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The map label for this gene is gyrB [H]

Identifier: 153947743

GI number: 153947743

Start: 4684094

End: 4686508

Strand: Reverse

Name: gyrB [H]

Synonym: YpsIP31758_4150

Alternate gene names: 153947743

Gene position: 4686508-4684094 (Counterclockwise)

Preceding gene: 153947001

Following gene: 153947940

Centisome position: 99.22

GC content: 49.57

Gene sequence:

>2415_bases
ATGTCGAATACTTATGACTCCTCAAGTATCAAGGTATTAAAAGGGCTGGATGCCGTGCGTAAGCGTCCGGGCATGTATAT
CGGAGATACTGATGATGGTACCGGTCTGCACCACATGGTATTCGAGGTTGTGGACAACGCTATTGACGAAGCCCTCGCGG
GTCATTGTAAAGAAATTCTGGTAACCATCCACGCGGATAACTCCGTTTCTGTACAGGATGATGGCCGTGGTATCCCAACC
GGTATGCACGATGAGGAAGGGGTATCTGCAGCAGAGGTCATCATGACCGTGTTGCATGCTGGCGGTAAATTTGACGATAA
CTCCTATAAAGTTTCCGGCGGTTTGCATGGCGTAGGGGTATCCGTTGTTAACGCCCTGTCTGAAAAGTTGGAACTGGTTA
TTCGCCGTGAAGGTAAAGTTCACGAGCAAACCTACAAAATGGGCGTGCCACAGGCACCGCTGAAAGTGGTGGGTGAAACT
GAGCAAACCGGGACCACTGTGCGTTTCTGGCCAAGTTTCCAGACCTTTACCAATAACACCGAATTCCAGTATGAGATCCT
GGCTAAACGCCTGCGCGAACTCTCTTTCCTGAACTCGGGTGTTTCAATCAAACTGAAAGACAAACGTAACGATAAAGAAG
ACCACTTCCATTACGAAGGTGGTATCAAGGCGTTTGTTGAATATCTGAACAAAAACAAGAACCCGATCCACCCGAAAGTG
TTCTACTTCTCCACCATGAAAGACGATATCGGGGTGGAAGTGGCGCTACAGTGGAACGATGGTTTCCAGGAAAATATTTA
CTGCTTCACCAATAACATTCCACAGCGTGATGGTGGTACCCATTTAGTTGGTTTCCGTACGGCGATGACCCGTACCCTGA
ACAGCTATATGGATAAAGAAGGCTACAGCAAAAAAGCGAAGATCAGCGCCACCGGTGATGACGCCCGTGAAGGCTTGATT
GCTGTTGTCTCAGTGAAAGTGCCCGATCCTAAATTCTCCTCACAGACCAAAGATAAATTGGTTTCTTCTGAGGTGAAAAC
GGCGGTCGAAACGCTGATGAACGAGAAGTTGGTTGAGTATTTGTTGGAAAACCCAACTGACGCCAAAATCGTTGTGGGTA
AAATCATTGATGCGGCCCGCGCCCGTGAAGCTGCGCGTAAAGCCCGTGAAATGACACGCCGTAAAGGCGCGCTCGATCTG
GCGGGTTTACCCGGCAAACTGGCAGACTGTCAGGAACGTGACCCGGCATTGTCCGAACTCTACTTAGTGGAAGGGGACTC
TGCGGGCGGCTCGGCTAAACAAGGCCGTAACCGTAAAAATCAGGCCATTTTGCCGCTGAAAGGTAAAATCCTGAACGTTG
AGAAAGCGCGTTTTGACAAAATGCTCTCTTCACAGGAAGTGGCGACACTGATCACCGCGTTAGGTTGTGGTATTGGCCGG
GATGAATATAACCCGGATAAATTGCGTTATCACAATATCATTATCATGACCGATGCCGACGTGGATGGCTCGCACATCCG
TACGTTGTTGTTGACCTTCTTCTACCGTCAGATGCCGGAAATTATTGAGCGTGGTCATGTGTTTATCGCTCAGCCTCCGC
TGTATAAAGTGAAGAAAGGTAAGCAAGAGCAGTACATCAAAGATGATGATGCGATGGAGCAGTACCAAATGACACTGGCT
CTGGATGGCGCGGCATTGCATACCAATGCTCACGCACCAGCACTGGCTGGTGAGCCTTTGGAGAAACTGGTTGCTGAGCA
TTACAGCGTCCAGAAGACTATCGCCCGCATGGAACGCCGTTATCCGCGTGCTTTGCTGAATAACCTGATTTATCAGCCTA
CCTTGCAAGAAGAGGATTTGGGCAATAAAGAGGTTGTGACGCAATGGATTGATTCGCTGGTGGTCTTGCTGAACGAAAAA
GAACACCATGGCAGCAGCTACAGTTCAGTGGTGCGTGAGAACCGTGAGCGTCAACTGTTCGAGCCTATTTTGCGTATTCG
TACCCACGGGGTTGATACCGATTATGATCTGGACTTCGATTTTATCCATGGTGGCGAGTACCGTAAAATCTGTCTATTGG
GTGACAAACTGCGTGGCTTAATCGAAGACGGTGCTTTCGTTGAACGTGGCGAGCGTCGTGTGCCGGTTTCAAGCTTCGAA
GAGGCGCTGGACTGGTTAGTGAAAGAGTCCCGCCGTGGTTTGGCAATCCAGCGTTACAAAGGCTTGGGTGAAATGAACCC
CGATCAGTTGTGGGAAACCACGATGGATCCAGAGAGCCGTCGTATGCTGCGAGTCATGGTGAAAGATGCCATCGCCGCCG
ATCAACTCTTCACTACTCTGATGGGTGATGCTGTTGAACCTCGTCGTGCCTTTATCGAAGAGAATGCACTGCGCGCAGCC
AATATTGATATCTAA

Upstream 100 bases:

>100_bases
CAAGTTGCCGATATGGTAGGTGAAAAGGGCAAGATGTTCCGTGTGGAACATGGCAAAATAGAGGTTCAACCACAGGATTA
AAAATGAGCGAGAAACGTTG

Downstream 100 bases:

>100_bases
TGTGTTAGCCAGATAGCCTATTGGCCAGATAAAAAAGTCCAGGGTGGGAAAACTGCCACTGGGCTTTTTTTTGCCTGAAA
ATTGTTATTGTTACCTGATG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MSNTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEILVTIHADNSVSVQDDGRGIPT
GMHDEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKLELVIRREGKVHEQTYKMGVPQAPLKVVGET
EQTGTTVRFWPSFQTFTNNTEFQYEILAKRLRELSFLNSGVSIKLKDKRNDKEDHFHYEGGIKAFVEYLNKNKNPIHPKV
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKEGYSKKAKISATGDDAREGLI
AVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVEYLLENPTDAKIVVGKIIDAARAREAARKAREMTRRKGALDL
AGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKGKQEQYIKDDDAMEQYQMTLA
LDGAALHTNAHAPALAGEPLEKLVAEHYSVQKTIARMERRYPRALLNNLIYQPTLQEEDLGNKEVVTQWIDSLVVLLNEK
EHHGSSYSSVVRENRERQLFEPILRIRTHGVDTDYDLDFDFIHGGEYRKICLLGDKLRGLIEDGAFVERGERRVPVSSFE
EALDWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVMVKDAIAADQLFTTLMGDAVEPRRAFIEENALRAA
NIDI

Sequences:

>Translated_804_residues
MSNTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEILVTIHADNSVSVQDDGRGIPT
GMHDEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKLELVIRREGKVHEQTYKMGVPQAPLKVVGET
EQTGTTVRFWPSFQTFTNNTEFQYEILAKRLRELSFLNSGVSIKLKDKRNDKEDHFHYEGGIKAFVEYLNKNKNPIHPKV
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKEGYSKKAKISATGDDAREGLI
AVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVEYLLENPTDAKIVVGKIIDAARAREAARKAREMTRRKGALDL
AGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKGKQEQYIKDDDAMEQYQMTLA
LDGAALHTNAHAPALAGEPLEKLVAEHYSVQKTIARMERRYPRALLNNLIYQPTLQEEDLGNKEVVTQWIDSLVVLLNEK
EHHGSSYSSVVRENRERQLFEPILRIRTHGVDTDYDLDFDFIHGGEYRKICLLGDKLRGLIEDGAFVERGERRVPVSSFE
EALDWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVMVKDAIAADQLFTTLMGDAVEPRRAFIEENALRAA
NIDI
>Mature_803_residues
SNTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEILVTIHADNSVSVQDDGRGIPTG
MHDEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKLELVIRREGKVHEQTYKMGVPQAPLKVVGETE
QTGTTVRFWPSFQTFTNNTEFQYEILAKRLRELSFLNSGVSIKLKDKRNDKEDHFHYEGGIKAFVEYLNKNKNPIHPKVF
YFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKEGYSKKAKISATGDDAREGLIA
VVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVEYLLENPTDAKIVVGKIIDAARAREAARKAREMTRRKGALDLA
GLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRD
EYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKGKQEQYIKDDDAMEQYQMTLAL
DGAALHTNAHAPALAGEPLEKLVAEHYSVQKTIARMERRYPRALLNNLIYQPTLQEEDLGNKEVVTQWIDSLVVLLNEKE
HHGSSYSSVVRENRERQLFEPILRIRTHGVDTDYDLDFDFIHGGEYRKICLLGDKLRGLIEDGAFVERGERRVPVSSFEE
ALDWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVMVKDAIAADQLFTTLMGDAVEPRRAFIEENALRAAN
IDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=574, Percent_Identity=26.1324041811847, Blast_Score=131, Evalue=3e-30,
Organism=Homo sapiens, GI19913408, Length=551, Percent_Identity=25.2268602540835, Blast_Score=122, Evalue=2e-27,
Organism=Escherichia coli, GI48994957, Length=804, Percent_Identity=86.9402985074627, Blast_Score=1442, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=552, Percent_Identity=41.4855072463768, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535065, Length=539, Percent_Identity=27.4582560296846, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=519, Percent_Identity=24.0847784200385, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=589, Percent_Identity=24.4482173174873, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI17136538, Length=542, Percent_Identity=24.9077490774908, Blast_Score=134, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90269; Mature: 90138

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIL
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
VTIHADNSVSVQDDGRGIPTGMHDEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKLELVIRREGKVHEQTYKMGVPQAPLKVVGETEQTGTTVRFWPSFQTFTNNT
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCHHHHCCCC
EFQYEILAKRLRELSFLNSGVSIKLKDKRNDKEDHFHYEGGIKAFVEYLNKNKNPIHPKV
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCEE
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKE
EEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GYSKKAKISATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVEY
CCCCCEEEEECCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLENPTDAKIVVGKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQERDPALSEL
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCC
KQEQYIKDDDAMEQYQMTLALDGAALHTNAHAPALAGEPLEKLVAEHYSVQKTIARMERR
CCHHCCCCCHHHHHHEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YPRALLNNLIYQPTLQEEDLGNKEVVTQWIDSLVVLLNEKEHHGSSYSSVVRENRERQLF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
EPILRIRTHGVDTDYDLDFDFIHGGEYRKICLLGDKLRGLIEDGAFVERGERRVPVSSFE
HHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECHHHHHHHHCCHHHHCCCCCCCHHHHH
EALDWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVMVKDAIAADQLFTTL
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGDAVEPRRAFIEENALRAANIDI
HCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIL
CCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEE
VTIHADNSVSVQDDGRGIPTGMHDEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKLELVIRREGKVHEQTYKMGVPQAPLKVVGETEQTGTTVRFWPSFQTFTNNT
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCHHHHCCCC
EFQYEILAKRLRELSFLNSGVSIKLKDKRNDKEDHFHYEGGIKAFVEYLNKNKNPIHPKV
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCEE
FYFSTMKDDIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLVGFRTAMTRTLNSYMDKE
EEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
GYSKKAKISATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKTAVETLMNEKLVEY
CCCCCEEEEECCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLENPTDAKIVVGKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQERDPALSEL
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIIERGHVFIAQPPLYKVKKG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCC
KQEQYIKDDDAMEQYQMTLALDGAALHTNAHAPALAGEPLEKLVAEHYSVQKTIARMERR
CCHHCCCCCHHHHHHEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YPRALLNNLIYQPTLQEEDLGNKEVVTQWIDSLVVLLNEKEHHGSSYSSVVRENRERQLF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
EPILRIRTHGVDTDYDLDFDFIHGGEYRKICLLGDKLRGLIEDGAFVERGERRVPVSSFE
HHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECHHHHHHHHCCHHHHCCCCCCCHHHHH
EALDWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVMVKDAIAADQLFTTL
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGDAVEPRRAFIEENALRAANIDI
HCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]