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The map label for this gene is bfpB [H]

Identifier: 153947286

GI number: 153947286

Start: 4209185

End: 4210777

Strand: Reverse

Name: bfpB [H]

Synonym: YpsIP31758_3734

Alternate gene names: 153947286

Gene position: 4210777-4209185 (Counterclockwise)

Preceding gene: 153948904

Following gene: 153948492

Centisome position: 89.15

GC content: 50.47

Gene sequence:

>1593_bases
ATGCATTGGTATTCCGTAGCCCGAACCTCGCGCCTCATTGGTGTGGTACTCAGTCTAAAATTTTTGATTGGCTGTTCACT
TAGTGAAATTGACCGGATAGAAAGCCTGGCAAAAGATAGCGGCGAAACCGCTCGGCGTATTACGGATAGCAATGGCGCTA
AAACTCAGCCTTCAGTGACTTGGGCCGATAAGCCGTGGGTGAATTTGAAACCGCTCACACCTGCTGCCGCAAGCCCTGAA
GAGAAAATGCTGCTTCCTTGCCAGATAACTATCAACCGCCCTGAAGGCATGAGTCTACCTGAACTGGGGCAGCGTATTAC
CCGCTTGTGTGGTGCCCGCGTGACCATCACACCAGATGCCTTTTTGGCTTTATCGTCTGCTAACGCCAGTTTCGACATGA
TGCCGTCCGGCAAGCATATTAATGCACTGAAATGGCAGGGTGATTTGCCGGGTTTGTTGGACACCATCGGCAGTCGTTTG
GGGCTATCATGGCGTACTGACAAAGGTAATATTGTCTTTTATCAATTGGATACTCGAACTTTCCAACTCGTCATCCTTAA
TACCAAAATTAGCAGCTCCGCCAGTGTAACGTCAGGATCCTCCAGCTCGATGGGCTCAAGCGGCGGAGCCAATAGTGGTG
TGTCTGGCGATTCGAGTTCCGTACAGAAAACCTCGGTTGATCTGAACAGTAATCTCTACGAGGACATTAAAAAGACGATT
GAGAATATGCTGACACCGTCTAAAGGGCGCTTTTGGCTCTCTTCTGCCAGTGGTACCTTGACGGTGACGGACACGCCTGA
AACCCTAGAGCGGATAGGGCGTTATATCGACCATCAGAATGAGCTTCTCAACCGCCAGGTTCAGCTTAATGTTCAGGTTT
TGAGTGTTACCCAGTCGCGCAAGGAGCAGTTTGGTCTGGACTGGAAACTGGTGTATCAATCGTTAAACAATATCGGTGCC
AGCGTGACGGGAAACCTGATTAACGTGGCAAATAATACCCTCTCCGGTGGTCTGTCTATTCTGGACACTGCGACGGGGCC
CGCAGAGAAGTTTTCTGGATCGGAGCTGCTGATCAAAGCGCTTTCTCAGCAGGGTAACGTCAGTTTGGTCACGGAACTCA
ACAGGGCGACAATCAACCTGACACCGGTACCGTTCCAGATCTCCGATCAAACGGGATACATACAAAGCTCAAGCACGACG
GTAACCGCCAACGCGGGTACTACATCGTCAATGCAGACCGGCATTATAACCACGGGCCTGTTTATGTCGATGTTGCCTTA
CATTCAGGAAAATGGCGATGTACAGCTCCAGTTTGCTTTCACTCTTTCGGACAAACCCATCATTCAGCCTTTCTTTTCTC
GTGATGGTAATACGCGCAATGACACCCCTGCGTTCAAACTTCGTTCACTTACCCAAACAGTCAATCTGCGCGCGGGCCAG
ACATTGGTGCTGACCGGGGCCGATCAACTTGACACCGTAGCCAATAAACAAGGGGCATTTACCCCCAGTAACTTCTGGTG
GGGCGGCGGTCATAACGGTCTGGAAACCCGTACCACGCTGGTTATTCTTATTACACCGGTTCTGCTGAGGTAA

Upstream 100 bases:

>100_bases
GGGCGGCTGCGAATGGTTGATGGAACTGACATGAATCTCTCTCTTCCCGCCGGAGTGGCTGAAGGGAGTATTGTTTATCT
TAATTAAGGGAAAAAGCTGG

Downstream 100 bases:

>100_bases
TCATGAACAAGAATATTGCTGCTCGCCCTATCATGCCAGCCCAACAGGTAATGAGAATGGGGCGCTTGGGATGGGTGGCG
GGTATGAACTGGCAGATGCA

Product: type IVB pilus formation outer membrane protein

Products: NA

Alternate protein names: Bundle-forming pilus B [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MHWYSVARTSRLIGVVLSLKFLIGCSLSEIDRIESLAKDSGETARRITDSNGAKTQPSVTWADKPWVNLKPLTPAAASPE
EKMLLPCQITINRPEGMSLPELGQRITRLCGARVTITPDAFLALSSANASFDMMPSGKHINALKWQGDLPGLLDTIGSRL
GLSWRTDKGNIVFYQLDTRTFQLVILNTKISSSASVTSGSSSSMGSSGGANSGVSGDSSSVQKTSVDLNSNLYEDIKKTI
ENMLTPSKGRFWLSSASGTLTVTDTPETLERIGRYIDHQNELLNRQVQLNVQVLSVTQSRKEQFGLDWKLVYQSLNNIGA
SVTGNLINVANNTLSGGLSILDTATGPAEKFSGSELLIKALSQQGNVSLVTELNRATINLTPVPFQISDQTGYIQSSSTT
VTANAGTTSSMQTGIITTGLFMSMLPYIQENGDVQLQFAFTLSDKPIIQPFFSRDGNTRNDTPAFKLRSLTQTVNLRAGQ
TLVLTGADQLDTVANKQGAFTPSNFWWGGGHNGLETRTTLVILITPVLLR

Sequences:

>Translated_530_residues
MHWYSVARTSRLIGVVLSLKFLIGCSLSEIDRIESLAKDSGETARRITDSNGAKTQPSVTWADKPWVNLKPLTPAAASPE
EKMLLPCQITINRPEGMSLPELGQRITRLCGARVTITPDAFLALSSANASFDMMPSGKHINALKWQGDLPGLLDTIGSRL
GLSWRTDKGNIVFYQLDTRTFQLVILNTKISSSASVTSGSSSSMGSSGGANSGVSGDSSSVQKTSVDLNSNLYEDIKKTI
ENMLTPSKGRFWLSSASGTLTVTDTPETLERIGRYIDHQNELLNRQVQLNVQVLSVTQSRKEQFGLDWKLVYQSLNNIGA
SVTGNLINVANNTLSGGLSILDTATGPAEKFSGSELLIKALSQQGNVSLVTELNRATINLTPVPFQISDQTGYIQSSSTT
VTANAGTTSSMQTGIITTGLFMSMLPYIQENGDVQLQFAFTLSDKPIIQPFFSRDGNTRNDTPAFKLRSLTQTVNLRAGQ
TLVLTGADQLDTVANKQGAFTPSNFWWGGGHNGLETRTTLVILITPVLLR
>Mature_530_residues
MHWYSVARTSRLIGVVLSLKFLIGCSLSEIDRIESLAKDSGETARRITDSNGAKTQPSVTWADKPWVNLKPLTPAAASPE
EKMLLPCQITINRPEGMSLPELGQRITRLCGARVTITPDAFLALSSANASFDMMPSGKHINALKWQGDLPGLLDTIGSRL
GLSWRTDKGNIVFYQLDTRTFQLVILNTKISSSASVTSGSSSSMGSSGGANSGVSGDSSSVQKTSVDLNSNLYEDIKKTI
ENMLTPSKGRFWLSSASGTLTVTDTPETLERIGRYIDHQNELLNRQVQLNVQVLSVTQSRKEQFGLDWKLVYQSLNNIGA
SVTGNLINVANNTLSGGLSILDTATGPAEKFSGSELLIKALSQQGNVSLVTELNRATINLTPVPFQISDQTGYIQSSSTT
VTANAGTTSSMQTGIITTGLFMSMLPYIQENGDVQLQFAFTLSDKPIIQPFFSRDGNTRNDTPAFKLRSLTQTVNLRAGQ
TLVLTGADQLDTVANKQGAFTPSNFWWGGGHNGLETRTTLVILITPVLLR

Specific function: Is absolutely required for pilus biogenesis, and for EPEC localized adherence and autoaggregation. Acts at a step in the BFP biogenic pathway after production and processing of the structural pilus subunit BfpA [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011514
- InterPro:   IPR004846 [H]

Pfam domain/function: PF00263 Secretin; PF07655 Secretin_N_2 [H]

EC number: NA

Molecular weight: Translated: 57249; Mature: 57249

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHWYSVARTSRLIGVVLSLKFLIGCSLSEIDRIESLAKDSGETARRITDSNGAKTQPSVT
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCEE
WADKPWVNLKPLTPAAASPEEKMLLPCQITINRPEGMSLPELGQRITRLCGARVTITPDA
ECCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCC
FLALSSANASFDMMPSGKHINALKWQGDLPGLLDTIGSRLGLSWRTDKGNIVFYQLDTRT
EEEEECCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCEEECCCCCEEEEEECCCE
FQLVILNTKISSSASVTSGSSSSMGSSGGANSGVSGDSSSVQKTSVDLNSNLYEDIKKTI
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
ENMLTPSKGRFWLSSASGTLTVTDTPETLERIGRYIDHQNELLNRQVQLNVQVLSVTQSR
HHHCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCHHHHHHCCEEEEEEEEEEEHHHH
KEQFGLDWKLVYQSLNNIGASVTGNLINVANNTLSGGLSILDTATGPAEKFSGSELLIKA
HHHHCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
LSQQGNVSLVTELNRATINLTPVPFQISDQTGYIQSSSTTVTANAGTTSSMQTGIITTGL
HHCCCCEEEEEECCCEEEEECCCCEEECCCCCEEECCCEEEEECCCCCCCCCCCHHHHHH
FMSMLPYIQENGDVQLQFAFTLSDKPIIQPFFSRDGNTRNDTPAFKLRSLTQTVNLRAGQ
HHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
TLVLTGADQLDTVANKQGAFTPSNFWWGGGHNGLETRTTLVILITPVLLR
EEEEECCHHHHHHHCCCCCCCCCCCEECCCCCCCCHHEEEEEEECHHHCC
>Mature Secondary Structure
MHWYSVARTSRLIGVVLSLKFLIGCSLSEIDRIESLAKDSGETARRITDSNGAKTQPSVT
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCEE
WADKPWVNLKPLTPAAASPEEKMLLPCQITINRPEGMSLPELGQRITRLCGARVTITPDA
ECCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCC
FLALSSANASFDMMPSGKHINALKWQGDLPGLLDTIGSRLGLSWRTDKGNIVFYQLDTRT
EEEEECCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCEEECCCCCEEEEEECCCE
FQLVILNTKISSSASVTSGSSSSMGSSGGANSGVSGDSSSVQKTSVDLNSNLYEDIKKTI
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
ENMLTPSKGRFWLSSASGTLTVTDTPETLERIGRYIDHQNELLNRQVQLNVQVLSVTQSR
HHHCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCHHHHHHCCEEEEEEEEEEEHHHH
KEQFGLDWKLVYQSLNNIGASVTGNLINVANNTLSGGLSILDTATGPAEKFSGSELLIKA
HHHHCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
LSQQGNVSLVTELNRATINLTPVPFQISDQTGYIQSSSTTVTANAGTTSSMQTGIITTGL
HHCCCCEEEEEECCCEEEEECCCCEEECCCCCEEECCCEEEEECCCCCCCCCCCHHHHHH
FMSMLPYIQENGDVQLQFAFTLSDKPIIQPFFSRDGNTRNDTPAFKLRSLTQTVNLRAGQ
HHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
TLVLTGADQLDTVANKQGAFTPSNFWWGGGHNGLETRTTLVILITPVLLR
EEEEECCHHHHHHHCCCCCCCCCCCEECCCCCCCCHHEEEEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8626330; 10496929; 8932312 [H]