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The map label for this gene is bioD

Identifier: 153937439

GI number: 153937439

Start: 2315956

End: 2316639

Strand: Reverse

Name: bioD

Synonym: CLC_2182

Alternate gene names: 153937439

Gene position: 2316639-2315956 (Counterclockwise)

Preceding gene: 153934583

Following gene: 153936290

Centisome position: 61.6

GC content: 29.39

Gene sequence:

>684_bases
ATGGCAAGAGGAATATTTATAACAGCCACAGGAACTGATATTGGAAAAACTTACGTAACGGCTTTAATTATTAAGAGATT
AAGAGAAACTAATATAAATTGTGGATACTATAAAGCTGCTCTAAGCGGGGCAGAGAGAAGAGATGGTAAACTTATTGCAG
GAGATGCTAACTATGTATATAATATTGCCAACATAAAGGGAGATCCTAATGATGCAGTGAGTTATATATTTCAGCAAGCA
GTTTCTCCTCATTTAGCAGCAAAGTTAAATAATGTAGAAATATCTATGGAGAGGATAAAAAAAGATTTTTACTCTATTAA
AAATAAATATGATTATATAACTGTAGAAGGTAGTGGAGGAATAGTTTGTCCTATTTCCACAGGTAAAGAAAGAATAATGC
TTCACAATATCATAAAGATCTTTAAACTACCGGCTATTGTTGTAGCAGATGCAGGTCTTGGCACCATTAACAGTACAATT
TTAACCCTTCAATATATGAAAGAGAAAAATATATCTGTAAAAATGATTTTATTAAACAATTATAACCATAAAGATATAAT
TCATATAGAAAATAAGGGATACCTATCAGATAATTTACTAATTCCAGTTTATACATGTAATAAGAATGCTAATAATTTAG
AAATTCCAGTAGAAAAACTAATTGAAATCTATGAGGAGATATAA

Upstream 100 bases:

>100_bases
GAGTAAATGGAGCAATTTCTGGAGATATGCTAACTACTTTAGGAATTGAAACTTCTGAGGACATTAAAATGATAAAGAAT
TTAGGATTTGAGGTATAAAT

Downstream 100 bases:

>100_bases
TTTAGCATTTAACACTATTGACAAACAATATTAATATGGTTAGTGTAATATTAATATTGTAATAAATTTAGAATGTTATA
GATTTGATGGGGAAATGGAG

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MARGIFITATGTDIGKTYVTALIIKRLRETNINCGYYKAALSGAERRDGKLIAGDANYVYNIANIKGDPNDAVSYIFQQA
VSPHLAAKLNNVEISMERIKKDFYSIKNKYDYITVEGSGGIVCPISTGKERIMLHNIIKIFKLPAIVVADAGLGTINSTI
LTLQYMKEKNISVKMILLNNYNHKDIIHIENKGYLSDNLLIPVYTCNKNANNLEIPVEKLIEIYEEI

Sequences:

>Translated_227_residues
MARGIFITATGTDIGKTYVTALIIKRLRETNINCGYYKAALSGAERRDGKLIAGDANYVYNIANIKGDPNDAVSYIFQQA
VSPHLAAKLNNVEISMERIKKDFYSIKNKYDYITVEGSGGIVCPISTGKERIMLHNIIKIFKLPAIVVADAGLGTINSTI
LTLQYMKEKNISVKMILLNNYNHKDIIHIENKGYLSDNLLIPVYTCNKNANNLEIPVEKLIEIYEEI
>Mature_226_residues
ARGIFITATGTDIGKTYVTALIIKRLRETNINCGYYKAALSGAERRDGKLIAGDANYVYNIANIKGDPNDAVSYIFQQAV
SPHLAAKLNNVEISMERIKKDFYSIKNKYDYITVEGSGGIVCPISTGKERIMLHNIIKIFKLPAIVVADAGLGTINSTIL
TLQYMKEKNISVKMILLNNYNHKDIIHIENKGYLSDNLLIPVYTCNKNANNLEIPVEKLIEIYEEI

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=203, Percent_Identity=23.1527093596059, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324385, Length=171, Percent_Identity=29.2397660818713, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_CLOB1 (A7FVS5)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384512.1
- ProteinModelPortal:   A7FVS5
- STRING:   A7FVS5
- GeneID:   5395801
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2199
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   LNNYNHE
- ProtClustDB:   PRK00090
- BioCyc:   CBOT441770:CLB_2199-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 25364; Mature: 25233

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGIFITATGTDIGKTYVTALIIKRLRETNINCGYYKAALSGAERRDGKLIAGDANYVY
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEECCCEEE
NIANIKGDPNDAVSYIFQQAVSPHLAAKLNNVEISMERIKKDFYSIKNKYDYITVEGSGG
EEEECCCCCHHHHHHHHHHHCCCHHHHEECCEEEHHHHHHHHHHHHCCCCEEEEEECCCC
IVCPISTGKERIMLHNIIKIFKLPAIVVADAGLGTINSTILTLQYMKEKNISVKMILLNN
EEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHEEEEEEHHCCCCEEEEEEEEC
YNHKDIIHIENKGYLSDNLLIPVYTCNKNANNLEIPVEKLIEIYEEI
CCCCEEEEECCCCEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
ARGIFITATGTDIGKTYVTALIIKRLRETNINCGYYKAALSGAERRDGKLIAGDANYVY
CCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEECCCEEE
NIANIKGDPNDAVSYIFQQAVSPHLAAKLNNVEISMERIKKDFYSIKNKYDYITVEGSGG
EEEECCCCCHHHHHHHHHHHCCCHHHHEECCEEEHHHHHHHHHHHHCCCCEEEEEECCCC
IVCPISTGKERIMLHNIIKIFKLPAIVVADAGLGTINSTILTLQYMKEKNISVKMILLNN
EEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHEEEEEEHHCCCCEEEEEEEEC
YNHKDIIHIENKGYLSDNLLIPVYTCNKNANNLEIPVEKLIEIYEEI
CCCCEEEEECCCCEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA