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The map label for this gene is lexA

Identifier: 153936246

GI number: 153936246

Start: 1840161

End: 1840766

Strand: Direct

Name: lexA

Synonym: CLC_1737

Alternate gene names: 153936246

Gene position: 1840161-1840766 (Clockwise)

Preceding gene: 153936224

Following gene: 153936139

Centisome position: 48.93

GC content: 28.71

Gene sequence:

>606_bases
ATGAATAAAAGCCGAATTGACAAACAAAATGAAGTTTATAACTTTATAAAATTACAAATTAAAGAAAAAGGCTATCCTCC
TTCTGTAAGAGAAATTTGCAAGGCGGTAGGTTTAAGCTCAACTTCATCAGTGCATTTTCATCTTAAAAGATTAGAAAAAG
AAGGTTTAATAAAGAGAGATAGTAGCAAAACTAGAGCTATAGAAATTGTTGATCCTACCTCAAAAAAAGAAGTTATAAAT
GTGCCTATTGTAGGAACTATAACTGCAGGCAATCCTATTTTAGCTATAGAAAATATAGAAGATGTATTTCCATTGCCTAT
TGATTATGTTAAAAACACAAAAGATTTATTTATGCTAAAGGTATCTGGAGAAAGTATGATAGAAGCTGGAATATTGAATG
GAGATTTAGCTATTATTGAAAAAACCGATTCTGCAAATAATGGAGATATTGTGGTTGCTCTAATAGACAACGAAGCTACT
TTAAAAAGATTTTTTAAAGAAAGCTCTTATATAAGACTTCAGCCTGAAAATAAAAGTATGAAACCTATAATTTTAGAGAA
TTGTAAAGTTCTTGGAAGATTAGTAGGAATATATAGAAAGTATTAA

Upstream 100 bases:

>100_bases
ATCATAAGTTATATTATTTTATAATTTATTTGAACATTAGTTTGCAGCTTTCCATTTTTATGGTATAATTTAATTATTAT
TTAATTAATGAGGTGTGATT

Downstream 100 bases:

>100_bases
TAACATTGTTATTTGGATGAATAAATGTGTATTAAAAATAATTTACTTTTAAAGAGGATTGTTAATAAAAATAATTCCTA
GGAGTATTAAGTGAATAACT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRDSSKTRAIEIVDPTSKKEVIN
VPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLKVSGESMIEAGILNGDLAIIEKTDSANNGDIVVALIDNEAT
LKRFFKESSYIRLQPENKSMKPIILENCKVLGRLVGIYRKY

Sequences:

>Translated_201_residues
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRDSSKTRAIEIVDPTSKKEVIN
VPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLKVSGESMIEAGILNGDLAIIEKTDSANNGDIVVALIDNEAT
LKRFFKESSYIRLQPENKSMKPIILENCKVLGRLVGIYRKY
>Mature_201_residues
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRDSSKTRAIEIVDPTSKKEVIN
VPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLKVSGESMIEAGILNGDLAIIEKTDSANNGDIVVALIDNEAT
LKRFFKESSYIRLQPENKSMKPIILENCKVLGRLVGIYRKY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=201, Percent_Identity=35.8208955223881, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787431, Length=134, Percent_Identity=27.6119402985075, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_CLOB1 (A7FUK4)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384051.1
- ProteinModelPortal:   A7FUK4
- SMR:   A7FUK4
- STRING:   A7FUK4
- GeneID:   5395451
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1729
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   CBOT441770:CLB_1729-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22579; Mature: 22579

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: ACT_SITE 125-125 ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRD
CCCCCCCCHHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHEEHHHHCCCEECC
SSKTRAIEIVDPTSKKEVINVPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLK
CCCCEEEEEECCCCCCEEEECCEEEEEECCCEEEEECCCCCCCCCCHHHHCCCCEEEEEE
VSGESMIEAGILNGDLAIIEKTDSANNGDIVVALIDNEATLKRFFKESSYIRLQPENKSM
ECCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEECCCCCCC
KPIILENCKVLGRLVGIYRKY
CCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRD
CCCCCCCCHHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHEEHHHHCCCEECC
SSKTRAIEIVDPTSKKEVINVPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLK
CCCCEEEEEECCCCCCEEEECCEEEEEECCCEEEEECCCCCCCCCCHHHHCCCCEEEEEE
VSGESMIEAGILNGDLAIIEKTDSANNGDIVVALIDNEATLKRFFKESSYIRLQPENKSM
ECCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEECCCCCCC
KPIILENCKVLGRLVGIYRKY
CCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA