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The map label for this gene is menE [H]

Identifier: 153948224

GI number: 153948224

Start: 1728475

End: 1729890

Strand: Direct

Name: menE [H]

Synonym: YpsIP31758_1486

Alternate gene names: 153948224

Gene position: 1728475-1729890 (Clockwise)

Preceding gene: 153948854

Following gene: 153949586

Centisome position: 36.59

GC content: 57.42

Gene sequence:

>1416_bases
ATGGCATCGTTAGGCCATCCGCCTTGGGAGCATTGGCCTTGGCAGTATTGGGCCAATCTGCGGCCTCAAGCGCTCGCTAT
ACGTTCTGGGGGGCAACGGATCAGTTGGCAACAGTTAGCCAGTGATATCAATGCGTTGGCGGCTTCTTTTCAACAGCAAG
GTGTGGTACCGGGGAGCGGCGTGGCTTTGCGCGGTAAAAACAGTTATACCCTGCTATTGGCTTATCTGGCGGCATTGCAG
TGTGCAGCTCGCGTTTTACCGCTGAATCCGCAATTGCCGGATGCGCTTTTGGCGCAGCGCCTGCCAACGCTGGATATTCA
CTTTATGCTGAATTTAGCCACCCCGTTGCCCCCGCATTTTGCCTTTACTCCGTTGGTTCCCCGCACCGTTGATTCGGCAG
CCGTGACCCCGATAGCGTGGGATGGCCAACGGCTGGCGACGCTGACGCTAACCTCAGGTTCCTCCGGCTTACCGAAGGCC
GCAGTACACTCGCTGGGCGCACATCGGGCTAATGCCGACGGCGTGCTGGCGCTGATGAATTTCACCGCCAGTGATAGCTG
GTTGCTGTCGTTACCGTTGTTTCATGTCTCAGGGCAGGGGATTGTCTGGCGTTGGCTGACGGCGGGGGCGACACTGGGGG
TACAAGAGGGCGTGCCACTGGTTGAGGCTTTGGCGGGGTGCAGCCATGCGTCATTAGTTCCCACGCAATTATGGCGTTTG
TTGGCGAATGCCGACACCCCCTTGACGTTGAAAGAGGTACTGTTGGGGGGCGCGATGATCCCAACCGAACTGACCGAACA
GGCCGAGGCCCACGGCATCCGCTGTTGGTGTGGCTACGGCTTGACGGAGTTGGCATCAACGGTCTGTGCCAAGCGGGCAG
ACGGGCGACCTGGTGTCGGTGTCGCGCTCGCAGGCCGTCAGGTGAAACTGGTAGACGATGAAGTCTGGATAAAAGCCGAT
TGTCTGGCTGCCGGTTACTGGCAACAGGGGCAATTACTGCCACTCACGGACAGCGACGGGTGGTTCCATACCCGAGATCG
CGGTAGGTGGCAGCAAGGCGAATTATGTATTCTCGGGCGGTTGGATAATCTGTTTTTCAGTGGTGGCGAGGGGATCCAGC
CAGAAGACATCGAGCGAATTTTATTGCAGTATTCAGGGGTGCAGCACGCTTTTGTTATTCCTGTTGCTGACGCTGAATTC
GGTCACCGGCCAGTGGCGGTCATTGATTCCGCTGATGAGGTGGATGTCGTGGCTTTAGCTGATTGGTTAGCCCCACGATT
GGCCGTCTTCCAGCGGCCCATTGCTTTTTACCGGCTACCCACATTTTTGAAGCGTGGCGGGATTAAGGTCTCAAGGCAGC
AACTTGCCGACTGGCTGCGCGGCAAGTATGAAGGGGAGAAGATTGGCGAAAGATAG

Upstream 100 bases:

>100_bases
TTCCGGGGTTGGATACGCTGGACCTGATGCAGGCGCAAGTCGTGCGGCCGTGGCCAGACAGCCCGTTGCCGCTAATCACG
ACCGAGCAGTTGGGCGTGGT

Downstream 100 bases:

>100_bases
AGCATGTCAGGCAAACTCAGCCCAGTTTATTGTTAACGAGAGACCCAGAGGGGAAAGGCCGCACCATGGATTACAGTAAA
ATTATCAAAGAGGTTGGGCG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAASFQQQGVVPGSGVALRGKNSYTLLLAYLAALQ
CAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLVPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKA
AVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVQEGVPLVEALAGCSHASLVPTQLWRL
LANADTPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKAD
CLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERILLQYSGVQHAFVIPVADAEF
GHRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKVSRQQLADWLRGKYEGEKIGER

Sequences:

>Translated_471_residues
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAASFQQQGVVPGSGVALRGKNSYTLLLAYLAALQ
CAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLVPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKA
AVHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVQEGVPLVEALAGCSHASLVPTQLWRL
LANADTPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKAD
CLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERILLQYSGVQHAFVIPVADAEF
GHRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKVSRQQLADWLRGKYEGEKIGER
>Mature_470_residues
ASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAASFQQQGVVPGSGVALRGKNSYTLLLAYLAALQC
AARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHFAFTPLVPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAA
VHSLGAHRANADGVLALMNFTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVQEGVPLVEALAGCSHASLVPTQLWRLL
ANADTPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVGVALAGRQVKLVDDEVWIKADC
LAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGRLDNLFFSGGEGIQPEDIERILLQYSGVQHAFVIPVADAEFG
HRPVAVIDSADEVDVVALADWLAPRLAVFQRPIAFYRLPTFLKRGGIKVSRQQLADWLRGKYEGEKIGER

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=54.7671840354767, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI145693145, Length=451, Percent_Identity=24.1685144124169, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788107, Length=362, Percent_Identity=24.3093922651934, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786801, Length=438, Percent_Identity=25.3424657534247, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=345, Percent_Identity=30.7246376811594, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=450, Percent_Identity=22.4444444444444, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17510401, Length=324, Percent_Identity=28.0864197530864, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51180; Mature: 51049

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAASFQQQGVVPGSG
CCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHF
EEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCC
AFTPLVPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMN
CCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
FTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVQEGVPLVEALAGCSHASLVPTQLWRL
ECCCCCEEEEEEEEEECCCCEEEEEECCCCEECHHCCCHHHHHHHCCCCCCCCHHHHHHH
LANADTPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVG
HHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCE
VALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGR
EEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCEEEEEE
LDNLFFSGGEGIQPEDIERILLQYSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALA
CCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCHHHHH
DWLAPRLAVFQRPIAFYRLPTFLKRGGIKVSRQQLADWLRGKYEGEKIGER
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
ASLGHPPWEHWPWQYWANLRPQALAIRSGGQRISWQQLASDINALAASFQQQGVVPGSG
CCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VALRGKNSYTLLLAYLAALQCAARVLPLNPQLPDALLAQRLPTLDIHFMLNLATPLPPHF
EEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCC
AFTPLVPRTVDSAAVTPIAWDGQRLATLTLTSGSSGLPKAAVHSLGAHRANADGVLALMN
CCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEE
FTASDSWLLSLPLFHVSGQGIVWRWLTAGATLGVQEGVPLVEALAGCSHASLVPTQLWRL
ECCCCCEEEEEEEEEECCCCEEEEEECCCCEECHHCCCHHHHHHHCCCCCCCCHHHHHHH
LANADTPLTLKEVLLGGAMIPTELTEQAEAHGIRCWCGYGLTELASTVCAKRADGRPGVG
HHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCCE
VALAGRQVKLVDDEVWIKADCLAAGYWQQGQLLPLTDSDGWFHTRDRGRWQQGELCILGR
EEECCCEEEEECCCEEEEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCCCCEEEEEE
LDNLFFSGGEGIQPEDIERILLQYSGVQHAFVIPVADAEFGHRPVAVIDSADEVDVVALA
CCHHEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCHHHHH
DWLAPRLAVFQRPIAFYRLPTFLKRGGIKVSRQQLADWLRGKYEGEKIGER
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEECHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]