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The map label for this gene is glgX

Identifier: 153948572

GI number: 153948572

Start: 4495964

End: 4497952

Strand: Reverse

Name: glgX

Synonym: YpsIP31758_4005

Alternate gene names: 153948572

Gene position: 4497952-4495964 (Counterclockwise)

Preceding gene: 153947987

Following gene: 153947074

Centisome position: 95.23

GC content: 53.59

Gene sequence:

>1989_bases
ATGGCGGTATTAACCCATGGATCACCGACACCTTCTGGCGCCTACTTTGATGGGAAGGGCATTAATTTCACACTATTTTC
AGCCCATGCTGAGCAGGTGACGTTGTGTCTGTTTGATGAGCAGGGGCAAGAGCGGCAGATAGCCATGCCTGCTCGTACCG
GTGACATCTGGCATGGCTATCTTCCCGGCGGCAAGCCGGGGCAACGCTATGGTTACCGGGTCAGCGGGCCTTTCGACCCC
AGTCGAGGCCACCGTTTTAACCCACATAAGTTGTTGATAGACCCCCGAACTCGTGCGTTAGAAGGCAAGGTTGGTGATGA
TCCCCGTTTTACCGGTGGCGTTAGCCAGCCCGATGTCCGCGACAGTGCGGCGGCGTTACCTAAATGTCTTGTGATACACG
AGGAATATGACTGGCAGGGGGATAAACCGCCCGCCATTCCTTGGGGTAATACCGTGATTTATGAAGCCCATGTCCGTGGT
TTAACCCAATTACATCCTGATATTCCGCCAGAGCTGCGCGGTACCTATGCCGCCTTGGCACACCCTGCTCTGATTGAACA
TCTCAAAACATTGGGGATTACCACACTGGAGTTGCTGCCAGTGCAATTTCATATTGATGAACCCCGGCTACAAAAAATGG
GGCTGAGCAATTACTGGGGCTATAACGTGTTAGCGCCCTTTGCGGTGGATCCAGATTATGCCTCGGGCCGCGAGGGCATA
TCACCGCTGAGAGAACTACGGGATGCGATTAAAGCCCTGCATAACGCGGGCATTGAAGTCATTCTGGATGTGGTGTTCAA
CCACAGCGCTGAGTTGGATGTCTTCGGCCCGACCTTGTGTCAGCGTGGTATCGACAATGCCAGCTACTACTGGCTGACCC
CTGATGGTGAATACGACAACATCACGGGCTGCGGCAATGCACTGCGCCTCAGTCATCCGTATGTCACACAGTGGGTTATT
GATTGCCTAAATTATTGGCGCGATAGCTGCCATGTGGACGGCTTTCGTTTTGACTTAGGCACGGTACTGGGCCGCACACC
GGCGTTCGATCAACATGCGCCTTTATTTGCCGCCTTGGCGGCCGATGAGCGGCTCAGCGCCTGCAAATTGATTGCAGAAC
CTTGGGATATCGGTCTGGGCGGTTATCAATTGGGGAACTTCCCCACCGGCTTTAGCGAATGGAATGACCAGTACCGCGAT
GCCATGCGCGGTTTCTGGCTACGAGGGGAGGTGCCGCGCGGTACCTTTGCCCAGCATTTTGCTGCTTCAAGCCGCCTGTT
TGAACAGCGAGGGCGCTTACCCTCTGCCAGCATTAATCAAATTACCGCCCATGATGGTTTCACCTTGCTGGATTTGCTGT
GTTTTAACCAAAAACATAACCAAATGAATGGCGAAGAAAATCGTGATGGCAGTGATAACAACCATAGCAATAATTTTGGT
TGTGAAGGCTTGGTGGCTGACGCGGCTATCTGGCAACGGCGTAAAGCCTGTCAGCGGGCGCTACTGACCACCTTATTACT
CTCGCAGGGAACGCCGATGTTACTGGCCGGTGACGAACAGGGCCACAGCCAGCAAGGCAACAACAATGCTTATTGTCAGA
ACAACATCCTCACCTGGCTCGATTGGGGCAGTGCGGATCGTGCATTAATGACGTTTACCGCTGACCTTATCCGGCTGCGC
CAGCAAATCCCAGCCCTGACTCAGGACCAATGGTGGCAGAGCGGCGATAGTAATGTGCAATGGTTGGATAGTCAGGGACA
GGCGCTGAGTGATGCTGCTTGGGAGCAAGGCTGCCAGCAGCAACTACAGATTTTGTTATCTCAGCGCTGGTTGGTGCTCA
TCAATGCCACTGATCACGAGTGTGAAATGCACTTACCTGAAGGGGAATGGGAGGGGATTCCTCCCTTTGGGGTGTCAGAC
CATGCGGAGCGATTAACCACATGGCGCGGGTCCGCGCATACCATTTGTGTGCTCATAAAGAGAGATTAA

Upstream 100 bases:

>100_bases
GGCATTGATAGCCATCATGTGAGGAGCCACAACCACGAGCATTCGTTGTTACTCACCTTGCCACCGCTGGCAACGATTTA
CCTGCTGCGGGAGAACTAGG

Downstream 100 bases:

>100_bases
GGAGCTTTTATGGTGAGGTTTGAAAGTACAGACTCATTGATGTTGGCCAGACAATTACCCAATAAAACCGTGGCACTCAT
TCTGGCGGGAGGCCGTGGCT

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: Glycogen operon protein GlgX

