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The map label for this gene is mscL [H]

Identifier: 153934918

GI number: 153934918

Start: 627675

End: 628076

Strand: Direct

Name: mscL [H]

Synonym: CLC_0611

Alternate gene names: 153934918

Gene position: 627675-628076 (Clockwise)

Preceding gene: 153937323

Following gene: 153936119

Centisome position: 16.69

GC content: 26.62

Gene sequence:

>402_bases
TTGAAAAAGATAATTGAAGAATTCAAAGATTTTGCAGTAAAGGGTAATGCTATAGAATTAGCAGTAGGTGTAGTTATAGG
AAGTGCTTTTGGGAAAATAGTAACTTCCTTAGTAGAGGATATTATAATGCCTTTGGTAGGATTGCTAATAGGTGGTATAG
ATTTTACTGGGTTAAAATTTTCAATGAATCTTTCAGGAAAAACAGTAGTTTCTATAAATTACGGTAATTTTATACAGGCA
GCAGTAAATTTTTTAATAATATCTTTTTCTATTTTCTTGTTTATTAAATTAATAAATAAATTTAAGCAAAATGAAGAAGA
AGAAAAAGACGAACCTAAAATAAGCAATGAAGAGATATTACTAAGAGAAATAAGGGATTTGTTAAAGGAAAATAAATCCT
AA

Upstream 100 bases:

>100_bases
AATATATCATCTATATTTTACTAGGCCAAAATTCTTGAAAATAAACTAGGTTTATTATATTATACATAATAGCTTATAGT
TCAAGATGGAGGTTTTAATT

Downstream 100 bases:

>100_bases
TAGTTTGCTTATTATTTTTAAAGAGAATTGATACAATTTTAGGAATATTAAATTTAGACTATTAAAATTATAGAGATAAA
TGAATTATTTTTATTATTGA

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKKIIEEFKDFAVKGNAIELAVGVVIGSAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLKFSMNLSGKTVVSINYGNFIQA
AVNFLIISFSIFLFIKLINKFKQNEEEEKDEPKISNEEILLREIRDLLKENKS

Sequences:

>Translated_133_residues
MKKIIEEFKDFAVKGNAIELAVGVVIGSAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLKFSMNLSGKTVVSINYGNFIQA
AVNFLIISFSIFLFIKLINKFKQNEEEEKDEPKISNEEILLREIRDLLKENKS
>Mature_133_residues
MKKIIEEFKDFAVKGNAIELAVGVVIGSAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLKFSMNLSGKTVVSINYGNFIQA
AVNFLIISFSIFLFIKLINKFKQNEEEEKDEPKISNEEILLREIRDLLKENKS

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=132, Percent_Identity=55.3030303030303, Blast_Score=139, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14842; Mature: 14842

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIEEFKDFAVKGNAIELAVGVVIGSAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLKF
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
SMNLSGKTVVSINYGNFIQAAVNFLIISFSIFLFIKLINKFKQNEEEEKDEPKISNEEIL
EECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHH
LREIRDLLKENKS
HHHHHHHHHCCCC
>Mature Secondary Structure
MKKIIEEFKDFAVKGNAIELAVGVVIGSAFGKIVTSLVEDIIMPLVGLLIGGIDFTGLKF
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
SMNLSGKTVVSINYGNFIQAAVNFLIISFSIFLFIKLINKFKQNEEEEKDEPKISNEEIL
EECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHH
LREIRDLLKENKS
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9922257 [H]