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The map label for this gene is fumB [H]

Identifier: 153934695

GI number: 153934695

Start: 3556288

End: 3556842

Strand: Reverse

Name: fumB [H]

Synonym: CLC_3385

Alternate gene names: 153934695

Gene position: 3556842-3556288 (Counterclockwise)

Preceding gene: 153936630

Following gene: 153935656

Centisome position: 94.58

GC content: 31.35

Gene sequence:

>555_bases
ATGAAAATAAAAATAAATACTCCATTAACTGAAGATAAAATCAAAAGCTTAAAAGCAGGTGATATGGTTTTAATTACAGG
TACGATATATACAGCTAGAGATGCAGCACATAAAAGGTTAATTGATGCTTTAGAAAAAGGAAGGAATTTACCATTTGAAG
TGAAAAATTCAATAATATATTATGTTGGACCTACTCCGGCTAAGCCTGGCATGGAAATAGGAGCAGCAGGGCCAACTACA
AGTTATAGGATGGATACATATACACCCAAACTATTGAATTTAGGATTAAAGGGAATGATAGGTAAAGGTAGAAGATCTAA
AGAGGTTATAGAATCTATAGTAAAAAATAAAGCAGTATATTTTGGGGCAATAGGTGGAGCAGCAGCTTTAATATCTAAAA
GTATAAAAAAATCAGAAGTTATAGCTTATGAGGACTTAGATTCAGAAGCTATCAGAAAGTTAGAAGTAGAAGATCTGCCC
GTTACTGTTATAATAGATTCTAAAGGAAATAATTTATATGAGGATGGATTGGAAGATTATTTGAAAAGTTTATAA

Upstream 100 bases:

>100_bases
AAATTATAGTTTATATTATTTATATATTCTATGTGTAAAATAAATAAATATAACCTCCTTTAATATATTCTAAAGGAGAT
ATAAAGAGCAGGTGATTTTA

Downstream 100 bases:

>100_bases
TAATGTAGAGATTTAAACGCTAATAATATTAAATATTTACTTTTTAAATGTAGGATAGAGGGGTATATAATGAGAAAGGA
TAATAAATTTAGAAAGGGTT

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIYYVGPTPAKPGMEIGAAGPTT
SYRMDTYTPKLLNLGLKGMIGKGRRSKEVIESIVKNKAVYFGAIGGAAALISKSIKKSEVIAYEDLDSEAIRKLEVEDLP
VTVIIDSKGNNLYEDGLEDYLKSL

Sequences:

>Translated_184_residues
MKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIYYVGPTPAKPGMEIGAAGPTT
SYRMDTYTPKLLNLGLKGMIGKGRRSKEVIESIVKNKAVYFGAIGGAAALISKSIKKSEVIAYEDLDSEAIRKLEVEDLP
VTVIIDSKGNNLYEDGLEDYLKSL
>Mature_184_residues
MKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIYYVGPTPAKPGMEIGAAGPTT
SYRMDTYTPKLLNLGLKGMIGKGRRSKEVIESIVKNKAVYFGAIGGAAALISKSIKKSEVIAYEDLDSEAIRKLEVEDLP
VTVIIDSKGNNLYEDGLEDYLKSL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=179, Percent_Identity=38.5474860335196, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787897, Length=179, Percent_Identity=36.3128491620112, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1789443, Length=177, Percent_Identity=31.638418079096, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 20107; Mature: 20107

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIY
CEEEECCCCCHHHHHHCCCCCEEEEEECEEEHHHHHHHHHHHHHHHCCCCCEEECCCEEE
YVGPTPAKPGMEIGAAGPTTSYRMDTYTPKLLNLGLKGMIGKGRRSKEVIESIVKNKAVY
EECCCCCCCCCEECCCCCCCCEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEE
FGAIGGAAALISKSIKKSEVIAYEDLDSEAIRKLEVEDLPVTVIIDSKGNNLYEDGLEDY
EECCCHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHH
LKSL
HHCC
>Mature Secondary Structure
MKIKINTPLTEDKIKSLKAGDMVLITGTIYTARDAAHKRLIDALEKGRNLPFEVKNSIIY
CEEEECCCCCHHHHHHCCCCCEEEEEECEEEHHHHHHHHHHHHHHHCCCCCEEECCCEEE
YVGPTPAKPGMEIGAAGPTTSYRMDTYTPKLLNLGLKGMIGKGRRSKEVIESIVKNKAVY
EECCCCCCCCCEECCCCCCCCEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEE
FGAIGGAAALISKSIKKSEVIAYEDLDSEAIRKLEVEDLPVTVIIDSKGNNLYEDGLEDY
EECCCHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHH
LKSL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]