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The map label for this gene is vanS [H]

Identifier: 153934845

GI number: 153934845

Start: 417820

End: 419334

Strand: Direct

Name: vanS [H]

Synonym: CLC_0424

Alternate gene names: 153934845

Gene position: 417820-419334 (Clockwise)

Preceding gene: 153936832

Following gene: 153935356

Centisome position: 11.11

GC content: 23.89

Gene sequence:

>1515_bases
TTGAAAAATAGAATAAAGAGATTATTTAATAAAATAATGAAACCTTTCAAATATATATATAAATTGATATCTTATAAAGT
AAAAAAAAGTTTAAGATTAGAACTTATATTTACTTTTGGATTATGTTTTTTAATGGCTATTATGACCTTTATTATAACTA
ATAGTTACCTAACTAAAGCTAGTAAGTATTCAGAAATAAATTATGAAAAAGGAATAAATGAAATTTACAATTATTCTTTA
TCTATATCAAATGAAATTAATAGTGAAAAATTAAAAATAGAAGATAAAGACACTATACAAGAACTTATAGATAGACATTG
GAGCATGGATAAGAATAATAAAATATTTATAGCTAATACTGATGGCAAAATTCTTTTCAAAGTAGAAAATGTAGAAGTTG
AAAAAGTTGATATATTTGAAATTATAAAAAATAATATACAACTTCAAAATATAAAGAGTTATGAGTATGATTATGATGTA
GGAAGAAAAGAATTTGTAAGTATCAATCCATTAATTTTTGAAAATGGAAAAGCTTATGTAATAGTTCAAGGCATACCAAG
AGCAGAAACCATATATTATACAAAGGATAAATCTGTGAGTGCATTTTTATTAGCTGTTATAGTATTTATATTTGGATTTT
TATTTATAACAAAAAGGAAGATGAATTATATAGAAGAAATATCAAAGGGCTTGCTGCAAATATCAAAAGGAAATTTAGAT
TATAGAGTAAAAAGAGTAGGAAATGATGAGTTAGCACTACTAGCAGATAATATAAATTATATGGCAAAAGAGTTAACTGA
TAAAATAGAAAAAGAAAGAAAAATAGAAAAAGCTAAAAATGACTTAATAACAAATGTCTCCCATGATTTAAGAACTCCAC
TTACATCTATAATGGGGTATTTAGGATTAATAAAAGAAAAAAAATTTAAAAGTGAAAAAGAATTAATGGAATATGCAAGT
GTAGCTTACACTAAATCGGAAAAACTAAAAAATTTAATACAGGATTTATTTTCTTATACTAAATATAGTAATGACAGAGT
AGAACTAAACAAGCAGAAGATCATTTTAGCAGAATTATTAGATCAATTAATAGAAGAATTAGTACCTATATGTGAAGAAA
ATAAAGTAAGTATAAATAAATATGCTTGGGATTCTGAAATAGTATCAGAAATAGATGCAGACAAAATGGTAAGAGTTTTT
GAAAATCTAATAATGAATGCTATAAGATATAGTATAAAGCCAGGAGAAATAAAAATTAATTTGTACAAAGAAAGAGTATT
TTCTATGGTATCCATATCAAATAAAAGTGAAGAAATATCAAAAGATAACTTAAGAAAATTATTTGATAGATTTTATAGAT
TAGATAAATCAAGAACTGCATCAACCGGTGGAAGTGGTTTAGGATTAGCTATAGCCAAGAGTATAGTAGAATTGCATAAA
GGTAGCATTTGGGCGGAATATCAAAAGGGATATATAACTTTTTATATAAAATTAATTATGGCAGAGGATGAATAA

Upstream 100 bases:

>100_bases
AATACAGTAATGGTTCATATAAGAAAATTAAGAGAAAAAATAGAACAAAATCCTAGAAATCCAAGATATATAAAAACAGT
TTGGGGAGTTGGGTATAAGG

Downstream 100 bases:

>100_bases
AATTTATTAAAACCTGGTGCAATTGCATCAGGTTTTAAGTTATAATATAATGAAAAATATTATATATAAAATATTTATTA
AACAAACATATTTAAGAGGA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKNRIKRLFNKIMKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGINEIYNYSL
SISNEINSEKLKIEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDV
GRKEFVSINPLIFENGKAYVIVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLD
YRVKRVGNDELALLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSEKELMEYAS
VAYTKSEKLKNLIQDLFSYTKYSNDRVELNKQKIILAELLDQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVF
ENLIMNAIRYSIKPGEIKINLYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHK
GSIWAEYQKGYITFYIKLIMAEDE

Sequences:

>Translated_504_residues
MKNRIKRLFNKIMKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGINEIYNYSL
SISNEINSEKLKIEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDV
GRKEFVSINPLIFENGKAYVIVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLD
YRVKRVGNDELALLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSEKELMEYAS
VAYTKSEKLKNLIQDLFSYTKYSNDRVELNKQKIILAELLDQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVF
ENLIMNAIRYSIKPGEIKINLYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHK
GSIWAEYQKGYITFYIKLIMAEDE
>Mature_504_residues
MKNRIKRLFNKIMKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGINEIYNYSL
SISNEINSEKLKIEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDV
GRKEFVSINPLIFENGKAYVIVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLD
YRVKRVGNDELALLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSEKELMEYAS
VAYTKSEKLKNLIQDLFSYTKYSNDRVELNKQKIILAELLDQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVF
ENLIMNAIRYSIKPGEIKINLYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHK
GSIWAEYQKGYITFYIKLIMAEDE

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=285, Percent_Identity=28.0701754385965, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786783, Length=298, Percent_Identity=25.8389261744966, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1786600, Length=249, Percent_Identity=25.3012048192771, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790346, Length=259, Percent_Identity=25.8687258687259, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787894, Length=325, Percent_Identity=24.6153846153846, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788713, Length=243, Percent_Identity=24.2798353909465, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788549, Length=251, Percent_Identity=27.4900398406374, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=25.5905511811024, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=24.7787610619469, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788279, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI145693157, Length=278, Percent_Identity=21.2230215827338, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=289, Percent_Identity=25.2595155709343, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=249, Percent_Identity=22.4899598393574, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082128, Length=327, Percent_Identity=23.5474006116208, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=335, Percent_Identity=23.5820895522388, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787374, Length=402, Percent_Identity=23.6318407960199, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58850; Mature: 58850

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRIKRLFNKIMKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKYSEINYEKGINEIYNYSLSISNEINSEKLKIEDKDTIQELIDRHWSMDKNNKIFIANT
HHHCCCCHHHCHHHHHCCEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCCCEEEEEEC
DGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDVGRKEFVSINPLIFENGKAYV
CCEEEEEEECCEEEHHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEEECCEEEECCCEEE
IVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLD
EEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
YRVKRVGNDELALLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGY
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGLIKEKKFKSEKELMEYASVAYTKSEKLKNLIQDLFSYTKYSNDRVELNKQKIILAELL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHH
DQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVFENLIMNAIRYSIKPGEIKIN
HHHHHHHHCCCCCCCCEEHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHK
EHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
GSIWAEYQKGYITFYIKLIMAEDE
CCEEEHHHCCEEEEEEEEEEECCC
>Mature Secondary Structure
MKNRIKRLFNKIMKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKYSEINYEKGINEIYNYSLSISNEINSEKLKIEDKDTIQELIDRHWSMDKNNKIFIANT
HHHCCCCHHHCHHHHHCCEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCCCEEEEEEC
DGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDVGRKEFVSINPLIFENGKAYV
CCEEEEEEECCEEEHHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEEECCEEEECCCEEE
IVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLD
EEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
YRVKRVGNDELALLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGY
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LGLIKEKKFKSEKELMEYASVAYTKSEKLKNLIQDLFSYTKYSNDRVELNKQKIILAELL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHH
DQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVFENLIMNAIRYSIKPGEIKIN
HHHHHHHHCCCCCCCCEEHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHK
EHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
GSIWAEYQKGYITFYIKLIMAEDE
CCEEEHHHCCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]