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The map label for this gene is bioB

Identifier: 153934583

GI number: 153934583

Start: 2316642

End: 2317598

Strand: Reverse

Name: bioB

Synonym: CLC_2183

Alternate gene names: 153934583

Gene position: 2317598-2316642 (Counterclockwise)

Preceding gene: 153935151

Following gene: 153937439

Centisome position: 61.63

GC content: 30.62

Gene sequence:

>957_bases
ATGAGTAATATAATTAAATATAAGAAAAAAATTTTAAATGGAGATTTACTAACAAAAGAAGAAGTAGAAGAGCTTTTAGA
GGAAGATATTACAGATCTTGCAGCAACAGCTAATGAAATTAGAGAATCTCTATGTGGAAATAAATTCGACTTATGTACAA
TTATAAATGGGAAGAGTGGAAGGTGCCAAGAAAACTGTAAATATTGTGCTCAATCTGCTCACTTTGACACAGATATAATA
GAGTACAACATCTTAAATAGTGATAGAATTATAAATAGTGCAATATCAAATTATAATAAAGGAGTACATCGTTTTTCAGT
AGTAACCTCAGGTCGTGCACTAAATAATAATGAAGTAGATACATTGTGTAAAACCTATTCAAAATTAAAAGAAACATGTT
CTATTAGACTTTGCGCATCCCATGGTTTATTAAAATATGAAGATCTAAAGAGGTTAAAAGATTCTGGAGTAACGCGTTAT
CACAATAATTTGGAAACCTCAAGAAAATTTTTTACAAAGATATGCACTACCCATAAATATGATGACAAAATTGAAACTAT
AAAAAATGCAAAGAAAGCTGGTATTGAGATATGTAGTGGTGGAATTATTGGTCTTGGAGAAACCATGGAGGACCGAATCG
ATATGGCTTTTACTTTAAGAGAGCTTTCAGTTGAAAGTGTTCCAGTTAATATTCTTAATCCTATTAAAGGAACACCACTT
GAAAATCAAGAAATTTTATCCTATGAAGAAATAATTAAAACTCTAGCACTTTTCCGTTTTATTTTACCAACAGTGCAAAT
AAGGTTAGCTGGAGGTCGTACTATTATAAGTGATAAGGGGAAAAAGGCCTTAGAAAGTGGAGTAAATGGAGCAATTTCTG
GAGATATGCTAACTACTTTAGGAATTGAAACTTCTGAGGACATTAAAATGATAAAGAATTTAGGATTTGAGGTATAA

Upstream 100 bases:

>100_bases
TTATCTTGTTTTCCATTAGACATGCCTGGTGACATATTTATATGCATAATGAGTGCATTATTTGCTAATAAAATTAACCC
AATTTTAAGGAGAGAAAGAA

Downstream 100 bases:

>100_bases
ATATGGCAAGAGGAATATTTATAACAGCCACAGGAACTGATATTGGAAAAACTTACGTAACGGCTTTAATTATTAAGAGA
TTAAGAGAAACTAATATAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSGRCQENCKYCAQSAHFDTDII
EYNILNSDRIINSAISNYNKGVHRFSVVTSGRALNNNEVDTLCKTYSKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRY
HNNLETSRKFFTKICTTHKYDDKIETIKNAKKAGIEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTLGIETSEDIKMIKNLGFEV

Sequences:

>Translated_318_residues
MSNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSGRCQENCKYCAQSAHFDTDII
EYNILNSDRIINSAISNYNKGVHRFSVVTSGRALNNNEVDTLCKTYSKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRY
HNNLETSRKFFTKICTTHKYDDKIETIKNAKKAGIEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTLGIETSEDIKMIKNLGFEV
>Mature_317_residues
SNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSGRCQENCKYCAQSAHFDTDIIE
YNILNSDRIINSAISNYNKGVHRFSVVTSGRALNNNEVDTLCKTYSKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRYH
NNLETSRKFFTKICTTHKYDDKIETIKNAKKAGIEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPLE
NQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTLGIETSEDIKMIKNLGFEV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=30.4918032786885, Blast_Score=171, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=33.4394904458599, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CLOB1 (A7FVS6)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384513.1
- ProteinModelPortal:   A7FVS6
- SMR:   A7FVS6
- STRING:   A7FVS6
- GeneID:   5397839
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2200
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK972180
- BioCyc:   CBOT441770:CLB_2200-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35506; Mature: 35375

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSG
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
RCQENCKYCAQSAHFDTDIIEYNILNSDRIINSAISNYNKGVHRFSVVTSGRALNNNEVD
CCHHHHHHHHHHCCCCCHHEEEEECCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCCHHH
TLCKTYSKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRYHNNLETSRKFFTKICTTHKY
HHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
DDKIETIKNAKKAGIEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
CHHHHHHHHHHHHCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTL
CCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHHHHHCCCCCCCCCHHHHC
GIETSEDIKMIKNLGFEV
CCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNIIKYKKKILNGDLLTKEEVEELLEEDITDLAATANEIRESLCGNKFDLCTIINGKSG
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
RCQENCKYCAQSAHFDTDIIEYNILNSDRIINSAISNYNKGVHRFSVVTSGRALNNNEVD
CCHHHHHHHHHHCCCCCHHEEEEECCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCCHHH
TLCKTYSKLKETCSIRLCASHGLLKYEDLKRLKDSGVTRYHNNLETSRKFFTKICTTHKY
HHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
DDKIETIKNAKKAGIEICSGGIIGLGETMEDRIDMAFTLRELSVESVPVNILNPIKGTPL
CHHHHHHHHHHHHCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
ENQEILSYEEIIKTLALFRFILPTVQIRLAGGRTIISDKGKKALESGVNGAISGDMLTTL
CCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHHHHHCCCCCCCCCHHHHC
GIETSEDIKMIKNLGFEV
CCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA