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The map label for this gene is btuF

Identifier: 153946963

GI number: 153946963

Start: 3738868

End: 3739710

Strand: Direct

Name: btuF

Synonym: YpsIP31758_3326

Alternate gene names: 153946963

Gene position: 3738868-3739710 (Clockwise)

Preceding gene: 153949777

Following gene: 153947152

Centisome position: 79.16

GC content: 53.5

Gene sequence:

>843_bases
ATGATGCCACTGGGTCTTTTTCCTCTGCCACGCGCAGCAGTAGTGTTGCTAATTAGCCTGCTAACACTCCCAGCACAGGC
TGCCGAACGGGTTATCAGTTTATCGCCCAGTACCACGGAACTGGCTTATGCTGCAGGTTTGGGCGATAAGTTGGTCGCGG
TCAGTGCGTACTCTGATTACCCGGAATCGGCTAAAAAGTTAGAACATGTGGCGTCGTGGCAAGGCATTAACGTTGAGCGT
ATTCTGGCACTAAAACCCGATCTGATCCTCGCCTGGCGTGGAGGTAATCCACAACGGCCGCTGGATCAATTAGCGGCGTT
GGGTATTCCGATTTTCTACTCTGACCCCACGCATATCGATCAGATTGCCAGCGATCTGGATAAGCTAGCGCAATACAGCC
CGCACCCCGAGCAGGCACATCAGGCCGCTGAACAATTCCGTCAACACGTCAACACACTGCGTGATCGCTATGCCCGCAGT
CAGCCCAAACGTACCTTTTTGCAATTCGGTACTCAGCCACTGTTTACCAGCTCCGGGCACACCTTACAGAGTGAAGTCGT
TTCACTCTGTGGTGGAGAAAATATTTTTGCCGACAGCCGGGTTCCCTGGCCACAAGTCAGTCGTGAGCAAGTGATGACAC
GTAAGCCACAGGTCATCGTGGTAAGCGGTACGCAGTCGCAAGTCGATAACGTCTCCGCCTTTTGGTTGCCTCAGCTTGTG
GTACCTGTTATCGCCCTGAATGAAGACTGGTTTAATCGCGCCAGCCCACGTATCCTGCTGGCCGCTCAACAGCTGTGCCA
ACAAATGGCCAGCATTCCAACCCCTGTCGCGGAGTCACATTAA

Upstream 100 bases:

>100_bases
AAGCATCTCACCTAAGCTTTGAAGAGTTCTTGGTGGTGGCGGCGAAACAATCGACCCTAATGATCGAAGCCATGTTAACC
ACCTTGGCGCAACGCGGTTA

Downstream 100 bases:

>100_bases
TGCTGGTTTATTGGCTGGATATCTTAGGTACTGCGGTATTTGCTATCTCTGGTGTATTACTGGCAGGAAAACTACGTATG
GATCCATTTGGCGTACTGGT

Product: vitamin B12-transporter protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MMPLGLFPLPRAAVVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDYPESAKKLEHVASWQGINVER
ILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHIDQIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARS
QPKRTFLQFGTQPLFTSSGHTLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH

Sequences:

>Translated_280_residues
MMPLGLFPLPRAAVVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDYPESAKKLEHVASWQGINVER
ILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHIDQIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARS
QPKRTFLQFGTQPLFTSSGHTLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH
>Mature_280_residues
MMPLGLFPLPRAAVVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDYPESAKKLEHVASWQGINVER
ILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHIDQIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARS
QPKRTFLQFGTQPLFTSSGHTLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain

Homologues:

Organism=Escherichia coli, GI1786353, Length=260, Percent_Identity=54.6153846153846, Blast_Score=292, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BTUF_YERP3 (A7FM05)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402283.1
- ProteinModelPortal:   A7FM05
- SMR:   A7FM05
- STRING:   A7FM05
- GeneID:   5386322
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3326
- eggNOG:   COG0614
- HOGENOM:   HBG708630
- OMA:   YDAASDY
- ProtClustDB:   PRK03379
- BioCyc:   YPSE349747:YPSIP31758_3326-MONOMER
- HAMAP:   MF_01000
- InterPro:   IPR002491

Pfam domain/function: PF01497 Peripla_BP_2

EC number: NA

Molecular weight: Translated: 30842; Mature: 30842

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50983 FE_B12_PBP

Important sites: BINDING 57-57

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLGLFPLPRAAVVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDY
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCC
PESAKKLEHVASWQGINVERILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHID
HHHHHHHHHHHHCCCCCHHHEEEECCCEEEEECCCCCCCHHHHHHHHCCCEEECCCHHHH
QIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARSQPKRTFLQFGTQPLFTSSGH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
TLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
HHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHH
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMPLGLFPLPRAAVVLLISLLTLPAQAAERVISLSPSTTELAYAAGLGDKLVAVSAYSDY
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCC
PESAKKLEHVASWQGINVERILALKPDLILAWRGGNPQRPLDQLAALGIPIFYSDPTHID
HHHHHHHHHHHHCCCCCHHHEEEECCCEEEEECCCCCCCHHHHHHHHCCCEEECCCHHHH
QIASDLDKLAQYSPHPEQAHQAAEQFRQHVNTLRDRYARSQPKRTFLQFGTQPLFTSSGH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
TLQSEVVSLCGGENIFADSRVPWPQVSREQVMTRKPQVIVVSGTQSQVDNVSAFWLPQLV
HHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHH
VPVIALNEDWFNRASPRILLAAQQLCQQMASIPTPVAESH
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA