCTCGGCACAAATTCTGCCTCACGATAGTTACCTGATTAATCTCGGGCACCCAGTC
ACCGAGGCACTGGAAAAATCCCGTGAAGCCTTTATCGATGAGTTAGTTCGCTGCCAGCAACTGGGGTTATCATTACTGAA
CTTCCATCCCGGTAGCCATTTACTGCAAATTGATGAAGACCAATGCTTGGCGCGGATTGCCGAATCGATCAACATCGCGT
TAGACGCCACTGAAGGCGTGACTGCAGTAATTGAAAACACCGCAGGTCAGGGCAGTAACCTGGGCTTTAAGTTTGAACAT
TTAGCCGCCATCATTGAAAAAGTGGAAGATAAAAGCCGCGTCGGCGTCTGTATTGATACCTGCCATGCTTTCGCCGCTGG
CTATGATTTACGGACTGAAGAAGATTGTGAGCACACCTTCGCGGCATTGGGCAAGATCGTCGGCTTCCAGTATCTGCGTG
GGATGCATCTTAATGATGCGAAAAGCGAATTTAACAGCCGGGTTGACCGCCACCACAGCCTGGGTGAAGGCAATATTGGC
AAAACCGTATTCAGCTATATTATGCGCGACTCACGTTTCGATAATATCCCATTGATTCTGGAAACGGTGAATATGGATAT
CTGGGCCGAAGAGATCGCCTGGCTGAAATCACAGACAGAGATTGAGCCCTCGTTGTAA

Upstream 100 bases:

>100_bases
TAAGGGCCGTATCCGCGGCCCTTATTTCCCTTCACCGCTTATCCCATTCAATCATGTCCCTATCAATGCCATAATGGCCC
GGTTATCAAAGGAGAATGGA

Downstream 100 bases:

>100_bases
CGTAGAAAATGCCCGCCAGTAAACCTGGCGGGAATTGAACCTGACGGGAATTGAACCTAACGGGAATTGTGTCATCGCGG
ATGCCTTTAATGGCATACTC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLTCEKYGYTSAQILPHDSYLINLGHPV
TEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEH
LAAIIEKVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQTEIEPSL

Sequences:

>Translated_285_residues
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLTCEKYGYTSAQILPHDSYLINLGHPV
TEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEH
LAAIIEKVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQTEIEPSL
>Mature_285_residues
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLTCEKYGYTSAQILPHDSYLINLGHPV
TEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEH
LAAIIEKVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQTEIEPSL

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=278, Percent_Identity=80.9352517985612, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=46.360153256705, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322735, Length=284, Percent_Identity=41.5492957746479, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_YERP3 (A7FK62)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001401640.1
- ProteinModelPortal:   A7FK62
- SMR:   A7FK62
- STRING:   A7FK62
- GeneID:   5386421
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_2675
- NMPDR:   fig|349747.3.peg.4027
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   YPSE349747:YPSIP31758_2675-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31704; Mature: 31704

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLTCEKYG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHCC
YTSAQILPHDSYLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDED
CCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECHH
QCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEHLAAIIEKVEDKSRVGVCIDT
HHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
CHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQTEIEPSL
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLTCEKYG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHCC
YTSAQILPHDSYLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDED
CCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECHH
QCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEHLAAIIEKVEDKSRVGVCIDT
HHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
CHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQTEIEPSL
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA