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The map label for this gene is pepT

Identifier: 153936996

GI number: 153936996

Start: 520740

End: 521966

Strand: Direct

Name: pepT

Synonym: CLC_0513

Alternate gene names: 153936996

Gene position: 520740-521966 (Clockwise)

Preceding gene: 153936163

Following gene: 153937479

Centisome position: 13.85

GC content: 30.89

Gene sequence:

>1227_bases
ATGAAAGATGTATTAGAAAGATTCTTAGGGTATATAAAAGTAGATACCCAATCATCAGAGGAATCTGATACTGTGCCAAC
TACTAAAACTCAATTAGAGTTTGCAAAAAAATTAGGAGAAGAATTAAAGGCTATAGGACTTAAAGATGTATCTGTAGATG
AAAACGGATATGTTATGGCTACTCTAGAATCAAACATAGATAAAAAAGTGCCTACAATTGGCTTTATAGCACATATGGAT
ACAAGTCCAGACTTATCAGGAACTAATATTAATCCTAGAATTGTAGAGAAATATGATGGACAAGATATAGTTTTAAACAA
AGAAAAAAACATAGTATTGAAGATTAATGAATTTCCAGAAATATTGGAATACAAGGGACAAGACATAGTAGTAACAGATG
GAAATACTCTTTTGGGAGCAGATGACAAAGCTGGAATAGCAGAGATAATTACGGCTATGGAATATCTAATAAATCATCCA
GAAATAAAACATGGAACTATAAAAGTTGGTTTTACCCCAGATGAAGAAGTGGGTAAAGGGGCAGACCACTTTGATGTTAA
AAAATTTGGAGCAGATTTAGCCTATACTTTAGATGGTGGCGGTATCGGAGAGTTAGAATGTGAAACTTTTAATGCTGCAA
AAGCTAAAGTTATTATAGAAGGAAGAAATGTTCATCCAGGTTCTGCAAAAAATAAAATGACAAATGCAGTATTAGTAGCT
AATAAATTTATAAATATGCTTCCAGAAAATGAAGTGCCAGAAAGAACAGAAGGATATGAAGGATTTTTCCATCTATTATC
TGTAAAGAGTGAAGTAGAAACAGCAGAACTAAATTATATTATAAGAGATTTTGATAGAAAAAAATTTGAAGAAAGAAAAG
AACAAATAAAAGAAGTTGGTAAAAAAATAAATGAAGAATACAATAAAGAAATAGTATGTGTAAAAGTAGAAGATCAATAT
TATAATATGAAAGAAAAAATAGATGAAGTAAAATATGTGGTAGATATAGCTTATGATGCTATGAAAGCTATTGACATTGA
ACCAATATTAGTGCCTATAAGAGGTGGTACAGATGGTTCAAGACTATCTTTTATGGGATTACCAACTCCAAATTTATTTG
CAGGTGGACATAATTTCCACGGAAGATTTGAGTTTGTTCCAGTTCTATCAATGGAAAAAGCAGCAGAACTTGTGGTTAAA
ATAGCTGAACTATATGCTAATAGATAA

Upstream 100 bases:

>100_bases
GGAGATGTTTGCATAGAACAATAGTATATTGTAGAATATAAGTAAACAATTACAAAAAATATAAATAAAAACCAAAATTT
GTTTTAAAGGGGGATCATTA

Downstream 100 bases:

>100_bases
TAAGTTTTTTAAAATTTAACATTGTTTTTATTAAATATAAAATATTCATAGAAAATGTCTTAAAATAAAAAGATTTAATT
TTAAGATTGCTAGAATAGAA

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDENGYVMATLESNIDKKVPTIGFIAHMD
TSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPEILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHP
EIKHGTIKVGFTPDEEVGKGADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
NKFINMLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVGKKINEEYNKEIVCVKVEDQY
YNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGSRLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVK
IAELYANR

Sequences:

>Translated_408_residues
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDENGYVMATLESNIDKKVPTIGFIAHMD
TSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPEILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHP
EIKHGTIKVGFTPDEEVGKGADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
NKFINMLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVGKKINEEYNKEIVCVKVEDQY
YNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGSRLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVK
IAELYANR
>Mature_408_residues
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDENGYVMATLESNIDKKVPTIGFIAHMD
TSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPEILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHP
EIKHGTIKVGFTPDEEVGKGADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
NKFINMLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVGKKINEEYNKEIVCVKVEDQY
YNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGSRLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVK
IAELYANR

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=409, Percent_Identity=53.3007334963325, Blast_Score=440, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_CLOB1 (A7FPG2)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382831.1
- ProteinModelPortal:   A7FPG2
- SMR:   A7FPG2
- STRING:   A7FPG2
- GeneID:   5396712
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0480
- eggNOG:   COG2195
- HOGENOM:   HBG288638
- OMA:   HMDTAPD
- ProtClustDB:   PRK05469
- BioCyc:   CBOT441770:CLB_0480-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 45609; Mature: 45609

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 80-80 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDENGYVMA
CHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEE
TLESNIDKKVPTIGFIAHMDTSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPE
EECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEECCCCH
ILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHPEIKHGTIKVGFTPDEEVGKG
HHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHCCC
ADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
CCCCHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHEEHH
NKFINMLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKINEEYNKEIVCVKVEDQYYNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGS
HHHHHHHCCCEEEEEECCHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
RLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVKIAELYANR
EEEEEECCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDENGYVMA
CHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEE
TLESNIDKKVPTIGFIAHMDTSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPE
EECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEECCCCH
ILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHPEIKHGTIKVGFTPDEEVGKG
HHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHCCC
ADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
CCCCHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHEEHH
NKFINMLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKINEEYNKEIVCVKVEDQYYNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGS
HHHHHHHCCCEEEEEECCHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
RLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVKIAELYANR
EEEEEECCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA