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The map label for this gene is nos [H]

Identifier: 152977623

GI number: 152977623

Start: 4021543

End: 4022622

Strand: Reverse

Name: nos [H]

Synonym: Bcer98_3961

Alternate gene names: 152977623

Gene position: 4022622-4021543 (Counterclockwise)

Preceding gene: 152977625

Following gene: 152977621

Centisome position: 98.42

GC content: 37.31

Gene sequence:

>1080_bases
ATGAATAAAATGAGGGTATTAATAGATGAGGCAAGAAAATTTATTACAACTTGCTATCAAGAACTCGGTAAAGAAAAACA
AATAGAAGAACGTATGAATCAAGTTGTGTTGGAAATCGAGCAGACAGGTACATATGAACATACTTTTGAGGAACTAGTTC
ATGGGGCACGGATGGCCTGGCGAAATAGTAATCGATGTATTGGAAGACTGTTTTGGAATAAAATGCATATATTGGATGCT
CGGGAGATCAATGATGAGGAAGGTGTATATCGTGCACTAATTCATCATATTCAATATGCGACAAATGGTGGGAAAATTAG
GCCAACCATTACGATTTTTAAGCAGTATAGAGATGAACAGAATCATATTCGCATCTATAATCACCAATTAATTCGCTATG
CAGGATATAAAACGGAAACAGGTGTGATTGGGGATCCGCATTCCTCTTCCTTTACATCTTTTTGCCAGAAACTAGGATGG
AGTGGGGAAGGAACGAATTTTGATGTGTTACCACTTGTATTCTCAATTGATGGGCAAGAACCTGTATATAAGGAGATTCC
GAAAGAAGAAGTAAAAGAAGTAAATATTGAACATCCAGAATACCCATTCTCCTCTCTTAGAGTGAAGTGGTATGGTGTAC
CGATGATTTCAGATATGCGTTTAGAAATTGGCGGAATTTCTTATACAGCAGCTCCTTTTAATGGATGGTATATGGGGACT
GAGATTGGTGCTCGTAATTTAGCTGATGTGGATCGTTATCATTTACTGCCAGCTATTGCAGAAATAATGAATTTAGATAC
AACTAGAAATAGTACGTTATGGAAAGATAAGGCGTTGGTCGAGTTAAATATAGCTGTCTTATATTCATTTAAAAAACAGG
GAGTAAGTATCGTTGATCACCATACAGCAGCGCAGCAATTTGCTCAATTTGAAAAACAAGAGGCGGCATGTGGTCGCGTT
GTGACTGGAAATTGGGTATGGCTAATTCCGCCATTATCGCCAGCTACCACTCATATATATCATAAACCTTATCCAAACGA
CATTTTGACCCCTAATTTTTTTCATAATGTTAAGAAATAA

Upstream 100 bases:

>100_bases
TTGTTTGTTAATAATGTAAAATAATTTGGTTTGTTCCTACGTTTCTTTTTGCTTATACTGTAAAATAGTAGCGTTTTGTT
GAAACAAAAGGAGATTTTTT

Downstream 100 bases:

>100_bases
AAATGAATCACGAATATCATTTTATCCAAAATGTAAAGAGCTCGTTCAAAACATAACTTTTGGACGAGCTCTTTTTGCAT
TTTTTAGTAGAAGCAAAGTT

Product: nitric-oxide synthase

Products: citrulline; nitric oxide; NADP+

Alternate protein names: NOSoxy-like protein [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNKMRVLIDEARKFITTCYQELGKEKQIEERMNQVVLEIEQTGTYEHTFEELVHGARMAWRNSNRCIGRLFWNKMHILDA
REINDEEGVYRALIHHIQYATNGGKIRPTITIFKQYRDEQNHIRIYNHQLIRYAGYKTETGVIGDPHSSSFTSFCQKLGW
SGEGTNFDVLPLVFSIDGQEPVYKEIPKEEVKEVNIEHPEYPFSSLRVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
EIGARNLADVDRYHLLPAIAEIMNLDTTRNSTLWKDKALVELNIAVLYSFKKQGVSIVDHHTAAQQFAQFEKQEAACGRV
VTGNWVWLIPPLSPATTHIYHKPYPNDILTPNFFHNVKK

Sequences:

>Translated_359_residues
MNKMRVLIDEARKFITTCYQELGKEKQIEERMNQVVLEIEQTGTYEHTFEELVHGARMAWRNSNRCIGRLFWNKMHILDA
REINDEEGVYRALIHHIQYATNGGKIRPTITIFKQYRDEQNHIRIYNHQLIRYAGYKTETGVIGDPHSSSFTSFCQKLGW
SGEGTNFDVLPLVFSIDGQEPVYKEIPKEEVKEVNIEHPEYPFSSLRVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
EIGARNLADVDRYHLLPAIAEIMNLDTTRNSTLWKDKALVELNIAVLYSFKKQGVSIVDHHTAAQQFAQFEKQEAACGRV
VTGNWVWLIPPLSPATTHIYHKPYPNDILTPNFFHNVKK
>Mature_359_residues
MNKMRVLIDEARKFITTCYQELGKEKQIEERMNQVVLEIEQTGTYEHTFEELVHGARMAWRNSNRCIGRLFWNKMHILDA
REINDEEGVYRALIHHIQYATNGGKIRPTITIFKQYRDEQNHIRIYNHQLIRYAGYKTETGVIGDPHSSSFTSFCQKLGW
SGEGTNFDVLPLVFSIDGQEPVYKEIPKEEVKEVNIEHPEYPFSSLRVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
EIGARNLADVDRYHLLPAIAEIMNLDTTRNSTLWKDKALVELNIAVLYSFKKQGVSIVDHHTAAQQFAQFEKQEAACGRV
VTGNWVWLIPPLSPATTHIYHKPYPNDILTPNFFHNVKK

Specific function: Catalyzes the production of nitric oxide [H]

COG id: COG4362

COG function: function code PE; Nitric oxide synthase, oxygenase domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NOS family. Bacterial NOS oxygenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI10835173, Length=351, Percent_Identity=45.014245014245, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI24041029, Length=357, Percent_Identity=43.1372549019608, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI40254422, Length=351, Percent_Identity=41.8803418803419, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI231571356, Length=351, Percent_Identity=41.8803418803419, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI231571314, Length=351, Percent_Identity=41.8803418803419, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI231571329, Length=351, Percent_Identity=41.8803418803419, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI78706866, Length=355, Percent_Identity=43.3802816901408, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI78706868, Length=355, Percent_Identity=43.3802816901408, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI78706882, Length=355, Percent_Identity=43.3802816901408, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI78706870, Length=355, Percent_Identity=43.3802816901408, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI78706878, Length=355, Percent_Identity=43.3802816901408, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI78706880, Length=355, Percent_Identity=43.3802816901408, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24583543, Length=355, Percent_Identity=43.3802816901408, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI78706876, Length=355, Percent_Identity=43.3802816901408, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI78706872, Length=153, Percent_Identity=42.483660130719, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017142
- InterPro:   IPR004030 [H]

Pfam domain/function: PF02898 NO_synthase [H]

EC number: 1.14.13.39

Molecular weight: Translated: 41540; Mature: 41540

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS60001 NOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMRVLIDEARKFITTCYQELGKEKQIEERMNQVVLEIEQTGTYEHTFEELVHGARMAW
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHHHHHHH
RNSNRCIGRLFWNKMHILDAREINDEEGVYRALIHHIQYATNGGKIRPTITIFKQYRDEQ
CCCCCEEHHHHHHHHHEEEHHHCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCC
NHIRIYNHQLIRYAGYKTETGVIGDPHSSSFTSFCQKLGWSGEGTNFDVLPLVFSIDGQE
CCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
PVYKEIPKEEVKEVNIEHPEYPFSSLRVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
CHHHHCCHHHHHHCCCCCCCCCHHHEEEEEECCCEEECCEEEECCEEEEECCCCCEEEEC
EIGARNLADVDRYHLLPAIAEIMNLDTTRNSTLWKDKALVELNIAVLYSFKKQGVSIVDH
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEEEEEEEHHHCCCEEEHH
HTAAQQFAQFEKQEAACGRVVTGNWVWLIPPLSPATTHIYHKPYPNDILTPNFFHNVKK
HHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MNKMRVLIDEARKFITTCYQELGKEKQIEERMNQVVLEIEQTGTYEHTFEELVHGARMAW
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHHHHHHH
RNSNRCIGRLFWNKMHILDAREINDEEGVYRALIHHIQYATNGGKIRPTITIFKQYRDEQ
CCCCCEEHHHHHHHHHEEEHHHCCCCHHHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCC
NHIRIYNHQLIRYAGYKTETGVIGDPHSSSFTSFCQKLGWSGEGTNFDVLPLVFSIDGQE
CCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
PVYKEIPKEEVKEVNIEHPEYPFSSLRVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
CHHHHCCHHHHHHCCCCCCCCCHHHEEEEEECCCEEECCEEEECCEEEEECCCCCEEEEC
EIGARNLADVDRYHLLPAIAEIMNLDTTRNSTLWKDKALVELNIAVLYSFKKQGVSIVDH
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEEEEEEEHHHCCCEEEHH
HTAAQQFAQFEKQEAACGRVVTGNWVWLIPPLSPATTHIYHKPYPNDILTPNFFHNVKK
HHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-arginine; NADPH; H+; O2

Specific reaction: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 11856757; 12220171 [H]