Click here to switch to the map view.

The map label for this gene is recF [H]

Identifier: 153947001

GI number: 153947001

Start: 4686528

End: 4687613

Strand: Reverse

Name: recF [H]

Synonym: YpsIP31758_4151

Alternate gene names: 153947001

Gene position: 4687613-4686528 (Counterclockwise)

Preceding gene: 153950603

Following gene: 153947743

Centisome position: 99.24

GC content: 52.76

Gene sequence:

>1086_bases
ATGGCTCTAACGCGTTTACTCATTAAAGATTTTCGCAATATCGAATCGGCAGACTTGGCGCTGGCGGCGGGCTTTAATTT
TTTAGTCGGCCCCAATGGCAGTGGCAAAACCAGTGTACTCGAGGCGGTTTATACCCTTGGTCATGGTAGGGCTTTTCGTA
GTTTGCAAGCAGGCAGAGTGATACGTCACGAATGTGCTGAGTTTGTGTTGCATGGCCGGGTTGACGCCAATGAACGTGAG
GCGTCAGTGGGTCTGAGCAAAAGCCGACAGGGTGATACCAAAGTGCGCATTGATGGCACCGATGGGCATAAAGTGGCTGA
ATTGGCGCAAATGTTGCCAATGCAGTTAATCACTCCTGAAGGTTTTACGTTATTAAATGGCGGGCCAAAATTCCGGCGCG
CTTTTTTAGACTGGGGTTGCTTTCATAATGAACCCGGTTTTTTTACGGCCTGGAGCAACCTAAAAAGGTTACTCAAGCAG
CGCAACGCCGCGCTGCGCCAGGTTAGTCGCTATACGCAAATCCGGGCTTGGGATCAGGAAATCATCCCTCTGGCTGAGCG
TATCAGCGAATGGCGAGCGGCATACAGTGACGCGATTGCGGCGGATATTTCAGCCACATGCGCTCTGTTCCTGCCAGAGT
TCGCCCTGAGTTTCTCTTTCCAGCGCGGCTGGGATAAAGAGAGTGACTATGGCGAGTTGTTGGAGCGCCAGTTTGAGCGT
GACAGAGCGCTAACGTATACCGCCGTTGGGCCACATAAAGCGGATTTCCGCATTCGGGCCGATGGTACGCCGGTAGAAGA
TTTGCTATCGCGTGGTCAGCTTAAGTTGCTGATGTGCGCACTGCGGTTAGCGCAGGGTGAGTTTCTCACCCGACAGAGCG
GGCGGCGTTGCCTGTATCTGCTTGACGATTTTGCTTCCGAATTGGACACCGGCCGCCGTCGATTGCTGGCCGAGCGTTTG
AAAGCTACCCAGGCACAAGTTTTTGTCAGCGCCGTGAGCGCTGAGCAAGTTGCCGATATGGTAGGTGAAAAGGGCAAGAT
GTTCCGTGTGGAACATGGCAAAATAGAGGTTCAACCACAGGATTAA

Upstream 100 bases:

>100_bases
CTCCGGTGGCTGCGCGCTGGCCGCACGCAAATGGCCTGCGACGATAACGCCCTTGATCAGAAATAAGGCGACATTGCCTT
CGATCATAAGAATTAGTTTC

Downstream 100 bases:

>100_bases
AAATGAGCGAGAAACGTTGATGTCGAATACTTATGACTCCTCAAGTATCAAGGTATTAAAAGGGCTGGATGCCGTGCGTA
AGCGTCCGGGCATGTATATC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MALTRLLIKDFRNIESADLALAAGFNFLVGPNGSGKTSVLEAVYTLGHGRAFRSLQAGRVIRHECAEFVLHGRVDANERE
ASVGLSKSRQGDTKVRIDGTDGHKVAELAQMLPMQLITPEGFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
RNAALRQVSRYTQIRAWDQEIIPLAERISEWRAAYSDAIAADISATCALFLPEFALSFSFQRGWDKESDYGELLERQFER
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYLLDDFASELDTGRRRLLAERL
KATQAQVFVSAVSAEQVADMVGEKGKMFRVEHGKIEVQPQD

Sequences:

>Translated_361_residues
MALTRLLIKDFRNIESADLALAAGFNFLVGPNGSGKTSVLEAVYTLGHGRAFRSLQAGRVIRHECAEFVLHGRVDANERE
ASVGLSKSRQGDTKVRIDGTDGHKVAELAQMLPMQLITPEGFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
RNAALRQVSRYTQIRAWDQEIIPLAERISEWRAAYSDAIAADISATCALFLPEFALSFSFQRGWDKESDYGELLERQFER
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYLLDDFASELDTGRRRLLAERL
KATQAQVFVSAVSAEQVADMVGEKGKMFRVEHGKIEVQPQD
>Mature_360_residues
ALTRLLIKDFRNIESADLALAAGFNFLVGPNGSGKTSVLEAVYTLGHGRAFRSLQAGRVIRHECAEFVLHGRVDANEREA
SVGLSKSRQGDTKVRIDGTDGHKVAELAQMLPMQLITPEGFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQR
NAALRQVSRYTQIRAWDQEIIPLAERISEWRAAYSDAIAADISATCALFLPEFALSFSFQRGWDKESDYGELLERQFERD
RALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYLLDDFASELDTGRRRLLAERLK
ATQAQVFVSAVSAEQVADMVGEKGKMFRVEHGKIEVQPQD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=355, Percent_Identity=80, Blast_Score=600, Evalue=1e-173,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40527; Mature: 40396

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTRLLIKDFRNIESADLALAAGFNFLVGPNGSGKTSVLEAVYTLGHGRAFRSLQAGRV
CHHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
IRHECAEFVLHGRVDANEREASVGLSKSRQGDTKVRIDGTDGHKVAELAQMLPMQLITPE
HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHEECCC
GFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVSRYTQIRAWDQE
CCEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIPLAERISEWRAAYSDAIAADISATCALFLPEFALSFSFQRGWDKESDYGELLERQFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYL
CCCEEEEECCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCEEEEH
LDDFASELDTGRRRLLAERLKATQAQVFVSAVSAEQVADMVGEKGKMFRVEHGKIEVQPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECC
D
C
>Mature Secondary Structure 
ALTRLLIKDFRNIESADLALAAGFNFLVGPNGSGKTSVLEAVYTLGHGRAFRSLQAGRV
HHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
IRHECAEFVLHGRVDANEREASVGLSKSRQGDTKVRIDGTDGHKVAELAQMLPMQLITPE
HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCHHEECCC
GFTLLNGGPKFRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVSRYTQIRAWDQE
CCEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIPLAERISEWRAAYSDAIAADISATCALFLPEFALSFSFQRGWDKESDYGELLERQFER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
DRALTYTAVGPHKADFRIRADGTPVEDLLSRGQLKLLMCALRLAQGEFLTRQSGRRCLYL
CCCEEEEECCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCEEEEH
LDDFASELDTGRRRLLAERLKATQAQVFVSAVSAEQVADMVGEKGKMFRVEHGKIEVQPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA