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The map label for this gene is rfbB [H]

Identifier: 153935869

GI number: 153935869

Start: 2125419

End: 2126399

Strand: Direct

Name: rfbB [H]

Synonym: CLC_2000

Alternate gene names: 153935869

Gene position: 2125419-2126399 (Clockwise)

Preceding gene: 153935912

Following gene: 153936845

Centisome position: 56.52

GC content: 36.7

Gene sequence:

>981_bases
ATGAGAGTTTTACTTGTAACAGGTGGTGCAGGCTTTATTGGCAGCAATTTTATACGATACTTTCTGGAAACAAACAAAGA
CTTTATTGTAGTTAATTATGATAAGCTTACCTATGCTGGAAACCTTAATAATTTAAAGGAAGTTGAAATGCTTCCGACTT
ATCGCTTTGTTAAGGGCGATATATGTGACAGCGAGGAATTCAATAGGACATTGAAGAAGTATAACCCTGACTATATAATC
AATTTTGCTGCTGAATCGCATGTAGATAGAAGTATAAATGGACCTTCAGTATTTGGTCAAACGAACTTTATGGGAACACT
TAATTTACTCCAGTGTGCTCATGAATTTTGGGGTAGCAGCTGTGCGGACAAGCGTTTTTTGCAGGTCTCAACGGATGAAG
TATATGGAAGTATTGAGAATGACAGCGATTATTTTATAGAAGATTCAAATCTCATGCCTAACAGTCCTTATTCAGCCTCA
AAGGCAGGAGCAGATATGATGGTAAGAGCCTTTGGGAGAACTTACGGACTTCCTGTTATAATTACAAGATGCTGTAATAA
TTATGGACCGTACCAATACAGTGAGAAGCTTATTCCTATCTGTATTATAAAAGCTTTAAATGATGAGCCTATACCAATAT
ATGGTGATGGCACAAATATAAGAGAATGGATTCATGTATCAGATCACTGTTCTGCAATTATAAAAGCGTTATTTTATGGT
ACGCCAGGAGAAGTTTACAATATTGGAAGTGGAGAAGAGGTTTCAAATGTTGATATGGCAAAGATAATTCTTAGTAATCT
TAGCAAACCAACGGATGCCATAAAAAAAGTGAATGACAGGTTGGGCCATGACAGACGGTATGCGTTGGATAGCAGTAAAA
TAAAAAACCAGTTAAGCTGGGCTTGCAGCTATAAGCTGGAAGAAGGAATCAAGGAAACAATTGAATGGTATAAAAATAAC
CAGACCTGGTGGAATGATTAA

Upstream 100 bases:

>100_bases
ATAAGAGACCGTTTTCTCATACTTTCCTTTTAGGTGATGATAGTGAAATTGATCTTTAGAGAAAATTAAATATAAATTCA
TGATTGGAGGAAGTTTTTAA

Downstream 100 bases:

>100_bases
TATTTAAATTAGCAAGTAAGAAATCAATTCATTAATTGCTACTTGACCTTAGAGTATTCAAAATTTAAAGAATTTTATTA
TTTTCATTGCTTAGAGTATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGDICDSEEFNRTLKKYNPDYII
NFAAESHVDRSINGPSVFGQTNFMGTLNLLQCAHEFWGSSCADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSAS
KAGADMMVRAFGRTYGLPVIITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSWACSYKLEEGIKETIEWYKNN
QTWWND

Sequences:

>Translated_326_residues
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGDICDSEEFNRTLKKYNPDYII
NFAAESHVDRSINGPSVFGQTNFMGTLNLLQCAHEFWGSSCADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSAS
KAGADMMVRAFGRTYGLPVIITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSWACSYKLEEGIKETIEWYKNN
QTWWND
>Mature_326_residues
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGDICDSEEFNRTLKKYNPDYII
NFAAESHVDRSINGPSVFGQTNFMGTLNLLQCAHEFWGSSCADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSAS
KAGADMMVRAFGRTYGLPVIITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSWACSYKLEEGIKETIEWYKNN
QTWWND

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=38.4384384384384, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI42516563, Length=320, Percent_Identity=25.625, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=46.7647058823529, Blast_Score=321, Evalue=3e-89,
Organism=Escherichia coli, GI1788353, Length=339, Percent_Identity=45.4277286135693, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=26.1971830985915, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788366, Length=357, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=38.1987577639752, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI115532424, Length=321, Percent_Identity=31.1526479750779, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17539532, Length=328, Percent_Identity=25.9146341463415, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=26.0623229461756, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=25.3822629969419, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37004; Mature: 37004

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGD
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCHHHHHCCCCCEEECC
ICDSEEFNRTLKKYNPDYIINFAAESHVDRSINGPSVFGQTNFMGTLNLLQCAHEFWGSS
CCCCHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCC
CADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSASKAGADMMVRAFGRTYGLPVI
CCCCCEEEEEHHHHEECCCCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCHHHH
ITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
HHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHEEHHHHHHHHHHHHHCC
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSW
CCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEECCHHHHHHHHHH
ACSYKLEEGIKETIEWYKNNQTWWND
HHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGD
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCHHHHHCCCCCEEECC
ICDSEEFNRTLKKYNPDYIINFAAESHVDRSINGPSVFGQTNFMGTLNLLQCAHEFWGSS
CCCCHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCC
CADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSASKAGADMMVRAFGRTYGLPVI
CCCCCEEEEEHHHHEECCCCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCHHHH
ITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
HHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHEEHHHHHHHHHHHHHCC
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSW
CCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEECCHHHHHHHHHH
ACSYKLEEGIKETIEWYKNNQTWWND
HHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]