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The map label for this gene is fdhF [H]

Identifier: 153947573

GI number: 153947573

Start: 4151279

End: 4153426

Strand: Reverse

Name: fdhF [H]

Synonym: YpsIP31758_3682

Alternate gene names: 153947573

Gene position: 4153426-4151279 (Counterclockwise)

Preceding gene: 153947862

Following gene: 153949225

Centisome position: 87.93

GC content: 53.35

Gene sequence:

>2148_bases
ATGAAAAAGATCCTCACGGTCTGCCCCTATTGCGGCTCGGGATGTAAGTTAAATTTATTGGTCGAAGACGGCGAGATTGT
CGGTGCCGAACCCGCTGATGGTGTCACTAATCAGGGAGCGTTGTGTCTGAAAGGGTACTACGGCTGGGATTTTCTTAACG
ATACCCGCTTGTTGACGCCACGGTTGACCACGCCCTTATTGCGGCGTCAGCGAGGCGAGGCGTTTCAGCCCGTGAGTTGG
GAGGCGGCGATTGCGTTTATCACGGCTAAACTTAGCGCCATAAAAGAACGTCATGGGCCTGATGCCATCATGATGACGGG
CTCTTCCCGTGGGCCAGGTAACGAAGCCAATTATGTGATGCAAAAATTTGCTCGTGCGGCGATCGGTACCAACAATATTG
ATTGCTGCGCCCGCCTGTGCCATGGCCCGTCAGTGGCTGGATTGCAGCAAACGCTGGGTAACGGCGCGATGAGCAACTCT
ATTGGTGAAATTGAGAATACTGACTGCATTTTGGTCTTTGGTTATAACGCGGCGGATTCTCACCCCATTGTTGCCAGACG
TATTCTGAAAGCCAAAAATAAGGGGGCTAAACTTATTGTCTGTGATCCACGGCATATCGAAACCGCCCGTATCGCCGATC
AATGGTTGCCGCTGAAAAATGGTGCCAACATGGCACTGGTCAATGCATTTATTCATGTTCTGATTGAAGAAAAACTCTAC
GACGCAAATTATGTCCGCGACCATACCCGTGGCTTTGATGAACTGAAAGCAGGCGTTGCCGCATACCGCCCAGAGGATGT
TGAACCGATTACGGGGCTGTCTGCCAAAGCGGTGCGTCAGGCCATGCGCACTTATGCTGCGGCCCCTGCGGCCACCCTTC
TATGGGGGATGGGCGTGACCCAATGGGGCCAGGCGGTTGATGTGGTGAAAGGGCTCTCTTCGTTAGCGTTATTAACCGGT
AATCTGGGGCGGCCAAATGTGGGCGTAGGGCCAGTACGAGGTCAGAATAATGTGCAGGGCGCGTGCGATATGGGCGTGTT
GCCGAATACCTTCCCTGGCTATGAAAAAGTGACCGACAGTGAGGCCAGAGCGCGTTTTGCTCACGCCTGGGGGGTCGCTG
AACTGCCCGCGCATGTTGGGTATTCGATTACTGATGTGCCGCATAAAGTGGCAGAAGGGAAGCTTAAGGCCTACTACGTA
TTTGGTGAAGATCCAATCCAGACCGAGCCAGATTTAGCGGCGATGCGTGCGGCTTTTGCCGCACTGGATCTGGTCGTGGT
GCAGGATATCTTTATGACCAAAACGGCAGAACTGGCGGATGTCGTACTGCCTGCAACATCCTGGGGGGAGCATGAAGGGG
TCTATACCAGTGCTGACCGGGGGTTCCAGCACTTTAATAAAGCCATTGAACCGAAAGGGGATGTGAAACCGGACTGGGAG
ATTATCAGCCTGATATCAACCGCCATGGGTTACCCGATGGCATACCAAAATACGCAACAAATCTGGGATGAGATGCGTGG
CCTGTGCCCGCCATTCGCTGGGGCCACCTATGATAAACTGGCAGGATTGGGCTACGTGCAATGGCCTTGCCCAACCGAAG
ATCACCCTGGAACACCTTATCTCTATACCGATAACCAATTCGCGACGGCTGACGGCAAAGGTTTGCTCAGTTGCGCGCCG
TGGCAGCCGCCTCATGAGCAGACCGACAGTGATTATCCTCTGGCCCTCTGTACCGTGCGTGAAGTGGGGCATTACAGTTG
CCGCTCAATGACCGGTAACTGCGCCACGCTAAAAATCTTGGCGGATGAGCCGGGCTATGTGCAAATCCATCCTGAGGATG
CCAGCTATTTGGGGGTTATCGATCAGGCGCTGATCTGGATTTCATCACGACGCGGGAAAGTGATCGCCCGTGCCAGTGTG
ACCGAACGGGCTAATCGTGGCGCGGTGTATATGACCTATCAGTGGTGGATTGGGGCCTGTAATGAACTGACTGTGGACCA
TGTTGATCCCGTGTCAAAAACACCGGAATTTAAACATTGTGCGGTTAAGGTCGAGGCGATTAGCGATCAAACTGCGGCAG
AGCAATATGTTGAGCAACAATACAGCCAGTTGAAAAAACGGTTATCAGACGCCGCAGTTGTACCATGA

