Click here to switch to the map view.

The map label for this gene is hemC

Identifier: 153936114

GI number: 153936114

Start: 999518

End: 1000390

Strand: Direct

Name: hemC

Synonym: CLC_0975

Alternate gene names: 153936114

Gene position: 999518-1000390 (Clockwise)

Preceding gene: 153937217

Following gene: 153935140

Centisome position: 26.58

GC content: 31.27

Gene sequence:

>873_bases
TTGAATTTTGTAATAGCCACACGAAGAAGTAAGCTTGCTCAGGTGCAAACAGAAATAATAATAGATTTATTAAATAAAAA
GCATGATATAGAATGTGAAAAATTATTAATAGAAACTGTAGGAGATAAAATATTAGAAGTATCTTTAGATAAGATAGGTG
GAAAAGGGCTATTTGTTAAAGACATAGAAGTAGCTATGTTAGAACAAAGGGCAGATGCAGCAGTACATAGCATGAAGGAT
GTACCCTATGAAATGCCAAAAGGTTTTGAGATAATAGCTATTCCAGAGCGAGAAGATGTAAGAGATGCCTTTATATCCTT
AGACAATATAAAATTTAAGGATTTAAGAAAGGGAGCTAAAATTGGTACAAGTAGTAGGAGAAGGGCAGCCCAATTAAAAC
TTCTAAGGCCAGATTTAGATATTGTACCCATAAGAGGAAATGTTCAAACTAGAATAGAAAAAATAAAAAAAGAAAATCTA
GATGGAGTAATATTAGCAGTAGCAGGCCTTAAAAGGGTAAATTTAGATCATTTAATTACAGACTATTTTGATACAAAAGA
AATGGTACCCGCAATTGGTCAAGGTGCCTTAGGTATAGAAGTTATGGAGGAGCATCCTAAAAAGGAGTTATTTAAGGATT
TAGATCATTATAATTCAAAAATATGTGTTTTGGCAGAAAGAGCTTTTATGAGGGAACTAGATGGAGATTGCCACTCAACT
ATAGGAGCTTATGCATCTATAAAGGATAATATAATGCATATAATAGGCATATTTGAAAGAAAAAATAAAATTATTAAAAA
AGAAATCACAGGAACAAAAGATCAGTATGAAAAATTAGGCATATCCCTAGCAGAACATATACTAAAAGACTAA

Upstream 100 bases:

>100_bases
GATGAAATTATAGATTTTATCTGCAGCAATGATTTTCACTTCTTTTTTGAAAAGGGGTATGGTAATCTAATCTTATCAAT
GTTTTATGGAGGTATGGATT

Downstream 100 bases:

>100_bases
AAATTTATTGTTAAAGGTTAAGAATCCTAATAATACTCATCTTAATAGAAGATGTGAGTATTATAAGTGGTATTAGTGGA
AAATAGTAATTTTGAATAAA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNFVIATRRSKLAQVQTEIIIDLLNKKHDIECEKLLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKD
VPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKLLRPDLDIVPIRGNVQTRIEKIKKENL
DGVILAVAGLKRVNLDHLITDYFDTKEMVPAIGQGALGIEVMEEHPKKELFKDLDHYNSKICVLAERAFMRELDGDCHST
IGAYASIKDNIMHIIGIFERKNKIIKKEITGTKDQYEKLGISLAEHILKD

Sequences:

>Translated_290_residues
MNFVIATRRSKLAQVQTEIIIDLLNKKHDIECEKLLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKD
VPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKLLRPDLDIVPIRGNVQTRIEKIKKENL
DGVILAVAGLKRVNLDHLITDYFDTKEMVPAIGQGALGIEVMEEHPKKELFKDLDHYNSKICVLAERAFMRELDGDCHST
IGAYASIKDNIMHIIGIFERKNKIIKKEITGTKDQYEKLGISLAEHILKD
>Mature_290_residues
MNFVIATRRSKLAQVQTEIIIDLLNKKHDIECEKLLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKD
VPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKLLRPDLDIVPIRGNVQTRIEKIKKENL
DGVILAVAGLKRVNLDHLITDYFDTKEMVPAIGQGALGIEVMEEHPKKELFKDLDHYNSKICVLAERAFMRELDGDCHST
IGAYASIKDNIMHIIGIFERKNKIIKKEITGTKDQYEKLGISLAEHILKD

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=258, Percent_Identity=36.046511627907, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI20149500, Length=258, Percent_Identity=36.046511627907, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI48994974, Length=291, Percent_Identity=44.6735395189003, Blast_Score=225, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6319996, Length=251, Percent_Identity=39.8406374501992, Blast_Score=162, Evalue=5e-41,
Organism=Drosophila melanogaster, GI20130425, Length=251, Percent_Identity=37.4501992031873, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_CLOB1 (A7FSG8)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383295.1
- ProteinModelPortal:   A7FSG8
- SMR:   A7FSG8
- STRING:   A7FSG8
- GeneID:   5397822
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0961
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   RATEVFD
- ProtClustDB:   PRK00072
- BioCyc:   CBOT441770:CLB_0961-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 32825; Mature: 32825

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVIATRRSKLAQVQTEIIIDLLNKKHDIECEKLLIETVGDKILEVSLDKIGGKGLFVK
CCEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
DIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHCCCC
IGTSSRRRAAQLKLLRPDLDIVPIRGNVQTRIEKIKKENLDGVILAVAGLKRVNLDHLIT
CCCCHHHHHHHHHEECCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCHHCCHHHHHH
DYFDTKEMVPAIGQGALGIEVMEEHPKKELFKDLDHYNSKICVLAERAFMRELDGDCHST
HHHCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHH
IGAYASIKDNIMHIIGIFERKNKIIKKEITGTKDQYEKLGISLAEHILKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFVIATRRSKLAQVQTEIIIDLLNKKHDIECEKLLIETVGDKILEVSLDKIGGKGLFVK
CCEEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
DIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHCCCC
IGTSSRRRAAQLKLLRPDLDIVPIRGNVQTRIEKIKKENLDGVILAVAGLKRVNLDHLIT
CCCCHHHHHHHHHEECCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCHHCCHHHHHH
DYFDTKEMVPAIGQGALGIEVMEEHPKKELFKDLDHYNSKICVLAERAFMRELDGDCHST
HHHCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHH
IGAYASIKDNIMHIIGIFERKNKIIKKEITGTKDQYEKLGISLAEHILKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA