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The map label for this gene is gacS [H]

Identifier: 153937508

GI number: 153937508

Start: 591626

End: 592873

Strand: Direct

Name: gacS [H]

Synonym: CLC_0580

Alternate gene names: 153937508

Gene position: 591626-592873 (Clockwise)

Preceding gene: 153936357

Following gene: 153936518

Centisome position: 15.73

GC content: 23.8

Gene sequence:

>1248_bases
ATGGTAAAGTATGGGGATATAAATGCTTTGAATTTATTTGTGGGGGAGTTAAATAACGTTTCCATATTCATTTTGGATAA
AGATTTTCATTATATAAAATTAAATAATAGTCATAAAAAGTTTATGGAAAAATTATTAGGGATTCATGTAGAAGTGGGTA
TGAATATATTAGAAATAATTGAAGAATACTGTCCCCTAAACATTAGTGATAATTTAATATTGAGAATAAAAGAAAAAATG
AAGAGTGCATTAAAGGGCAATAAAATTATAACAAATGAAGAAGTTTTAATTGATGGTAGTAAAGTAGAATTTTATGAAAT
AGAGATAATTCCTTTAAAGGATGAAGATAACAATATATTAGGAGTAGGTGCATGTTGCTTAAATATAACAGAGAATGTAA
ATATAATGATAGAAGTATTAAAAACTAAATTAGATTTAACCTATAAAAAACATTTAAAAAATAGTGAATTATTAAATGGG
GAAAATAGTAAGGAAAAGATATTTAAAATATTGATAGCAGAAGATAATAATGTGAATCAAATAGTAATGAGTAAATTAAT
TGAATTAAAGGGATGGGTGGCAAAAACAGTTTCTAACGGGAAGGAGTCCATAATAGCTTTAGAAAAAGACAACTATGATT
TAATCTTTATGGATATATCAATGCCCATAATGAATGGACTAGATGCAGCAAGTATAATTAAAAAAAATCCTAAATGGAGT
AAAATACCTATAGTAGCTTTAACAGCATACGACTCTTTAGAGCAAAAGAAAAAGTTTAAATCATTAGGAATGGATGATTA
TCTAAGTAAACCTATAGATTCAAATAAGTTGCAATACATAATAGATAAACATTTAAATAATAATATACAAATTAAGAATA
ATAAAGTAAAGGTAAATAATAAAGAAATATATAGTAGTTTTGAACGATTAGAAAAAAATTTAGATGGTAATAAAGAGCTA
GTAATAGAATTGGCTCATAAAATAATAAAATTATTTTCTAAAGAACAAATGAATGAAATTATGAGGTTATCAAGAGATGG
AGATATAGAAAATTTAAGAAATTTAATACATAAATTAAAGGGGGCCTCCTCAAATTTTGATTTATATAAGGTAAGAAAAT
TGCTAAATGAAATCAAGGAAAGAGCTATAGTAGGTGATATTGAACATATTTATAAATTGGTAGAAAAAATAACAATAAAT
ATGAATAAATTAGAGAAAGATTTGATAGATTATAGTAAAAATAAATAA

Upstream 100 bases:

>100_bases
TTTTATAATATATGAATATGTATAAGTTTTCCGTAGAAACTTCGAAAATCATAATAGTATGCTTAAAATAATAATATATT
TATAGGGAAGGATAAAATTT

Downstream 100 bases:

>100_bases
ATTTATAAAAATATCTTTAATCTAGTTGAAATTTGGGAATTAATGATATAAAATTAGAATATAAGCTAATATTAACAATA
TATGTGGAGAATAAGTATTT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEIIEEYCPLNISDNLILRIKEKM
KSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNILGVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNG
ENSKEKIFKILIAEDNNVNQIVMSKLIELKGWVAKTVSNGKESIIALEKDNYDLIFMDISMPIMNGLDAASIIKKNPKWS
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIKNNKVKVNNKEIYSSFERLEKNLDGNKEL
VIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLKGASSNFDLYKVRKLLNEIKERAIVGDIEHIYKLVEKITIN
MNKLEKDLIDYSKNK

Sequences:

>Translated_415_residues
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEIIEEYCPLNISDNLILRIKEKM
KSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNILGVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNG
ENSKEKIFKILIAEDNNVNQIVMSKLIELKGWVAKTVSNGKESIIALEKDNYDLIFMDISMPIMNGLDAASIIKKNPKWS
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIKNNKVKVNNKEIYSSFERLEKNLDGNKEL
VIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLKGASSNFDLYKVRKLLNEIKERAIVGDIEHIYKLVEKITIN
MNKLEKDLIDYSKNK
>Mature_415_residues
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEIIEEYCPLNISDNLILRIKEKM
KSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNILGVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNG
ENSKEKIFKILIAEDNNVNQIVMSKLIELKGWVAKTVSNGKESIIALEKDNYDLIFMDISMPIMNGLDAASIIKKNPKWS
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIKNNKVKVNNKEIYSSFERLEKNLDGNKEL
VIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLKGASSNFDLYKVRKLLNEIKERAIVGDIEHIYKLVEKITIN
MNKLEKDLIDYSKNK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=266, Percent_Identity=24.4360902255639, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI48994928, Length=218, Percent_Identity=27.9816513761468, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082012, Length=110, Percent_Identity=32.7272727272727, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=137, Percent_Identity=35.036496350365, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322000, Length=142, Percent_Identity=32.3943661971831, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47841; Mature: 47841

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEII
CCEECCCCEEEEEEECCCCEEEEEEECCEEEEEECCCHHHHHHHHHCEEHHHCCHHHHHH
EEYCPLNISDNLILRIKEKMKSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNIL
HHHCCCCCCCCHHEEEHHHHHHHHCCCEEEECCEEEEECCEEEEEEEEEEEECCCCCCEE
GVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNGENSKEKIFKILIAEDNNVNQ
EEEHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCHHH
IVMSKLIELKGWVAKTVSNGKESIIALEKDNYDLIFMDISMPIMNGLDAASIIKKNPKWS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCHHHCCCCHHHHHHCCCCCC
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIKNNKVKVNN
CCCEEEEECHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEEECC
KEIYSSFERLEKNLDGNKELVIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GASSNFDLYKVRKLLNEIKERAIVGDIEHIYKLVEKITINMNKLEKDLIDYSKNK
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVKYGDINALNLFVGELNNVSIFILDKDFHYIKLNNSHKKFMEKLLGIHVEVGMNILEII
CCEECCCCEEEEEEECCCCEEEEEEECCEEEEEECCCHHHHHHHHHCEEHHHCCHHHHHH
EEYCPLNISDNLILRIKEKMKSALKGNKIITNEEVLIDGSKVEFYEIEIIPLKDEDNNIL
HHHCCCCCCCCHHEEEHHHHHHHHCCCEEEECCEEEEECCEEEEEEEEEEEECCCCCCEE
GVGACCLNITENVNIMIEVLKTKLDLTYKKHLKNSELLNGENSKEKIFKILIAEDNNVNQ
EEEHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCHHH
IVMSKLIELKGWVAKTVSNGKESIIALEKDNYDLIFMDISMPIMNGLDAASIIKKNPKWS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCHHHCCCCHHHHHHCCCCCC
KIPIVALTAYDSLEQKKKFKSLGMDDYLSKPIDSNKLQYIIDKHLNNNIQIKNNKVKVNN
CCCEEEEECHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEEECC
KEIYSSFERLEKNLDGNKELVIELAHKIIKLFSKEQMNEIMRLSRDGDIENLRNLIHKLK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GASSNFDLYKVRKLLNEIKERAIVGDIEHIYKLVEKITINMNKLEKDLIDYSKNK
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]