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The map label for this gene is mdeA [H]

Identifier: 152977587

GI number: 152977587

Start: 3978229

End: 3979527

Strand: Reverse

Name: mdeA [H]

Synonym: Bcer98_3925

Alternate gene names: 152977587

Gene position: 3979527-3978229 (Counterclockwise)

Preceding gene: 152977588

Following gene: 152977586

Centisome position: 97.37

GC content: 38.88

Gene sequence:

>1299_bases
ATGAGCGAATCATGGGGAAAAGGAACGATCTGTGTGCAAGGGGGATATACACCAAAGAATGGGGAGCCGCGCGTTCTACC
ACTTTATCAAAGTACAACATATAAATATGACACCTCAGATGATTTGGCGGCGCTTTTTAATTTAGAAGCAGAAGGGTATA
TCTATACGCGTATTGGGAATCCGACAATTGCAGCATTTGAGCAGAAGTTAGCAGAACTAGAGGGTGGTGTCGGTGCTGTT
GCAACAGCTTCCGGTCAGGCTGCTATTATGCTTGCTATTTTAAATATTTGTAGCAGTGGTGACCATTTACTTTGTTCTTC
AACTGTTTATGGAGGAACTTTTAACTTATTTGGAGTTAGTTTGCGTAAGCTTGGAATTGATGTTACATTTTTTAATCCGA
ACTTATCCGCTGATGAAATTGTGGCGCTTGCCAATGATAAAACAAAGCTTATTTATGCAGAATCACTTGGAAATCCAGCT
ATGAACGTTTTGAATTTCAAGGAATTTTCTCGTGCTGCAAAAGAACTAGACGTACCGTTTATTGTTGATAACACATTAGC
AACGCCATATTTATGTCAGGCGTTTGAGCATGGAGCAAATATTGTGGTCCATTCTACAACAAAGTATATTGATGGGCATG
CAAGCTCATTAGGTGGCGTTGTAATTGATGGAGGGAATTTTGATTGGACGAATGGAAAGTATCCCGAACTTGTAGAACCT
GACTCAAGTTATCATGGTGTAAGTTATGTGAAGGATTTTGGGGAAGCAGCATATATTGTAAAAGCGCGTGTTCAATTGTT
AAGAGACTATGGAAATTGTATGAGCCCCTTCAATGCATATATTAGCAATATTGGTTTAGAAACGTTGCATTTGAGAATGG
AACGCCATAGTGAAAATGCTCTTACAATTGCAAAGTGGCTTGCTAAGCATGAGCGTATTGAGTGGATTAATTATCCAGGA
TTAGAGGGTAACGAAAATTATCCATTGGCACAAAAATACTTATCGAAAGGTGCAAGTGGAGTTTTAACGTTTGGAATTAA
AGGCGGACTAGAAGCGGCGAAAGAATTTATAGCAAATGTGAATTTGGCAACGCTTGTAACACATGTTGCAGATGCAAGAA
CTTGTGTTATTCACCCTGCTAGTACAACACATAGACAGTTAACGGAAGAAGATCAACGTTTAGCCGGTGTTACGTCAGAT
TTAATTCGTTTATCAGTTGGTATAGAAGATGTTTCTGATATTATTGCTGATTTGGAAGCAGCTTTAATTGGAGGCAAAAC
AAATGCCAATCATAATTGA

Upstream 100 bases:

>100_bases
GATAAGAAGGAATTTTAGAGCGTGTTTTTAAACTGCTTCTTTCTCATTCAGGAAAGAAGCAGTTTTTTTATTTGTTTTAT
ATAAGAAGGGAGTATGAGAA

Downstream 100 bases:

>100_bases
CAAGGATTTACCAGCTCGTAAAGTGTTGCAAGAAGAAAATATTTTTACCATGACAAAGGAGCGAGCAGAAAAACAAGATA
TTAGAGCTTTAAAAATTGCA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTIAAFEQKLAELEGGVGAV
ATASGQAAIMLAILNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLSADEIVALANDKTKLIYAESLGNPA
MNVLNFKEFSRAAKELDVPFIVDNTLATPYLCQAFEHGANIVVHSTTKYIDGHASSLGGVVIDGGNFDWTNGKYPELVEP
DSSYHGVSYVKDFGEAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALTIAKWLAKHERIEWINYPG
LEGNENYPLAQKYLSKGASGVLTFGIKGGLEAAKEFIANVNLATLVTHVADARTCVIHPASTTHRQLTEEDQRLAGVTSD
LIRLSVGIEDVSDIIADLEAALIGGKTNANHN

Sequences:

>Translated_432_residues
MSESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTIAAFEQKLAELEGGVGAV
ATASGQAAIMLAILNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLSADEIVALANDKTKLIYAESLGNPA
MNVLNFKEFSRAAKELDVPFIVDNTLATPYLCQAFEHGANIVVHSTTKYIDGHASSLGGVVIDGGNFDWTNGKYPELVEP
DSSYHGVSYVKDFGEAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALTIAKWLAKHERIEWINYPG
LEGNENYPLAQKYLSKGASGVLTFGIKGGLEAAKEFIANVNLATLVTHVADARTCVIHPASTTHRQLTEEDQRLAGVTSD
LIRLSVGIEDVSDIIADLEAALIGGKTNANHN
>Mature_431_residues
SESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGNPTIAAFEQKLAELEGGVGAVA
TASGQAAIMLAILNICSSGDHLLCSSTVYGGTFNLFGVSLRKLGIDVTFFNPNLSADEIVALANDKTKLIYAESLGNPAM
NVLNFKEFSRAAKELDVPFIVDNTLATPYLCQAFEHGANIVVHSTTKYIDGHASSLGGVVIDGGNFDWTNGKYPELVEPD
SSYHGVSYVKDFGEAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENALTIAKWLAKHERIEWINYPGL
EGNENYPLAQKYLSKGASGVLTFGIKGGLEAAKEFIANVNLATLVTHVADARTCVIHPASTTHRQLTEEDQRLAGVTSDL
IRLSVGIEDVSDIIADLEAALIGGKTNANHN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=403, Percent_Identity=29.2803970223325, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI25453487, Length=402, Percent_Identity=26.865671641791, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI299473759, Length=403, Percent_Identity=25.8064516129032, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1790375, Length=395, Percent_Identity=31.8987341772152, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789383, Length=373, Percent_Identity=28.4182305630027, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=30.8823529411765, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=407, Percent_Identity=31.2039312039312, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=407, Percent_Identity=31.9410319410319, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI193202265, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=42.2330097087379, Blast_Score=319, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6319307, Length=403, Percent_Identity=32.258064516129, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=29.6969696969697, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=438, Percent_Identity=24.8858447488584, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=406, Percent_Identity=24.384236453202, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=31.5165876777251, Blast_Score=192, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46756; Mature: 46625

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGN
CCCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHEECCCCCEEEEEECC
PTIAAFEQKLAELEGGVGAVATASGQAAIMLAILNICSSGDHLLCSSTVYGGTFNLFGVS
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECEECCCCHHHHHHH
LRKLGIDVTFFNPNLSADEIVALANDKTKLIYAESLGNPAMNVLNFKEFSRAAKELDVPF
HHHHCEEEEEECCCCCHHHEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCE
IVDNTLATPYLCQAFEHGANIVVHSTTKYIDGHASSLGGVVIDGGNFDWTNGKYPELVEP
EECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
DSSYHGVSYVKDFGEAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENA
CCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEHHHCCCCH
LTIAKWLAKHERIEWINYPGLEGNENYPLAQKYLSKGASGVLTFGIKGGLEAAKEFIANV
HHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
NLATLVTHVADARTCVIHPASTTHRQLTEEDQRLAGVTSDLIRLSVGIEDVSDIIADLEA
CHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
ALIGGKTNANHN
HHCCCCCCCCCC
>Mature Secondary Structure 
SESWGKGTICVQGGYTPKNGEPRVLPLYQSTTYKYDTSDDLAALFNLEAEGYIYTRIGN
CCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHEECCCCCEEEEEECC
PTIAAFEQKLAELEGGVGAVATASGQAAIMLAILNICSSGDHLLCSSTVYGGTFNLFGVS
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECEECCCCHHHHHHH
LRKLGIDVTFFNPNLSADEIVALANDKTKLIYAESLGNPAMNVLNFKEFSRAAKELDVPF
HHHHCEEEEEECCCCCHHHEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCE
IVDNTLATPYLCQAFEHGANIVVHSTTKYIDGHASSLGGVVIDGGNFDWTNGKYPELVEP
EECCCCCCHHHHHHHHCCCCEEEECCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
DSSYHGVSYVKDFGEAAYIVKARVQLLRDYGNCMSPFNAYISNIGLETLHLRMERHSENA
CCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEHHHCCCCH
LTIAKWLAKHERIEWINYPGLEGNENYPLAQKYLSKGASGVLTFGIKGGLEAAKEFIANV
HHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
NLATLVTHVADARTCVIHPASTTHRQLTEEDQRLAGVTSDLIRLSVGIEDVSDIIADLEA
CHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
ALIGGKTNANHN
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]