Click here to switch to the map view.

The map label for this gene is cobA/hemD [H]

Identifier: 153935140

GI number: 153935140

Start: 1000526

End: 1001986

Strand: Direct

Name: cobA/hemD [H]

Synonym: CLC_0976

Alternate gene names: 153935140

Gene position: 1000526-1001986 (Clockwise)

Preceding gene: 153936114

Following gene: 153937637

Centisome position: 26.61

GC content: 30.05

Gene sequence:

>1461_bases
ATGAGTAAAGTATATTTAATTGGTGCAGGGCCAGGTGATGAAGAATTAATAACTTTAAAAGCCATAAGAGCCCTAAAAAA
ATGTACTGCAGTTATGTATGATAGATTAGCAAATGGAGAATTGTTAAAATATTTAGCACCAAATTGTGAAATTTATTATT
GTGGAAAAGAACCGGGCTGTCATTATAAAAGCCAGGATGAAATAAATAAAATGCTAGTTAAATTAGCAAAAGAAGGTCAT
ATTGTAGGAAGAATAAAAGGTGGAGATCCCTATGTATTTGGAAGAGGGGGAGAAGAAGCCTTGGATATTTTAGAAGAAAA
TATAGAATTTGAAGTTATACCAGGTGTAACTTCTCCAGTATCTGTACTGAATTATGCAGGAATTCCTATAACCCATAGAG
GAATATCAAGAGGGTTTCATATTTTTACAGCTATGACTAAGGATAAATTAGATATAGATTGGAAGTCTGTAGTTAATATA
GGAGGAACCTTAGTATTTTTAATGGGGCTTGGAAGATTAGAATTAATAACTAAAGGTCTTATAGAAAATGGAATGGACAA
AGAAGGTAAAGTAGCAGTGGTTATGAAAGGAACTACCTCAAAGCAAAAAAAAGTTATAGGTAACTTAGAAAATATAGTAG
AAAAAGTAAAAGAAGCTAAACTAGAGTCACCAGTAATAATAGTAGTAGGAGAAGTAGTTAGTTTTTCAGATAAATTAAAT
TGGTATGAAAAAAAGCCTTTATTCGGAAGAAATATATGCATAACCCGTACAAAAGAGCAAGCTAAGGAATTAAAAGTTCA
ACTTTTAGATTTAGGGGCTGAAGTTACAGAAATAAATTCTATAGAAATAAAAAATACAGAAGATAATTTAAAGTCTTATT
TAAGTAAATTAAAGGAATATGATTATATAGCTTTAACCAGTGTAAATGCAGTTAAAATATTTTTTGATTATTTAATTAAA
GAAAATATAGATATTAGAAATATTAATGCTAAATTTGCAGCTATAGGACCAGCTACTAGTGAGGCAATAAGGATAAGAGG
AATAATGCCAAGCATTAAGTCAAAGCATTTTGTAGCAGAGAGTCTTTTTGAAGAAATGAGAAAGCATATACAAAAGGGAG
ATAAAATACTTGTGCCTCGTTCTAAAGATGCAAGACCATTTCTTGTGGATGCATTAAGAAAAGAAGGTTGTACTGTGGAT
GAAGTACATATATATGGAACCCTATGTGGACAATGTACATATACTGAAAGATTTGAAGATGCAGATACAGTATTATTTAC
AAGTCCATCTACTGTTAGAAATATGATTTCTATGGTGGGCATAGATAGTATAAAAGAAAAAAGTATTATAGCAATAGGTC
CTATTACAGCAGCAGAATTAGATAAAAATAATATAGAATGTAGTATTTGTGATGAATATTCTATAAATGGCATAATAGAT
AAACTTTTAGACCTTAACTAG

Upstream 100 bases:

>100_bases
CTCATCTTAATAGAAGATGTGAGTATTATAAGTGGTATTAGTGGAAAATAGTAATTTTGAATAAAAAGGTATCCCACACT
TTTATGTTTAGGAGGAAAGT

Downstream 100 bases:

>100_bases
ATGCTGAGAATAATGGTAAATTAATCAACCATATTATAAAAATTAAATCCATCCATTAGGGATAAGAAAGCATATGGGTA
TAGATAAAGAGTACTATGTT

Product: uroporphyrinogen III synthase/methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGCHYKSQDEINKMLVKLAKEGH
IVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPVSVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNI
GGTLVFLMGLGRLELITKGLIENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNTEDNLKSYLSKLKEYDYIALTSVNAVKIFFDYLIK
ENIDIRNINAKFAAIGPATSEAIRIRGIMPSIKSKHFVAESLFEEMRKHIQKGDKILVPRSKDARPFLVDALRKEGCTVD
EVHIYGTLCGQCTYTERFEDADTVLFTSPSTVRNMISMVGIDSIKEKSIIAIGPITAAELDKNNIECSICDEYSINGIID
KLLDLN

Sequences:

>Translated_486_residues
MSKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGCHYKSQDEINKMLVKLAKEGH
IVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPVSVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNI
GGTLVFLMGLGRLELITKGLIENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNTEDNLKSYLSKLKEYDYIALTSVNAVKIFFDYLIK
ENIDIRNINAKFAAIGPATSEAIRIRGIMPSIKSKHFVAESLFEEMRKHIQKGDKILVPRSKDARPFLVDALRKEGCTVD
EVHIYGTLCGQCTYTERFEDADTVLFTSPSTVRNMISMVGIDSIKEKSIIAIGPITAAELDKNNIECSICDEYSINGIID
KLLDLN
>Mature_485_residues
SKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGCHYKSQDEINKMLVKLAKEGHI
VGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPVSVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNIG
GTLVFLMGLGRLELITKGLIENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLNW
YEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNTEDNLKSYLSKLKEYDYIALTSVNAVKIFFDYLIKE
NIDIRNINAKFAAIGPATSEAIRIRGIMPSIKSKHFVAESLFEEMRKHIQKGDKILVPRSKDARPFLVDALRKEGCTVDE
VHIYGTLCGQCTYTERFEDADTVLFTSPSTVRNMISMVGIDSIKEKSIIAIGPITAAELDKNNIECSICDEYSINGIIDK
LLDLN

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=240, Percent_Identity=38.75, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=31.4893617021277, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54055; Mature: 53923

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGC
CCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCC
HYKSQDEINKMLVKLAKEGHIVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPV
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCEEEECCCCCHH
SVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNIGGTLVFLMGLGRLELITKGL
HHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHH
IENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCC
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNTEDNLKSYLSKLKEY
CHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHHHHHHHHC
DYIALTSVNAVKIFFDYLIKENIDIRNINAKFAAIGPATSEAIRIRGIMPSIKSKHFVAE
CEEEEECCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHHH
SLFEEMRKHIQKGDKILVPRSKDARPFLVDALRKEGCTVDEVHIYGTLCGQCTYTERFED
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCC
ADTVLFTSPSTVRNMISMVGIDSIKEKSIIAIGPITAAELDKNNIECSICDEYSINGIID
CCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHCCCCCCEEEEECCCCHHHHHH
KLLDLN
HHHCCC
>Mature Secondary Structure 
SKVYLIGAGPGDEELITLKAIRALKKCTAVMYDRLANGELLKYLAPNCEIYYCGKEPGC
CEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCC
HYKSQDEINKMLVKLAKEGHIVGRIKGGDPYVFGRGGEEALDILEENIEFEVIPGVTSPV
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCEEEECCCCCHH
SVLNYAGIPITHRGISRGFHIFTAMTKDKLDIDWKSVVNIGGTLVFLMGLGRLELITKGL
HHHHCCCCCEEECCCCCCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHH
IENGMDKEGKVAVVMKGTTSKQKKVIGNLENIVEKVKEAKLESPVIIVVGEVVSFSDKLN
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCC
WYEKKPLFGRNICITRTKEQAKELKVQLLDLGAEVTEINSIEIKNTEDNLKSYLSKLKEY
CHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEECCEEEEECCHHHHHHHHHHHHHC
DYIALTSVNAVKIFFDYLIKENIDIRNINAKFAAIGPATSEAIRIRGIMPSIKSKHFVAE
CEEEEECCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHHH
SLFEEMRKHIQKGDKILVPRSKDARPFLVDALRKEGCTVDEVHIYGTLCGQCTYTERFED
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCC
ADTVLFTSPSTVRNMISMVGIDSIKEKSIIAIGPITAAELDKNNIECSICDEYSINGIID
CCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHCCCCCCEEEEECCCCHHHHHH
KLLDLN
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]