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The map label for this gene is hydN [H]

Identifier: 153947862

GI number: 153947862

Start: 4153449

End: 4154006

Strand: Reverse

Name: hydN [H]

Synonym: YpsIP31758_3683

Alternate gene names: 153947862

Gene position: 4154006-4153449 (Counterclockwise)

Preceding gene: 153948456

Following gene: 153947573

Centisome position: 87.95

GC content: 56.45

Gene sequence:

>558_bases
ATGAACCGTTTTGTTATCGCTGATATGACTCAGTGCATTGGCTGCCGGACCTGTGAAATCGCCTGTGTGGTGGCGCATCG
CGCAGATAATAAGGTCAGTACCCTTATGCCGGAGCAGTTTCACCCACGCCTGAGCGTCATGAAAAGCTTTGCGGTCAGCA
CACCGATACTCTGCCATCAATGTGAAGATGCGCCGTGCGAAAACGCCTGCCCAAACGGGGCGATTATCACGGGGTCCCAT
GGTGTGCAACTGCTGGCGTCGCGGTGTATCGGCTGTAAAACCTGCATGTTGGTGTGCCCGTTTGGTGCCATGAGCATTAT
TGAGCGGCCAGCCGATGGTCAACAGGCGGCAGACAGTCAACCGGTGATGGCGAGTGGATCAGGGCGAGCACAGGCGCATA
AATGCGATTTATGCCATCAGCGGGAATCCGGCCCAGCTTGTATTGAGGTCTGCCCGACTAATGCATTGAAACTGGTGACG
CCAGGGGTATTGGAAGCCCTACAGCGCGAAAAACAGTTGCGGGCCGCACGAGGCAGCGCACCGGGTATCGCCCGTTAA

Upstream 100 bases:

>100_bases
GCTGACTTGATTGTGACCGCCATCGCGGAGGGGCGCAAAGCGGCAGAGAGTATTGCGTATTATTTGAACGTTTTATAACG
CCTGCGGCCAGGAGAGAAAG

Downstream 100 bases:

>100_bases
CCTACGATCATAAGGATAAAAAATGAAAAAGATCCTCACGGTCTGCCCCTATTGCGGCTCGGGATGTAAGTTAAATTTAT
TGGTCGAAGACGGCGAGATT

Product: iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MNRFVIADMTQCIGCRTCEIACVVAHRADNKVSTLMPEQFHPRLSVMKSFAVSTPILCHQCEDAPCENACPNGAIITGSH
GVQLLASRCIGCKTCMLVCPFGAMSIIERPADGQQAADSQPVMASGSGRAQAHKCDLCHQRESGPACIEVCPTNALKLVT
PGVLEALQREKQLRAARGSAPGIAR

Sequences:

>Translated_185_residues
MNRFVIADMTQCIGCRTCEIACVVAHRADNKVSTLMPEQFHPRLSVMKSFAVSTPILCHQCEDAPCENACPNGAIITGSH
GVQLLASRCIGCKTCMLVCPFGAMSIIERPADGQQAADSQPVMASGSGRAQAHKCDLCHQRESGPACIEVCPTNALKLVT
PGVLEALQREKQLRAARGSAPGIAR
>Mature_185_residues
MNRFVIADMTQCIGCRTCEIACVVAHRADNKVSTLMPEQFHPRLSVMKSFAVSTPILCHQCEDAPCENACPNGAIITGSH
GVQLLASRCIGCKTCMLVCPFGAMSIIERPADGQQAADSQPVMASGSGRAQAHKCDLCHQRESGPACIEVCPTNALKLVT
PGVLEALQREKQLRAARGSAPGIAR

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=176, Percent_Identity=50, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI1788811, Length=177, Percent_Identity=46.3276836158192, Blast_Score=168, Evalue=2e-43,
Organism=Escherichia coli, GI2367245, Length=171, Percent_Identity=46.1988304093567, Blast_Score=158, Evalue=2e-40,
Organism=Escherichia coli, GI87082179, Length=182, Percent_Identity=44.5054945054945, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI87082180, Length=186, Percent_Identity=39.7849462365591, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI87082114, Length=200, Percent_Identity=39.5, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI1789079, Length=187, Percent_Identity=42.2459893048128, Blast_Score=123, Evalue=7e-30,
Organism=Escherichia coli, GI1787122, Length=195, Percent_Identity=27.1794871794872, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787872, Length=188, Percent_Identity=26.5957446808511, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI2367345, Length=199, Percent_Identity=29.6482412060301, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19662; Mature: 19662

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
12.4 %Cys+Met (Translated Protein)
8.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
12.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVIADMTQCIGCRTCEIACVVAHRADNKVSTLMPEQFHPRLSVMKSFAVSTPILCHQ
CCCEEEECHHHHHCCCCHHEEEEEEECCCCCHHHHCCHHHCHHHHHHHHHHHCCCHHEEC
CEDAPCENACPNGAIITGSHGVQLLASRCIGCKTCMLVCPFGAMSIIERPADGQQAADSQ
CCCCCCCCCCCCCCEEECCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCC
PVMASGSGRAQAHKCDLCHQRESGPACIEVCPTNALKLVTPGVLEALQREKQLRAARGSA
CEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEHCCHHHHHHHHHHHHHHHHCCCC
PGIAR
CCCCC
>Mature Secondary Structure
MNRFVIADMTQCIGCRTCEIACVVAHRADNKVSTLMPEQFHPRLSVMKSFAVSTPILCHQ
CCCEEEECHHHHHCCCCHHEEEEEEECCCCCHHHHCCHHHCHHHHHHHHHHHCCCHHEEC
CEDAPCENACPNGAIITGSHGVQLLASRCIGCKTCMLVCPFGAMSIIERPADGQQAADSQ
CCCCCCCCCCCCCCEEECCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCC
PVMASGSGRAQAHKCDLCHQRESGPACIEVCPTNALKLVTPGVLEALQREKQLRAARGSA
CEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEHCCHHHHHHHHHHHHHHHHCCCC
PGIAR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]