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The map label for this gene is ldhA-1 [C]

Identifier: 153936500

GI number: 153936500

Start: 3360843

End: 3361847

Strand: Direct

Name: ldhA-1 [C]

Synonym: CLC_3225

Alternate gene names: 153936500

Gene position: 3360843-3361847 (Clockwise)

Preceding gene: 153935553

Following gene: 153937030

Centisome position: 89.37

GC content: 32.54

Gene sequence:

>1005_bases
ATGAAAATACTAATGTACAGCGTGCGTGAGCACGAAAAACCAGCTATAAAAAAATGGCTAGAAGCAAATCCTGGAGTTCA
AATTGATTTATGTAATAATGCTCTTTCTGAAGATACTGTCTGTAAGGCAAAAGAATATGATGGAATTGCAATTCAGCAAA
CAAATAGTATAGGTGGAAAAGCTGTTTATTCTACCCTTAAAGAATATGGAATTAAACAAATAGCTTCAAGAACTGCTGGG
GTAGATATGATTGATTTAAAGATGGCTTCAGACAGCAATATTCTTGTTACCAATGTTCCAGCTTATTCTCCAAATGCTAT
TGCAGAACTTGCTGTAACTCATACAATGAATTTGCTGAGAAACATTAAAACTTTAAATAAAAGAATAGCTTATGGTGATT
ATCGTTGGTCAGCTGACCTTATAGCTAGAGAAGTAAGATCTGTAACAGTTGGTGTAGTAGGTACAGGCAAAATAGGTAGA
ACTTCTGCCAAGCTTTTTAAAGGATTAGGGGCAAATGTTATAGGTTATGATGCTTATCCAGACAAAAAATTAGAAGAAAA
TAATCTCCTTACTTACAAAGAATCATTGGAGGATCTTTTAAGAGAAGCTGATGTTGTAACTCTGCACACACCTCTTCTGG
AAAGTACTAAATATATGATAAATAAGAATAATTTGAAATATATGAAACCAGATGCTTTCATAGTAAATACTGGTCGTGGT
GGAATTATCAATACTGAAGATCTTATTGAAGCCTTAGAACAAAATAAAATAGCTGGGGCTGCACTTGATACTTTTGAAAA
TGAAGGATTATTCTTAAATAAAGTAGTTGATCCAACTAAACTTCCTGACTCTCAACTTGATAAATTATTAAAAATGGATC
AAGTTTTAATAACTCATCATGTTGGTTTCTTCACTACTACTGCAGTTCAAAACATAGTTGATACTAGCTTAGATAGTGTT
GTAGAGGTTTTAAAGACTAACAATAGTGTAAACAAGGTAAATTAA

Upstream 100 bases:

>100_bases
CAAATTAATATTGTACATCAGAAGGCTTATGGCTTTTAATATATTTTTTATTAACTTACACTTTAAAAATATATTTAAAT
TCTAAAGGGGGAAATATTTT

Downstream 100 bases:

>100_bases
AAATTATCTTTTTTAAAAAATGAAAGCGTTTTCAGGATTTATTAATCTTAAAGTAAATAGAAAACGCTTTCTATTACCAT
TTTATTATTTTCTTAAATTT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; D-specific D-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNNALSEDTVCKAKEYDGIAIQQTNSIGGKAVYSTLKEYGIKQIASRTAG
VDMIDLKMASDSNILVTNVPAYSPNAIAELAVTHTMNLLRNIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGR
TSAKLFKGLGANVIGYDAYPDKKLEENNLLTYKESLEDLLREADVVTLHTPLLESTKYMINKNNLKYMKPDAFIVNTGRG
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDSQLDKLLKMDQVLITHHVGFFTTTAVQNIVDTSLDSV
VEVLKTNNSVNKVN

Sequences:

>Translated_334_residues
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNNALSEDTVCKAKEYDGIAIQQTNSIGGKAVYSTLKEYGIKQIASRTAG
VDMIDLKMASDSNILVTNVPAYSPNAIAELAVTHTMNLLRNIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGR
TSAKLFKGLGANVIGYDAYPDKKLEENNLLTYKESLEDLLREADVVTLHTPLLESTKYMINKNNLKYMKPDAFIVNTGRG
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDSQLDKLLKMDQVLITHHVGFFTTTAVQNIVDTSLDSV
VEVLKTNNSVNKVN
>Mature_334_residues
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNNALSEDTVCKAKEYDGIAIQQTNSIGGKAVYSTLKEYGIKQIASRTAG
VDMIDLKMASDSNILVTNVPAYSPNAIAELAVTHTMNLLRNIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGR
TSAKLFKGLGANVIGYDAYPDKKLEENNLLTYKESLEDLLREADVVTLHTPLLESTKYMINKNNLKYMKPDAFIVNTGRG
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDSQLDKLLKMDQVLITHHVGFFTTTAVQNIVDTSLDSV
VEVLKTNNSVNKVN

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=248, Percent_Identity=33.4677419354839, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557497, Length=248, Percent_Identity=33.4677419354839, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI145580578, Length=248, Percent_Identity=30.6451612903226, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4557499, Length=248, Percent_Identity=30.6451612903226, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI23308577, Length=255, Percent_Identity=28.6274509803922, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI145580575, Length=196, Percent_Identity=33.6734693877551, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=26.5822784810127, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1787645, Length=335, Percent_Identity=31.6417910447761, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789279, Length=240, Percent_Identity=32.5, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082289, Length=267, Percent_Identity=25.8426966292135, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17532191, Length=299, Percent_Identity=28.4280936454849, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=276, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322116, Length=319, Percent_Identity=27.8996865203762, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=319, Percent_Identity=26.9592476489028, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=229, Percent_Identity=31.0043668122271, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=210, Percent_Identity=29.0476190476191, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324980, Length=75, Percent_Identity=38.6666666666667, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646446, Length=314, Percent_Identity=30.8917197452229, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646448, Length=314, Percent_Identity=30.8917197452229, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646452, Length=314, Percent_Identity=30.8917197452229, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646450, Length=314, Percent_Identity=30.8917197452229, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI62472511, Length=268, Percent_Identity=33.2089552238806, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19921140, Length=240, Percent_Identity=32.0833333333333, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574286, Length=240, Percent_Identity=30.4166666666667, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI45552429, Length=242, Percent_Identity=31.8181818181818, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585514, Length=242, Percent_Identity=31.8181818181818, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574282, Length=242, Percent_Identity=31.8181818181818, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574284, Length=239, Percent_Identity=31.7991631799163, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45551003, Length=242, Percent_Identity=31.8181818181818, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28571528, Length=234, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585516, Length=268, Percent_Identity=24.2537313432836, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36880; Mature: 36880

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNNALSEDTVCKAKEYDGIAIQQTNSIGGK
CEEEEEHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCCCCEEEEECCCCCHH
AVYSTLKEYGIKQIASRTAGVDMIDLKMASDSNILVTNVPAYSPNAIAELAVTHTMNLLR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGRTSAKLFKGLGANVIGYDAYP
HHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCCEEEECCCC
DKKLEENNLLTYKESLEDLLREADVVTLHTPLLESTKYMINKNNLKYMKPDAFIVNTGRG
CCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHEEECCCCEEECCCEEEEECCCC
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDSQLDKLLKMDQVLITHH
CCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VGFFTTTAVQNIVDTSLDSVVEVLKTNNSVNKVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILMYSVREHEKPAIKKWLEANPGVQIDLCNNALSEDTVCKAKEYDGIAIQQTNSIGGK
CEEEEEHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCCCCEEEEECCCCCHH
AVYSTLKEYGIKQIASRTAGVDMIDLKMASDSNILVTNVPAYSPNAIAELAVTHTMNLLR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NIKTLNKRIAYGDYRWSADLIAREVRSVTVGVVGTGKIGRTSAKLFKGLGANVIGYDAYP
HHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCCEEEECCCC
DKKLEENNLLTYKESLEDLLREADVVTLHTPLLESTKYMINKNNLKYMKPDAFIVNTGRG
CCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHEEECCCCEEECCCEEEEECCCC
GIINTEDLIEALEQNKIAGAALDTFENEGLFLNKVVDPTKLPDSQLDKLLKMDQVLITHH
CCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VGFFTTTAVQNIVDTSLDSVVEVLKTNNSVNKVN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840590; 8740366 [H]