| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
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The map label for this gene is helD [H]
Identifier: 153935697
GI number: 153935697
Start: 793138
End: 795396
Strand: Direct
Name: helD [H]
Synonym: CLC_0766
Alternate gene names: 153935697
Gene position: 793138-795396 (Clockwise)
Preceding gene: 153936378
Following gene: 153937115
Centisome position: 21.09
GC content: 33.73
Gene sequence:
>2259_bases ATGAAGGATTTTGAATGGAAGCTTGAAAAGGAATGGTTGCAAGAGGTGTTTCAAGAGGTTCGAAAACAGTATAATGAAAA ACATGATCTTAAAGAAAAGTTTAAAAAGGATGCCATTGAAACACAACGGGAACTATGGAATGATGTAGGCTCTGTTTCCA TAGCCAATGGATTAGACCAGGTTGTAGACTTTATAGGATTTATCAATACCATGAAAATACAGAAGAGAAGCCACGAGTTC ACGAAAAAGCTTGAGGAAAAATACGAAAGAATGCTCCTATCACCCTACTTTGGAAGGATGGACTTTATTGAAAATGGAGA GGAGAAAGTTGAAAAGTATTACATTGGAATCTCAAATCTTATTAATGATAACTATGATTTTCTCGTATACGACTGGAGAG CACCGGTTTCCAGCATGTTTTATGATTACGAAATTGGAGAAGCCAATTATAAATGTCCTGAAGGAAGGGTAAATGGGAAA CTTACACTGAAAAGACAGTACAAAATTAATAATGGTGAAATTAAGTATATGTTTGACAGCAATCTTAAGATAGACGATGA AGTGCTACAGGATATCTTGGGTAAAAGCACGGATAGTAAGATGAATGCAATAGTAACAACTATTCAAAGAGAGCAAAATA AAGTAATTCGTAATGAGGATTATAAAAATTTGATTGTGCAAGGTCCTGCTGGAAGTGGGAAAACCTCTGTTGCTCTTCAT AGAATTGCCTATCTATTATATAAGCATAGGGATAAAATAAGCCCTAAAAATATAGTAATATTCTCTCCAAACGATATTTT AAATGATTATATTTCTAATGTATTGCCCCAGCTTGGAGAAGATAATATGTGTCAGACCACATTTAAGGAATACATGCACA AGGCTTTAGGCAATAGGCTTATAAAGGAAAACTATTCTGAGATGATGGAGTATATTCTTGTCTCTAAGAAGGATGGTGCC TATGAAAAGAGAATAAACAATATAAAATTTAAGTCTTCCATGGAGTTTATGGATATATTAAAAGCATATGTAGGCTACCT AGAAAAAATGGATAGAAATTTTACAGATATTACTTTCAGAGGGGATTTGATAATCTCATCTAAGGATATAGAAGAATTAT TTTTTGAAGATTATGTAAAGCTTCCAATTAAAAGAAGGCTTCAAAAGATAAGAAGAAGAATTTTGTTCCTAATGGAGCCT TATGAAAAACATTGGGTAAAAGAAGAGGCTGAGGAGCTTAAGAATTCAGGTAACTATATAGATAATGTAGAAATTACGGA GCGCAGTATAAATATTGTAAAGGATGAAATAAAGGATATATACCATGAAATCAATAGAATGACAGACTTTGATTTAGTGG ATGTTTATAAAAAGCTTTTTGAAAACCTGGAGTTTTTCTTAAAAAAATCAAATACTGAATACCATGAGAAAAATATTGAT GAAATGAAAAGCTATACTTTAGAAGCCTTAAGAATAGGAAAACTTAACTATGAGGATCAGCCACCCCTTTTGTATCTAAA AGGTGCCTTAGGAGACCTGCCAAAAAACTCAGAAATCAAATATGTTATTATTGATGAGGCTCAGGATTATACACCGCTAC AGTATGAAATCTTTTCTCAGCTTTTCAACCATGCCAATATGACTATACTAGGGGATTTACATCAATCCATTAATCCCTTC ATGAACGTGGGGGATTATAGTAATATCTCCAATGTATTCCCAAAGGATAATACATGCATAATAAATTTAACCAAGAGTTA CAGATCCACAATGGAAATTACTGAGTTTTCAAGAAGGATACTTAACAAGAAGATTACTGACCAATGGGTAGAAAGAAGTG GAGACAAGCCTTTAGTCCTTGGCTTTGCAGATGAAGAGGCTATAAAGGAAAGACTTCTAAAGGATATAAAAATATATAAG GAGAAAGGCTACAAATCTATTGGCATCATAACAAGGACTATAAAGGAAGCGCAAGAGGTACACAGTTTCTTAAAAGATAA GATTCATGTTAAAGCCATAATGAAGGATGATGATGAATATGTGAGTGATACCTTGGTAATCCCCGCTTATCTTGCTAAAG GATTAGAATTTGATGTGGTGTTAATATATAATGCAGGGGATGGAAATTACAGCTGCCAAGAGGAAAGGCTGCTGCTTTAT ACTGCGTGTACTAGGGCACTTCATCTTTTATGCATATACTATTCAGGAAAGGTTACCCCCTTGTTAGTAGGAAGTATTGA ATTTAGCACTTTAAAATAG
Upstream 100 bases:
>100_bases CTAACTGTTAAAATATAATAATCAAATATAGAACTATACTTCATGTAAAAAAGACATTTGTCATTGGGCGAATGTCTTTA CTATGTAAGGAGGGGATATA
Downstream 100 bases:
>100_bases AAGACAATTTTGATTGTTAAAAAGCTGTAGATAAAACTACAGCTTTTTTAGTGCACAAAAGGCTAGTACATAATTTGGAT CAAAAAGTTTATATCTCACT
Product: putative helicase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 752; Mature: 752
Protein sequence:
>752_residues MKDFEWKLEKEWLQEVFQEVRKQYNEKHDLKEKFKKDAIETQRELWNDVGSVSIANGLDQVVDFIGFINTMKIQKRSHEF TKKLEEKYERMLLSPYFGRMDFIENGEEKVEKYYIGISNLINDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGK LTLKRQYKINNGEIKYMFDSNLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEDYKNLIVQGPAGSGKTSVALH RIAYLLYKHRDKISPKNIVIFSPNDILNDYISNVLPQLGEDNMCQTTFKEYMHKALGNRLIKENYSEMMEYILVSKKDGA YEKRINNIKFKSSMEFMDILKAYVGYLEKMDRNFTDITFRGDLIISSKDIEELFFEDYVKLPIKRRLQKIRRRILFLMEP YEKHWVKEEAEELKNSGNYIDNVEITERSINIVKDEIKDIYHEINRMTDFDLVDVYKKLFENLEFFLKKSNTEYHEKNID EMKSYTLEALRIGKLNYEDQPPLLYLKGALGDLPKNSEIKYVIIDEAQDYTPLQYEIFSQLFNHANMTILGDLHQSINPF MNVGDYSNISNVFPKDNTCIINLTKSYRSTMEITEFSRRILNKKITDQWVERSGDKPLVLGFADEEAIKERLLKDIKIYK EKGYKSIGIITRTIKEAQEVHSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLAKGLEFDVVLIYNAGDGNYSCQEERLLLY TACTRALHLLCIYYSGKVTPLLVGSIEFSTLK
Sequences:
>Translated_752_residues MKDFEWKLEKEWLQEVFQEVRKQYNEKHDLKEKFKKDAIETQRELWNDVGSVSIANGLDQVVDFIGFINTMKIQKRSHEF TKKLEEKYERMLLSPYFGRMDFIENGEEKVEKYYIGISNLINDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGK LTLKRQYKINNGEIKYMFDSNLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEDYKNLIVQGPAGSGKTSVALH RIAYLLYKHRDKISPKNIVIFSPNDILNDYISNVLPQLGEDNMCQTTFKEYMHKALGNRLIKENYSEMMEYILVSKKDGA YEKRINNIKFKSSMEFMDILKAYVGYLEKMDRNFTDITFRGDLIISSKDIEELFFEDYVKLPIKRRLQKIRRRILFLMEP YEKHWVKEEAEELKNSGNYIDNVEITERSINIVKDEIKDIYHEINRMTDFDLVDVYKKLFENLEFFLKKSNTEYHEKNID EMKSYTLEALRIGKLNYEDQPPLLYLKGALGDLPKNSEIKYVIIDEAQDYTPLQYEIFSQLFNHANMTILGDLHQSINPF MNVGDYSNISNVFPKDNTCIINLTKSYRSTMEITEFSRRILNKKITDQWVERSGDKPLVLGFADEEAIKERLLKDIKIYK EKGYKSIGIITRTIKEAQEVHSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLAKGLEFDVVLIYNAGDGNYSCQEERLLLY TACTRALHLLCIYYSGKVTPLLVGSIEFSTLK >Mature_752_residues MKDFEWKLEKEWLQEVFQEVRKQYNEKHDLKEKFKKDAIETQRELWNDVGSVSIANGLDQVVDFIGFINTMKIQKRSHEF TKKLEEKYERMLLSPYFGRMDFIENGEEKVEKYYIGISNLINDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGK LTLKRQYKINNGEIKYMFDSNLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEDYKNLIVQGPAGSGKTSVALH RIAYLLYKHRDKISPKNIVIFSPNDILNDYISNVLPQLGEDNMCQTTFKEYMHKALGNRLIKENYSEMMEYILVSKKDGA YEKRINNIKFKSSMEFMDILKAYVGYLEKMDRNFTDITFRGDLIISSKDIEELFFEDYVKLPIKRRLQKIRRRILFLMEP YEKHWVKEEAEELKNSGNYIDNVEITERSINIVKDEIKDIYHEINRMTDFDLVDVYKKLFENLEFFLKKSNTEYHEKNID EMKSYTLEALRIGKLNYEDQPPLLYLKGALGDLPKNSEIKYVIIDEAQDYTPLQYEIFSQLFNHANMTILGDLHQSINPF MNVGDYSNISNVFPKDNTCIINLTKSYRSTMEITEFSRRILNKKITDQWVERSGDKPLVLGFADEEAIKERLLKDIKIYK EKGYKSIGIITRTIKEAQEVHSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLAKGLEFDVVLIYNAGDGNYSCQEERLLLY TACTRALHLLCIYYSGKVTPLLVGSIEFSTLK
Specific function: Catalyzes the unwinding of duplex DNA in the 3' to 5' direction; this reaction is dependent on the hydrolysis of ATP [H]
COG id: COG3973
COG function: function code R; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000212 - InterPro: IPR014016 [H]
Pfam domain/function: PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 88471; Mature: 88471
Theoretical pI: Translated: 5.90; Mature: 5.90
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKDFEWKLEKEWLQEVFQEVRKQYNEKHDLKEKFKKDAIETQRELWNDVGSVSIANGLDQ CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH VVDFIGFINTMKIQKRSHEFTKKLEEKYERMLLSPYFGRMDFIENGEEKVEKYYIGISNL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH INDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGKLTLKRQYKINNGEIKYMFDS HCCCCCEEEEECCCCHHHHHEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECC NLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEDYKNLIVQGPAGSGKTSVALH CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH RIAYLLYKHRDKISPKNIVIFSPNDILNDYISNVLPQLGEDNMCQTTFKEYMHKALGNRL HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH IKENYSEMMEYILVSKKDGAYEKRINNIKFKSSMEFMDILKAYVGYLEKMDRNFTDITFR HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE GDLIISSKDIEELFFEDYVKLPIKRRLQKIRRRILFLMEPYEKHWVKEEAEELKNSGNYI CCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEE DNVEITERSINIVKDEIKDIYHEINRMTDFDLVDVYKKLFENLEFFLKKSNTEYHEKNID CCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHH EMKSYTLEALRIGKLNYEDQPPLLYLKGALGDLPKNSEIKYVIIDEAQDYTPLQYEIFSQ HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH LFNHANMTILGDLHQSINPFMNVGDYSNISNVFPKDNTCIINLTKSYRSTMEITEFSRRI HHCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH LNKKITDQWVERSGDKPLVLGFADEEAIKERLLKDIKIYKEKGYKSIGIITRTIKEAQEV HHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH HSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLAKGLEFDVVLIYNAGDGNYSCQEERLLLY HHHHHHHHEEEEEECCCHHHHHHHEEHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHH TACTRALHLLCIYYSGKVTPLLVGSIEFSTLK HHHHHHHHHHHHEECCCCCEEEEECEECCCCC >Mature Secondary Structure MKDFEWKLEKEWLQEVFQEVRKQYNEKHDLKEKFKKDAIETQRELWNDVGSVSIANGLDQ CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH VVDFIGFINTMKIQKRSHEFTKKLEEKYERMLLSPYFGRMDFIENGEEKVEKYYIGISNL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH INDNYDFLVYDWRAPVSSMFYDYEIGEANYKCPEGRVNGKLTLKRQYKINNGEIKYMFDS HCCCCCEEEEECCCCHHHHHEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECC NLKIDDEVLQDILGKSTDSKMNAIVTTIQREQNKVIRNEDYKNLIVQGPAGSGKTSVALH CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH RIAYLLYKHRDKISPKNIVIFSPNDILNDYISNVLPQLGEDNMCQTTFKEYMHKALGNRL HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH IKENYSEMMEYILVSKKDGAYEKRINNIKFKSSMEFMDILKAYVGYLEKMDRNFTDITFR HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE GDLIISSKDIEELFFEDYVKLPIKRRLQKIRRRILFLMEPYEKHWVKEEAEELKNSGNYI CCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEE DNVEITERSINIVKDEIKDIYHEINRMTDFDLVDVYKKLFENLEFFLKKSNTEYHEKNID CCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHH EMKSYTLEALRIGKLNYEDQPPLLYLKGALGDLPKNSEIKYVIIDEAQDYTPLQYEIFSQ HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH LFNHANMTILGDLHQSINPFMNVGDYSNISNVFPKDNTCIINLTKSYRSTMEITEFSRRI HHCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH LNKKITDQWVERSGDKPLVLGFADEEAIKERLLKDIKIYKEKGYKSIGIITRTIKEAQEV HHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH HSFLKDKIHVKAIMKDDDEYVSDTLVIPAYLAKGLEFDVVLIYNAGDGNYSCQEERLLLY HHHHHHHHEEEEEECCCHHHHHHHEEHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHH TACTRALHLLCIYYSGKVTPLLVGSIEFSTLK HHHHHHHHHHHHEECCCCCEEEEECEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]