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The map label for this gene is dgkA [H]

Identifier: 153947443

GI number: 153947443

Start: 4249408

End: 4249779

Strand: Reverse

Name: dgkA [H]

Synonym: YpsIP31758_3771

Alternate gene names: 153947443

Gene position: 4249779-4249408 (Counterclockwise)

Preceding gene: 153947917

Following gene: 153950737

Centisome position: 89.97

GC content: 47.85

Gene sequence:

>372_bases
ATGGCGAATCAATCAACAGGACTTACCCGGATCTATAAAGCCGCGGGTTATACGGTGAAAGGGTTAACCGCCGCCTGGAA
TAATGAAGCGGCTTTCCGTCAAGAAAGCGTTGCTGCCGTCATCGCTATCATACTGGCCTTCTGGTTGGATGTGGGGGCCA
TAGCGCGCATTTTATTAATTTGCAGCGTTGTTTTGGTGCTCATTGTTGAAGTCATTAACAGTGCGATTGAAGCGGTAGTT
GATAGGATCGGTAGTGAATTTCATGCGCTTTCTGGACGGGCAAAAGATATGGGGTCTGCTGCTGTGTTTTTGACCATTTT
GATGGCGCTATTCGTATGGATAACAGTACTCTGGCAGCATGTCGCTCGCTAA

Upstream 100 bases:

>100_bases
TCGTCGCAAGGATACCAGAAACTGGTTTTGAGATCTGTGGAGATGAGACAACAGAAAATGGCAAAAATCACGATTAACAT
AAGATAAAGGCAAATAAACC

Downstream 100 bases:

>100_bases
TGAGGCTAAGGGTGCTGTTAAAATGAGCAGTTATTAATCCCTGTTTTTATTCACTCTTTGGTTCCCAACACCCGCTTAGC
TGTATATACTCACAGCAAAA

Product: diacylglycerol kinase

Products: NA

Alternate protein names: DAGK; Diglyceride kinase; DGK [H]

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MANQSTGLTRIYKAAGYTVKGLTAAWNNEAAFRQESVAAVIAIILAFWLDVGAIARILLICSVVLVLIVEVINSAIEAVV
DRIGSEFHALSGRAKDMGSAAVFLTILMALFVWITVLWQHVAR

Sequences:

>Translated_123_residues
MANQSTGLTRIYKAAGYTVKGLTAAWNNEAAFRQESVAAVIAIILAFWLDVGAIARILLICSVVLVLIVEVINSAIEAVV
DRIGSEFHALSGRAKDMGSAAVFLTILMALFVWITVLWQHVAR
>Mature_122_residues
ANQSTGLTRIYKAAGYTVKGLTAAWNNEAAFRQESVAAVIAIILAFWLDVGAIARILLICSVVLVLIVEVINSAIEAVVD
RIGSEFHALSGRAKDMGSAAVFLTILMALFVWITVLWQHVAR

Specific function: Recycling of diacylglycerol produced during the turnover of membrane phospholipid [H]

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family [H]

Homologues:

Organism=Escherichia coli, GI1790475, Length=120, Percent_Identity=68.3333333333333, Blast_Score=167, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000829 [H]

Pfam domain/function: PF01219 DAGK_prokar [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 13287; Mature: 13156

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS01069 DAGK_PROKAR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQSTGLTRIYKAAGYTVKGLTAAWNNEAAFRQESVAAVIAIILAFWLDVGAIARILLI
CCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSVVLVLIVEVINSAIEAVVDRIGSEFHALSGRAKDMGSAAVFLTILMALFVWITVLWQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAR
HCC
>Mature Secondary Structure 
ANQSTGLTRIYKAAGYTVKGLTAAWNNEAAFRQESVAAVIAIILAFWLDVGAIARILLI
CCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CSVVLVLIVEVINSAIEAVVDRIGSEFHALSGRAKDMGSAAVFLTILMALFVWITVLWQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]