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MAVLTHGSPTPSGAYFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGYLPGGKPGQRYGYRVSGPFDP
SRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVRDSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRG
LTQLHPDIPPELRGTYAALAHPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
SPLRELRDAIKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDNITGCGNALRLSHPYVTQWVI
DCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALAADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRD
AMRGFWLRGEVPRGTFAQHFAASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWLDWGSADRALMTFTADLIRLR
QQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQQLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSD
HAERLTTWRGSAHTICVLIKRD

Sequences:

>Translated_662_residues
MAVLTHGSPTPSGAYFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGYLPGGKPGQRYGYRVSGPFDP
SRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVRDSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRG
LTQLHPDIPPELRGTYAALAHPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
SPLRELRDAIKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDNITGCGNALRLSHPYVTQWVI
DCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALAADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRD
AMRGFWLRGEVPRGTFAQHFAASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWLDWGSADRALMTFTADLIRLR
QQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQQLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSD
HAERLTTWRGSAHTICVLIKRD
>Mature_661_residues
AVLTHGSPTPSGAYFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGYLPGGKPGQRYGYRVSGPFDPS
RGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVRDSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGL
TQLHPDIPPELRGTYAALAHPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGIS
PLRELRDAIKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDNITGCGNALRLSHPYVTQWVID
CLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALAADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDA
MRGFWLRGEVPRGTFAQHFAASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFGC
EGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWLDWGSADRALMTFTADLIRLRQ
QIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQQLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDH
AERLTTWRGSAHTICVLIKRD

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI2367229, Length=658, Percent_Identity=60.790273556231, Blast_Score=826, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGX_YERP3 (A7FNX4)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402952.1
- ProteinModelPortal:   A7FNX4
- SMR:   A7FNX4
- STRING:   A7FNX4
- GeneID:   5388030
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_4005
- NMPDR:   fig|349747.3.peg.2716
- eggNOG:   COG1523
- HOGENOM:   HBG350946
- OMA:   EMDAAAW
- ProtClustDB:   PRK03705
- BioCyc:   YPSE349747:YPSIP31758_4005-MONOMER
- HAMAP:   MF_01248
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR02100

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73939; Mature: 73807

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: ACT_SITE 338-338 ACT_SITE 373-373

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLTHGSPTPSGAYFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGY
CEEEECCCCCCCCCEECCCCCEEEEEECCCCEEEEEEECCCCCCCEEECCCCCCCCEECC
LPGGKPGQRYGYRVSGPFDPSRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVR
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCC
DSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGLTQLHPDIPPELRGTYAALA
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHCCHHHHCCCCCHHHCCHHHHHH
HPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
HHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECEECCCCCCCCCHHH
SPLRELRDAIKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDN
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCHHHHHHCCCCCEEEEECCCCCCCC
ITGCGNALRLSHPYVTQWVIDCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALA
CCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCHHEEHH
ADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDAMRGFWLRGEVPRGTFAQHF
HHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
AASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
HHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCC
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEE
DWGSADRALMTFTADLIRLRQQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQ
ECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
QLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDHAERLTTWRGSAHTICVLIK
HHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEE
RD
CC
>Mature Secondary Structure 
AVLTHGSPTPSGAYFDGKGINFTLFSAHAEQVTLCLFDEQGQERQIAMPARTGDIWHGY
EEEECCCCCCCCCEECCCCCEEEEEECCCCEEEEEEECCCCCCCEEECCCCCCCCEECC
LPGGKPGQRYGYRVSGPFDPSRGHRFNPHKLLIDPRTRALEGKVGDDPRFTGGVSQPDVR
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCC
DSAAALPKCLVIHEEYDWQGDKPPAIPWGNTVIYEAHVRGLTQLHPDIPPELRGTYAALA
HHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHCCHHHHCCCCCHHHCCHHHHHH
HPALIEHLKTLGITTLELLPVQFHIDEPRLQKMGLSNYWGYNVLAPFAVDPDYASGREGI
HHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECEECCCCCCCCCHHH
SPLRELRDAIKALHNAGIEVILDVVFNHSAELDVFGPTLCQRGIDNASYYWLTPDGEYDN
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCHHHHHHCCCCCEEEEECCCCCCCC
ITGCGNALRLSHPYVTQWVIDCLNYWRDSCHVDGFRFDLGTVLGRTPAFDQHAPLFAALA
CCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCHHEEHH
ADERLSACKLIAEPWDIGLGGYQLGNFPTGFSEWNDQYRDAMRGFWLRGEVPRGTFAQHF
HHHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
AASSRLFEQRGRLPSASINQITAHDGFTLLDLLCFNQKHNQMNGEENRDGSDNNHSNNFG
HHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCC
CEGLVADAAIWQRRKACQRALLTTLLLSQGTPMLLAGDEQGHSQQGNNNAYCQNNILTWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEE
DWGSADRALMTFTADLIRLRQQIPALTQDQWWQSGDSNVQWLDSQGQALSDAAWEQGCQQ
ECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
QLQILLSQRWLVLINATDHECEMHLPEGEWEGIPPFGVSDHAERLTTWRGSAHTICVLIK
HHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEE
RD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA