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The map label for this gene is eptB [H]

Identifier: 153949028

GI number: 153949028

Start: 4601468

End: 4603159

Strand: Reverse

Name: eptB [H]

Synonym: YpsIP31758_4081

Alternate gene names: 153949028

Gene position: 4603159-4601468 (Counterclockwise)

Preceding gene: 153947475

Following gene: 153950690

Centisome position: 97.46

GC content: 44.33

Gene sequence:

>1692_bases
ATGGACAGAGTGAAATTTTTATCACAGCAAAGTCTTTCTTTGTTGTTGGCTGTTTATATTGGTATTTTCCTGAATATATC
TGTCTTTTATCGTCGTTTTGACTCATTCAGTCAGGGTATCCAAGGGATAAAAGTGGTATCAGCAATCACTGAAGTGATCG
CGATTATTCTGTTTACCTTTTTTGTTATGCGATTGATCTCACTTGGTGGAAGGTTTTTTTACCGAGTGGTTGCCTCGTTG
CTGGTGCTTATCTCTGTTGCTGCCAGTTATTACATGACATTTTTCAATGTGGTGATTGGTTACGGCATCGTTATTTCAGT
ATTGACCACAGATACCGATCTTTCTAAGGAAGTTATCGGGCTGCATTTTATTATTTGGATGGTGTTGGTCAGTGCATTGC
CTCTACTGCTTATCTGGAAGAATAGCCTGCGGTTAACATTGCTGGAGCAATGTTTAACGCCTGGGCAACGTATTAAACCC
GTATTGTTGATGGTGGCTGTTGTGGCGCTGGTTTGGCTGCCGTTGCGCTATCTGGATAAAGCTCAAGCTCTGGACGAACA
GCTAAAAACGGTGGATTTACCCAGTTATGGTGGGGTGGTTGCTCACTCTTATTTGCCATCTAACTGGTTGGCCGCTTTAG
GTTTGTACGCTTATACCCAATATAATGAGAAGTATGACGCGGCGGTATTGTATAACCCCGCTGAACATCATACTTATGTT
GCCCCCGCAGGTATTGATGATACCTATGTCGTGTTTATTATCGGCGAAACGACCCGCTGGGACCACATGGGGTTGTTGGG
ATACTCACGTGATACTACCCCTCGTCTGAGTAAAGAAAAGAACTTAGCTGCTTTCCGTGGTGAGTCCTGTGATACCTCAA
CCAAACTTTCTCTACGCTGCATGTTTGTCCGTGAAGGTGGCGTTGAAGATAACGCACAACGCACACTGAAAGAACAAAAC
GTTTTTGCTGTCATGAAATCGTTGGGATTCACATCCGAGTTGTTCGCCATGCAGAGTGAAATGTGGTTCTACAACAACGT
GAACACCAATAATTACTCATTTCGTGAGCTGATTGCATCAGAGAAACGTAACGATGGAAAACCGGTAGATGACATGTTGC
TGGTTGATGAATTAAAAGAGTCGTTAGGGCGTTATCCGGTGGGTAAACATTTAGTGGTGCTCCATACCAAAGGCTCACAT
TATCTTTATTCCCAACGCTACCCTCGCAGCTATGCCCGTTATCAACCGGAATGTATGGGAGTTGATGAGTTTTGCAGTAA
ACAGCAGTTGATTAATGCTTTTGATAACAGCGTGTTGTACACAGATTCGTTTATTGCCAATGTGATTGACCAAATGAGGG
ATAAAAAAGCGTTGGTATTCTATGCTGCCGATCATGGTGAATCTATCAGTGATAACTCTCACTTCCATGGTACTCCGCGT
GAAATGGCACCTCCGGAACAATTCCGAGTGCCATTGATGGTCTGGGCATCAGATAAATTCCTGGCACAGCCAGAGCATCT
TGCTGCTTTTGAACAACTACAGGTAGAACAGCGGGTAGGTAAAACACATCGCCACGCAGAACTCTTTGATTCTGTTTTGG
GGTGCATGGGCTATACCTCACCGGATGGTGGGATTGATGCTAAAAATAACTGGTGCCAGGCCCCTGATCGCCATCTTACA
CCCGCAATTTAG

Upstream 100 bases:

>100_bases
AATTCGCCGCGTGCTAACTGGCCCCATACTGAACCTGAAGGGGCGTTCACTGTCTGATTGATTCAAGCCGTAAATAGTGT
CTTTGGAGACCTAGCGTTAT

Downstream 100 bases:

>100_bases
CCGTAACTGGCCAGGCTTACCTAAGTCTGGCCCAATTTTTGATGTGCCTCCTCATTGTTGTTTCACGGCTAAGGTATACT
TTCAAATCCGATCCCCCTCC

Product: phosphoethanolamine transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MDRVKFLSQQSLSLLLAVYIGIFLNISVFYRRFDSFSQGIQGIKVVSAITEVIAIILFTFFVMRLISLGGRFFYRVVASL
LVLISVAASYYMTFFNVVIGYGIVISVLTTDTDLSKEVIGLHFIIWMVLVSALPLLLIWKNSLRLTLLEQCLTPGQRIKP
VLLMVAVVALVWLPLRYLDKAQALDEQLKTVDLPSYGGVVAHSYLPSNWLAALGLYAYTQYNEKYDAAVLYNPAEHHTYV
APAGIDDTYVVFIIGETTRWDHMGLLGYSRDTTPRLSKEKNLAAFRGESCDTSTKLSLRCMFVREGGVEDNAQRTLKEQN
VFAVMKSLGFTSELFAMQSEMWFYNNVNTNNYSFRELIASEKRNDGKPVDDMLLVDELKESLGRYPVGKHLVVLHTKGSH
YLYSQRYPRSYARYQPECMGVDEFCSKQQLINAFDNSVLYTDSFIANVIDQMRDKKALVFYAADHGESISDNSHFHGTPR
EMAPPEQFRVPLMVWASDKFLAQPEHLAAFEQLQVEQRVGKTHRHAELFDSVLGCMGYTSPDGGIDAKNNWCQAPDRHLT
PAI

Sequences:

>Translated_563_residues
MDRVKFLSQQSLSLLLAVYIGIFLNISVFYRRFDSFSQGIQGIKVVSAITEVIAIILFTFFVMRLISLGGRFFYRVVASL
LVLISVAASYYMTFFNVVIGYGIVISVLTTDTDLSKEVIGLHFIIWMVLVSALPLLLIWKNSLRLTLLEQCLTPGQRIKP
VLLMVAVVALVWLPLRYLDKAQALDEQLKTVDLPSYGGVVAHSYLPSNWLAALGLYAYTQYNEKYDAAVLYNPAEHHTYV
APAGIDDTYVVFIIGETTRWDHMGLLGYSRDTTPRLSKEKNLAAFRGESCDTSTKLSLRCMFVREGGVEDNAQRTLKEQN
VFAVMKSLGFTSELFAMQSEMWFYNNVNTNNYSFRELIASEKRNDGKPVDDMLLVDELKESLGRYPVGKHLVVLHTKGSH
YLYSQRYPRSYARYQPECMGVDEFCSKQQLINAFDNSVLYTDSFIANVIDQMRDKKALVFYAADHGESISDNSHFHGTPR
EMAPPEQFRVPLMVWASDKFLAQPEHLAAFEQLQVEQRVGKTHRHAELFDSVLGCMGYTSPDGGIDAKNNWCQAPDRHLT
PAI
>Mature_563_residues
MDRVKFLSQQSLSLLLAVYIGIFLNISVFYRRFDSFSQGIQGIKVVSAITEVIAIILFTFFVMRLISLGGRFFYRVVASL
LVLISVAASYYMTFFNVVIGYGIVISVLTTDTDLSKEVIGLHFIIWMVLVSALPLLLIWKNSLRLTLLEQCLTPGQRIKP
VLLMVAVVALVWLPLRYLDKAQALDEQLKTVDLPSYGGVVAHSYLPSNWLAALGLYAYTQYNEKYDAAVLYNPAEHHTYV
APAGIDDTYVVFIIGETTRWDHMGLLGYSRDTTPRLSKEKNLAAFRGESCDTSTKLSLRCMFVREGGVEDNAQRTLKEQN
VFAVMKSLGFTSELFAMQSEMWFYNNVNTNNYSFRELIASEKRNDGKPVDDMLLVDELKESLGRYPVGKHLVVLHTKGSH
YLYSQRYPRSYARYQPECMGVDEFCSKQQLINAFDNSVLYTDSFIANVIDQMRDKKALVFYAADHGESISDNSHFHGTPR
EMAPPEQFRVPLMVWASDKFLAQPEHLAAFEQLQVEQRVGKTHRHAELFDSVLGCMGYTSPDGGIDAKNNWCQAPDRHLT
PAI

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=555, Percent_Identity=63.6036036036036, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI87082372, Length=544, Percent_Identity=25.1838235294118, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082223, Length=460, Percent_Identity=25.2173913043478, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790392, Length=463, Percent_Identity=23.3261339092873, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 63789; Mature: 63789

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646 [H]