Upstream 100 bases:

>100_bases
CCAGGGGTATTGGAAGCCCTACAGCGCGAAAAACAGTTGCGGGCCGCACGAGGCAGCGCACCGGGTATCGCCCGTTAACC
TACGATCATAAGGATAAAAA

Downstream 100 bases:

>100_bases
CTTGTCGTGATTGAGCGTTGATTATGCCACGGCGAGACCTACTCGCCGTGGCGACAGCTATTTATTTTGTAACCGCGGGG
GTGTTTTGTAAATGCTGTAA

Product: anaerobic formate dehydrogenase, alpha subunit, -type

Products: NA

Alternate protein names: Formate dehydrogenase-H subunit alpha; FDH-H; Formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MKKILTVCPYCGSGCKLNLLVEDGEIVGAEPADGVTNQGALCLKGYYGWDFLNDTRLLTPRLTTPLLRRQRGEAFQPVSW
EAAIAFITAKLSAIKERHGPDAIMMTGSSRGPGNEANYVMQKFARAAIGTNNIDCCARLCHGPSVAGLQQTLGNGAMSNS
IGEIENTDCILVFGYNAADSHPIVARRILKAKNKGAKLIVCDPRHIETARIADQWLPLKNGANMALVNAFIHVLIEEKLY
DANYVRDHTRGFDELKAGVAAYRPEDVEPITGLSAKAVRQAMRTYAAAPAATLLWGMGVTQWGQAVDVVKGLSSLALLTG
NLGRPNVGVGPVRGQNNVQGACDMGVLPNTFPGYEKVTDSEARARFAHAWGVAELPAHVGYSITDVPHKVAEGKLKAYYV
FGEDPIQTEPDLAAMRAAFAALDLVVVQDIFMTKTAELADVVLPATSWGEHEGVYTSADRGFQHFNKAIEPKGDVKPDWE
IISLISTAMGYPMAYQNTQQIWDEMRGLCPPFAGATYDKLAGLGYVQWPCPTEDHPGTPYLYTDNQFATADGKGLLSCAP
WQPPHEQTDSDYPLALCTVREVGHYSCRSMTGNCATLKILADEPGYVQIHPEDASYLGVIDQALIWISSRRGKVIARASV
TERANRGAVYMTYQWWIGACNELTVDHVDPVSKTPEFKHCAVKVEAISDQTAAEQYVEQQYSQLKKRLSDAAVVP

Sequences:

>Translated_715_residues
MKKILTVCPYCGSGCKLNLLVEDGEIVGAEPADGVTNQGALCLKGYYGWDFLNDTRLLTPRLTTPLLRRQRGEAFQPVSW
EAAIAFITAKLSAIKERHGPDAIMMTGSSRGPGNEANYVMQKFARAAIGTNNIDCCARLCHGPSVAGLQQTLGNGAMSNS
IGEIENTDCILVFGYNAADSHPIVARRILKAKNKGAKLIVCDPRHIETARIADQWLPLKNGANMALVNAFIHVLIEEKLY
DANYVRDHTRGFDELKAGVAAYRPEDVEPITGLSAKAVRQAMRTYAAAPAATLLWGMGVTQWGQAVDVVKGLSSLALLTG
NLGRPNVGVGPVRGQNNVQGACDMGVLPNTFPGYEKVTDSEARARFAHAWGVAELPAHVGYSITDVPHKVAEGKLKAYYV
FGEDPIQTEPDLAAMRAAFAALDLVVVQDIFMTKTAELADVVLPATSWGEHEGVYTSADRGFQHFNKAIEPKGDVKPDWE
IISLISTAMGYPMAYQNTQQIWDEMRGLCPPFAGATYDKLAGLGYVQWPCPTEDHPGTPYLYTDNQFATADGKGLLSCAP
WQPPHEQTDSDYPLALCTVREVGHYSCRSMTGNCATLKILADEPGYVQIHPEDASYLGVIDQALIWISSRRGKVIARASV
TERANRGAVYMTYQWWIGACNELTVDHVDPVSKTPEFKHCAVKVEAISDQTAAEQYVEQQYSQLKKRLSDAAVVP
>Mature_715_residues
MKKILTVCPYCGSGCKLNLLVEDGEIVGAEPADGVTNQGALCLKGYYGWDFLNDTRLLTPRLTTPLLRRQRGEAFQPVSW
EAAIAFITAKLSAIKERHGPDAIMMTGSSRGPGNEANYVMQKFARAAIGTNNIDCCARLCHGPSVAGLQQTLGNGAMSNS
IGEIENTDCILVFGYNAADSHPIVARRILKAKNKGAKLIVCDPRHIETARIADQWLPLKNGANMALVNAFIHVLIEEKLY
DANYVRDHTRGFDELKAGVAAYRPEDVEPITGLSAKAVRQAMRTYAAAPAATLLWGMGVTQWGQAVDVVKGLSSLALLTG
NLGRPNVGVGPVRGQNNVQGACDMGVLPNTFPGYEKVTDSEARARFAHAWGVAELPAHVGYSITDVPHKVAEGKLKAYYV
FGEDPIQTEPDLAAMRAAFAALDLVVVQDIFMTKTAELADVVLPATSWGEHEGVYTSADRGFQHFNKAIEPKGDVKPDWE
IISLISTAMGYPMAYQNTQQIWDEMRGLCPPFAGATYDKLAGLGYVQWPCPTEDHPGTPYLYTDNQFATADGKGLLSCAP
WQPPHEQTDSDYPLALCTVREVGHYSCRSMTGNCATLKILADEPGYVQIHPEDASYLGVIDQALIWISSRRGKVIARASV
TERANRGAVYMTYQWWIGACNELTVDHVDPVSKTPEFKHCAVKVEAISDQTAAEQYVEQQYSQLKKRLSDAAVVP

Specific function: Decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=715, Percent_Identity=63.0769230769231, Blast_Score=990, Evalue=0.0,
Organism=Escherichia coli, GI1788534, Length=789, Percent_Identity=24.4613434727503, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI3868719, Length=621, Percent_Identity=26.8921095008052, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI3868720, Length=624, Percent_Identity=27.724358974359, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1787778, Length=701, Percent_Identity=24.3937232524964, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI87081797, Length=804, Percent_Identity=24.5024875621891, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1787870, Length=786, Percent_Identity=24.3002544529262, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI171474008, Length=787, Percent_Identity=24.0152477763659, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI145693196, Length=650, Percent_Identity=21.3846153846154, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787231, Length=712, Percent_Identity=21.6292134831461, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006478
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 77871; Mature: 77871

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILTVCPYCGSGCKLNLLVEDGEIVGAEPADGVTNQGALCLKGYYGWDFLNDTRLLTP
CCCCEEECCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCEEECC
RLTTPLLRRQRGEAFQPVSWEAAIAFITAKLSAIKERHGPDAIMMTGSSRGPGNEANYVM
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
QKFARAAIGTNNIDCCARLCHGPSVAGLQQTLGNGAMSNSIGEIENTDCILVFGYNAADS
HHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHCCCCCCCCCCCEEEEEECCCCCC
HPIVARRILKAKNKGAKLIVCDPRHIETARIADQWLPLKNGANMALVNAFIHVLIEEKLY
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
DANYVRDHTRGFDELKAGVAAYRPEDVEPITGLSAKAVRQAMRTYAAAPAATLLWGMGVT
CCHHHHHHHCCHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHH
QWGQAVDVVKGLSSLALLTGNLGRPNVGVGPVRGQNNVQGACDMGVLPNTFPGYEKVTDS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCH
EARARFAHAWGVAELPAHVGYSITDVPHKVAEGKLKAYYVFGEDPIQTEPDLAAMRAAFA
HHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHH
ALDLVVVQDIFMTKTAELADVVLPATSWGEHEGVYTSADRGFQHFNKAIEPKGDVKPDWE
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCCCHH
IISLISTAMGYPMAYQNTQQIWDEMRGLCPPFAGATYDKLAGLGYVQWPCPTEDHPGTPY
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCE
LYTDNQFATADGKGLLSCAPWQPPHEQTDSDYPLALCTVREVGHYSCRSMTGNCATLKIL
EEECCEEEECCCCCCEEECCCCCCCCCCCCCCCEEEEEHHHHCCHHHHCCCCCCEEEEEE
ADEPGYVQIHPEDASYLGVIDQALIWISSRRGKVIARASVTERANRGAVYMTYQWWIGAC
ECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCEEEEEEEEECCCC
NELTVDHVDPVSKTPEFKHCAVKVEAISDQTAAEQYVEQQYSQLKKRLSDAAVVP
CCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKILTVCPYCGSGCKLNLLVEDGEIVGAEPADGVTNQGALCLKGYYGWDFLNDTRLLTP
CCCCEEECCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCEEECC
RLTTPLLRRQRGEAFQPVSWEAAIAFITAKLSAIKERHGPDAIMMTGSSRGPGNEANYVM
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
QKFARAAIGTNNIDCCARLCHGPSVAGLQQTLGNGAMSNSIGEIENTDCILVFGYNAADS
HHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCHHCCCCCCCCCCCEEEEEECCCCCC
HPIVARRILKAKNKGAKLIVCDPRHIETARIADQWLPLKNGANMALVNAFIHVLIEEKLY
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
DANYVRDHTRGFDELKAGVAAYRPEDVEPITGLSAKAVRQAMRTYAAAPAATLLWGMGVT
CCHHHHHHHCCHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHH
QWGQAVDVVKGLSSLALLTGNLGRPNVGVGPVRGQNNVQGACDMGVLPNTFPGYEKVTDS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCH
EARARFAHAWGVAELPAHVGYSITDVPHKVAEGKLKAYYVFGEDPIQTEPDLAAMRAAFA
HHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHH
ALDLVVVQDIFMTKTAELADVVLPATSWGEHEGVYTSADRGFQHFNKAIEPKGDVKPDWE
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCCCHH
IISLISTAMGYPMAYQNTQQIWDEMRGLCPPFAGATYDKLAGLGYVQWPCPTEDHPGTPY
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCE
LYTDNQFATADGKGLLSCAPWQPPHEQTDSDYPLALCTVREVGHYSCRSMTGNCATLKIL
EEECCEEEECCCCCCEEECCCCCCCCCCCCCCCEEEEEHHHHCCHHHHCCCCCCEEEEEE
ADEPGYVQIHPEDASYLGVIDQALIWISSRRGKVIARASVTERANRGAVYMTYQWWIGAC
ECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCEEEEEEEEECCCC
NELTVDHVDPVSKTPEFKHCAVKVEAISDQTAAEQYVEQQYSQLKKRLSDAAVVP
CCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2941757; 8265357; 9278503; 2211698; 9036855 [H]