Click here to switch to the map view.

The map label for this gene is argF

Identifier: 153947545

GI number: 153947545

Start: 4009885

End: 4010892

Strand: Direct

Name: argF

Synonym: YpsIP31758_3549

Alternate gene names: 153947545

Gene position: 4009885-4010892 (Clockwise)

Preceding gene: 153950574

Following gene: 153949524

Centisome position: 84.9

GC content: 49.6

Gene sequence:

>1008_bases
ATGAATCAGTTCTATAAACGTCACTTTCTGAGGTTGTTAGATTTTACCCCCGCTGAAATTATTGCCTTGCTTGATTTGGC
TACTGAGTTGAAAAAAGACAAGAAATCCGGCTGTGAACAGCAAAAATTGGTCGGTAAGAATATCGCGCTCATCTTCGAAA
AAGACTCGACTCGCACTCGATGCTCTTTCGAAGTTGCCGCATATGACCAAGGTGCTCGCGTCACTTATCTCGGGCCAGGG
GGAAGCCAGATTGGGCATAAAGAATCAATCAAGGATACCGCCCGCGTATTAGGCCGTATGTATGACGGCATTCAATACCG
TGGCTATGGGCAACGCGTGGTGGAAACACTCGCAGAATTTGCCGGCGTACCGGTCTGGAATGGCCTGACCAATGAGTTTC
ATCCAACCCAACTCCTGGCTGATTTGTTGACGATGCGGGAACATCTGCCCAATAAATCGCTGAATAAAATGACGTTGGCG
TACCTCGGTGATACACGTAATAACATGGGGAACAGCCTGTTAGAAGCGGCCGCACTGGTAGGAATGGATTTACGCCTGGT
CGCACCGAAAGCCTGTTGGCCAGAAGAAGCCTTCGTTATCAGTTGTCAGGCACTGGCACAGAAAACGGGGGGCAAAATCA
CCCTGACAGAAGATATTGCCGAGGGAGTGAACGGCGCTGATTTTCTTTATACCGATGTCTGGGTCTCGATGGGGGAGCCC
AAAGAGGTCTGGCAGGAACGCATCACCTTGCTCAAACCTTATCAGGTCAATATGCGTGTACTGGCGCTGACCGGTAACCC
TCAGGTTAAATTTCTGCACTGCCTCCCCGCCTTCCACGATGACCAAACCACCATCGGTAAGCAGATGGCCGAACAATATG
ATTTACCCGGCGGCATGGAAGTGACTGAAGAGGTGTTTGAATCAGCTCATAGCATTGTATTTGATCAGGCAGAAAACCGC
CTGCATACCATCAAAGCCGTGATGGTCGCCACGATGAGCAAAATATAA

Upstream 100 bases:

>100_bases
TATACCTATAAATTGAATTTAAATTCACATTGAAATTCAATTTACCCCCAAATAATTGGTGTTGTAGCATCAAGGATGAA
GGGCACATGGAGACGAACAG

Downstream 100 bases:

>100_bases
AGGCAGCCGATGACAATAATAAGGGCTGATCCGTTATCTGTATCGGCCCTTTACCTATAAAATTCAGACGGCAGCGAGCA
CTGCCCCGAAGCTCAAACTG

Product: ornithine carbamoyltransferase subunit I

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNQFYKRHFLRLLDFTPAEIIALLDLATELKKDKKSGCEQQKLVGKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPG
GSQIGHKESIKDTARVLGRMYDGIQYRGYGQRVVETLAEFAGVPVWNGLTNEFHPTQLLADLLTMREHLPNKSLNKMTLA
YLGDTRNNMGNSLLEAAALVGMDLRLVAPKACWPEEAFVISCQALAQKTGGKITLTEDIAEGVNGADFLYTDVWVSMGEP
KEVWQERITLLKPYQVNMRVLALTGNPQVKFLHCLPAFHDDQTTIGKQMAEQYDLPGGMEVTEEVFESAHSIVFDQAENR
LHTIKAVMVATMSKI

Sequences:

>Translated_335_residues
MNQFYKRHFLRLLDFTPAEIIALLDLATELKKDKKSGCEQQKLVGKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPG
GSQIGHKESIKDTARVLGRMYDGIQYRGYGQRVVETLAEFAGVPVWNGLTNEFHPTQLLADLLTMREHLPNKSLNKMTLA
YLGDTRNNMGNSLLEAAALVGMDLRLVAPKACWPEEAFVISCQALAQKTGGKITLTEDIAEGVNGADFLYTDVWVSMGEP
KEVWQERITLLKPYQVNMRVLALTGNPQVKFLHCLPAFHDDQTTIGKQMAEQYDLPGGMEVTEEVFESAHSIVFDQAENR
LHTIKAVMVATMSKI
>Mature_335_residues
MNQFYKRHFLRLLDFTPAEIIALLDLATELKKDKKSGCEQQKLVGKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPG
GSQIGHKESIKDTARVLGRMYDGIQYRGYGQRVVETLAEFAGVPVWNGLTNEFHPTQLLADLLTMREHLPNKSLNKMTLA
YLGDTRNNMGNSLLEAAALVGMDLRLVAPKACWPEEAFVISCQALAQKTGGKITLTEDIAEGVNGADFLYTDVWVSMGEP
KEVWQERITLLKPYQVNMRVLALTGNPQVKFLHCLPAFHDDQTTIGKQMAEQYDLPGGMEVTEEVFESAHSIVFDQAENR
LHTIKAVMVATMSKI

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=332, Percent_Identity=37.6506024096386, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI18105007, Length=336, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1790703, Length=334, Percent_Identity=79.940119760479, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=78.7425149700599, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI48994908, Length=375, Percent_Identity=24.2666666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367364, Length=340, Percent_Identity=25.8823529411765, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=224, Percent_Identity=31.25, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322373, Length=329, Percent_Identity=32.8267477203647, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6322331, Length=167, Percent_Identity=31.1377245508982, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24642586, Length=326, Percent_Identity=26.0736196319018, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_YERP3 (A7FMM5)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402503.1
- ProteinModelPortal:   A7FMM5
- SMR:   A7FMM5
- STRING:   A7FMM5
- GeneID:   5386193
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3549
- NMPDR:   fig|349747.3.peg.3166
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   DFRIFAP
- ProtClustDB:   PRK03515
- BioCyc:   YPSE349747:YPSIP31758_3549-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 37435; Mature: 37435

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 107-107 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFYKRHFLRLLDFTPAEIIALLDLATELKKDKKSGCEQQKLVGKNIALIFEKDSTRTR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCE
CSFEVAAYDQGARVTYLGPGGSQIGHKESIKDTARVLGRMYDGIQYRGYGQRVVETLAEF
EEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHH
AGVPVWNGLTNEFHPTQLLADLLTMREHLPNKSLNKMTLAYLGDTRNNMGNSLLEAAALV
HCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
GMDLRLVAPKACWPEEAFVISCQALAQKTGGKITLTEDIAEGVNGADFLYTDVWVSMGEP
CCCEEEECCCCCCCCCHHEEEHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHCCCCH
KEVWQERITLLKPYQVNMRVLALTGNPQVKFLHCLPAFHDDQTTIGKQMAEQYDLPGGME
HHHHHHHHHEECCEEECEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCH
VTEEVFESAHSIVFDQAENRLHTIKAVMVATMSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQFYKRHFLRLLDFTPAEIIALLDLATELKKDKKSGCEQQKLVGKNIALIFEKDSTRTR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCE
CSFEVAAYDQGARVTYLGPGGSQIGHKESIKDTARVLGRMYDGIQYRGYGQRVVETLAEF
EEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHH
AGVPVWNGLTNEFHPTQLLADLLTMREHLPNKSLNKMTLAYLGDTRNNMGNSLLEAAALV
HCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
GMDLRLVAPKACWPEEAFVISCQALAQKTGGKITLTEDIAEGVNGADFLYTDVWVSMGEP
CCCEEEECCCCCCCCCHHEEEHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHCCCCH
KEVWQERITLLKPYQVNMRVLALTGNPQVKFLHCLPAFHDDQTTIGKQMAEQYDLPGGME
HHHHHHHHHEECCEEECEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCH
VTEEVFESAHSIVFDQAENRLHTIKAVMVATMSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA