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The map label for this gene is vanS [H]

Identifier: 153934812

GI number: 153934812

Start: 683014

End: 684135

Strand: Direct

Name: vanS [H]

Synonym: CLC_0666

Alternate gene names: 153934812

Gene position: 683014-684135 (Clockwise)

Preceding gene: 153935657

Following gene: 153936715

Centisome position: 18.16

GC content: 25.85

Gene sequence:

>1122_bases
TTGAAAAATAAAAGATTATATAAATATTGTTTATCGTCGTTGAGAGACATATTAATAGCTTTAATATTAACTTACACAAC
AGCGTATATAAGTAAGAAAATATTAAACTATTTATATGTAAAGTTTGACAATGAGTTTGCTTTTTATTTCTGGACGCTTT
GGAGAAATGTTAAATTTGCAACAGTTGGTCAATCTCTTAATATAATAGTTGGAGTAGTTTTATTTTCAATGTTCTACTTG
ATAATAACTTATAGAAAAGCTAAAAATTTTGTAGCTATTATAGAGGAAACTGAAATAATGGCTAATGGTGATTTAGATAG
AGTTATACAGGTTAATGCAGAAGGGGATATTAAGAATTTAGCAGAAAATATAAATAATATATCAAAGCAACTTAAAGATA
TAACAGTAGCAGAAAGAAACGCACAGAAAACCAAAAATGATTTGATAACTAATGTCTCCCACGATTTAAGAACTCCATTG
ACTTCAATAATGGGTTATTTAGAACTTATTGATAATGACCAATATAAAGATGAAGTGGCACTTAGATATTATGTTAATAT
AGCATATGAAAAATCTAAGGGGTTAAATGTGCTTATAAAGGATTTGTTTGAACTTACTAAAATGCGAAATAATACTATTA
ATCTAGAGAAAGTTGATATTAATTTAGTGGAATTATTAGGGCAAGTAGTTGCTTATTTTGAATATCAATTTAAAAGTGCA
AATATGAAATCAAGAATTAAATTTTCAAACGATAAGCTAATAGTAAATGCAGATGCAGGAAAACTAGTAAGAGCATTTGA
AAATTTATTATCTAATGCAATTAAATATGGGAAAGACGGTTATTATGTTGATGTAGTAACAAAGCTTGAAGAAAATATGG
CAGTAATTCAAATTATAAATTATGGTCAATTAATACCAACAGTAGACTTACCATATATATTTGATAGATTCTATAGAGTA
GAAAAATCAAGAAATAACAAAATAGGGGGATCAGGATTAGGTTTAGCTATAACGAAAAATATTATAGAATTGCATGAAGG
TACTATAAAAGCATATAGTAATGACAATAAAACTGTATTTGAAATAAAATTACCTATTAAGATTAATATAAATAGCAATT
AA

Upstream 100 bases:

>100_bases
AATACTGTTATGGTACATATGAGTAGACTTAGAGATAAAATAGAACAACATATGGAAGGTAGTAAGGTAATCCATACTGT
GTGGGGAGTTGGTTACAAAA

Downstream 100 bases:

>100_bases
ATAAAAATAATAATATATATTTATATGATACCAATAGATTAATAAATTATAAAGCCCCATGCAAATGCATAGGGTTTTTG
AATAATCTAAGCAAATATAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKNKRLYKYCLSSLRDILIALILTYTTAYISKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVVLFSMFYL
IITYRKAKNFVAIIEETEIMANGDLDRVIQVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPL
TSIMGYLELIDNDQYKDEVALRYYVNIAYEKSKGLNVLIKDLFELTKMRNNTINLEKVDINLVELLGQVVAYFEYQFKSA
NMKSRIKFSNDKLIVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRV
EKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN

Sequences:

>Translated_373_residues
MKNKRLYKYCLSSLRDILIALILTYTTAYISKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVVLFSMFYL
IITYRKAKNFVAIIEETEIMANGDLDRVIQVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPL
TSIMGYLELIDNDQYKDEVALRYYVNIAYEKSKGLNVLIKDLFELTKMRNNTINLEKVDINLVELLGQVVAYFEYQFKSA
NMKSRIKFSNDKLIVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRV
EKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN
>Mature_373_residues
MKNKRLYKYCLSSLRDILIALILTYTTAYISKKILNYLYVKFDNEFAFYFWTLWRNVKFATVGQSLNIIVGVVLFSMFYL
IITYRKAKNFVAIIEETEIMANGDLDRVIQVNAEGDIKNLAENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPL
TSIMGYLELIDNDQYKDEVALRYYVNIAYEKSKGLNVLIKDLFELTKMRNNTINLEKVDINLVELLGQVVAYFEYQFKSA
NMKSRIKFSNDKLIVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIINYGQLIPTVDLPYIFDRFYRV
EKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVFEIKLPIKININSN

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=28.2685512367491, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1786783, Length=295, Percent_Identity=27.7966101694915, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=30.4166666666667, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=24.8, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=25.2066115702479, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789149, Length=275, Percent_Identity=26.9090909090909, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=28.5123966942149, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=232, Percent_Identity=26.7241379310345, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=30.6034482758621, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789808, Length=254, Percent_Identity=25.1968503937008, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788279, Length=312, Percent_Identity=26.2820512820513, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787894, Length=230, Percent_Identity=27.8260869565217, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=28.2700421940928, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=24.8945147679325, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=199, Percent_Identity=25.6281407035176, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=263, Percent_Identity=21.6730038022814, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42964; Mature: 42964

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKRLYKYCLSSLRDILIALILTYTTAYISKKILNYLYVKFDNEFAFYFWTLWRNVKFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEHHHCCEEE
TVGQSLNIIVGVVLFSMFYLIITYRKAKNFVAIIEETEIMANGDLDRVIQVNAEGDIKNL
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEECCCCCHHHH
AENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQYKDEVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHEE
LRYYVNIAYEKSKGLNVLIKDLFELTKMRNNTINLEKVDINLVELLGQVVAYFEYQFKSA
EEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC
NMKSRIKFSNDKLIVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIIN
CCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEEC
YGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVF
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEE
EIKLPIKININSN
EEEEEEEEEECCC
>Mature Secondary Structure
MKNKRLYKYCLSSLRDILIALILTYTTAYISKKILNYLYVKFDNEFAFYFWTLWRNVKFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEHHHCCEEE
TVGQSLNIIVGVVLFSMFYLIITYRKAKNFVAIIEETEIMANGDLDRVIQVNAEGDIKNL
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCEEEECCCCCHHHH
AENINNISKQLKDITVAERNAQKTKNDLITNVSHDLRTPLTSIMGYLELIDNDQYKDEVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHEE
LRYYVNIAYEKSKGLNVLIKDLFELTKMRNNTINLEKVDINLVELLGQVVAYFEYQFKSA
EEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC
NMKSRIKFSNDKLIVNADAGKLVRAFENLLSNAIKYGKDGYYVDVVTKLEENMAVIQIIN
CCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCEEEEEEEC
YGQLIPTVDLPYIFDRFYRVEKSRNNKIGGSGLGLAITKNIIELHEGTIKAYSNDNKTVF
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEE
EIKLPIKININSN
EEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]