Click here to switch to the map view.

The map label for this gene is yfmO [H]

Identifier: 152977549

GI number: 152977549

Start: 3944262

End: 3945407

Strand: Reverse

Name: yfmO [H]

Synonym: Bcer98_3885

Alternate gene names: 152977549

Gene position: 3945407-3944262 (Counterclockwise)

Preceding gene: 152977550

Following gene: 152977548

Centisome position: 96.53

GC content: 38.13

Gene sequence:

>1146_bases
ATGGGAAGATTCATTACAGTATTGGCTACATTCTTGGCTTTCTCAGGTATTGGGGTTGTCGACCCTATTTTGCCAATTAT
AGCTGAGAAAATTGGTGCCACACATTGGCAAGTAGAAATGCTGTTTACAGCGTATATTTTAACGATGGCAATTATGATGT
TACCAGCAGGAATCTTTGCTTCCAGATTTGGAGATAAGCGAATGATGACAATTGGTCTTGCGATTGTAACTGTTTTTGCA
TTTACATGTGCTATATCACAAACAATTGCGCAATTATCTCTTTTCCGTGCAGGATGGGGACTAGGGAACGCGATGTTTTT
TGCAACAGCAATGACATTATTGATTGCTCTAAGTCAAGAAGTTCATGAGGCAGTCGGTTTATATGAGGCAGCAATTGGTT
TCGGTATGGCTGGCGGACCGTTATTAGGTGGTATATTAGGCGGATATTCATGGCGTTACCCATTTTTTGCAACGAGCTTT
TTCATTTTCTTAGCATTTATTTTAGTATTCTTTTTTGTAAAAGAGCCGGAGCGAAAAACGAAGCGTAAGGCAGCTGGTGT
AGGAGAATTACTTAACTTAGTAAAATATAAACCATTTATGCAAGGTGCGATTTCAGGTATGTTATATTACTATGGTTTCT
TCGTTGTGCTAGCGTATTCACCACTTATTATGCATGTATCAGCAATCCAACTTGGATTTGTATTTTGCGGATGGGGGTTA
GCATTAGCATATGGTTCTGCGATATTAGCTCATAAATTAGAAGGGAAATATGAACCGAAGGCATTGCTACGTTTCAGCTT
ATTTGTATTTGCCATCCTATTAGTTGCATTATTCTTTGTTCAAATCATGTGGTTACAAATTGTTTTCATTGTTTTATCTG
GATTAGCATCTGGCTTAAATAACGCTCTATACACAAGCTATGTGATGGATATTTCACCTTATGAAAGATCGATTACATCG
GGTGTGTATAACTTTGTGCGCTGGTTAGGCGGAGCAATTGCTCCTATTTTATCGGGAATTATTGGTCATGCAATTTCACC
ACAGAGTCCGTTTTTAGTAGGTGGAATCGTTGTTTTAGTTGGATGTGTTATGATTTTTATTCCAGTTCGTCAATCAGCAG
AAATAGAGACAAAGGCCCTTTCTTAA

Upstream 100 bases:

>100_bases
GGAGCGAGTGAAAGCTGAATTAGAAGACAGCTTAGAGCGCGCACGTAATTTCTTAGAGAAAACAAAAGGAGAGTAAGTGA
ACATGGGGAGTAGACAGAAA

Downstream 100 bases:

>100_bases
GAAAGGGTTTTCTTTATATCGATATATAAGAGTCAGTTGACTTAAATATATTTTTAGTCTTTTTACACTTTTAACTATAC
TTTTCCGATGTTTTGTTTTA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MGRFITVLATFLAFSGIGVVDPILPIIAEKIGATHWQVEMLFTAYILTMAIMMLPAGIFASRFGDKRMMTIGLAIVTVFA
FTCAISQTIAQLSLFRAGWGLGNAMFFATAMTLLIALSQEVHEAVGLYEAAIGFGMAGGPLLGGILGGYSWRYPFFATSF
FIFLAFILVFFFVKEPERKTKRKAAGVGELLNLVKYKPFMQGAISGMLYYYGFFVVLAYSPLIMHVSAIQLGFVFCGWGL
ALAYGSAILAHKLEGKYEPKALLRFSLFVFAILLVALFFVQIMWLQIVFIVLSGLASGLNNALYTSYVMDISPYERSITS
GVYNFVRWLGGAIAPILSGIIGHAISPQSPFLVGGIVVLVGCVMIFIPVRQSAEIETKALS

Sequences:

>Translated_381_residues
MGRFITVLATFLAFSGIGVVDPILPIIAEKIGATHWQVEMLFTAYILTMAIMMLPAGIFASRFGDKRMMTIGLAIVTVFA
FTCAISQTIAQLSLFRAGWGLGNAMFFATAMTLLIALSQEVHEAVGLYEAAIGFGMAGGPLLGGILGGYSWRYPFFATSF
FIFLAFILVFFFVKEPERKTKRKAAGVGELLNLVKYKPFMQGAISGMLYYYGFFVVLAYSPLIMHVSAIQLGFVFCGWGL
ALAYGSAILAHKLEGKYEPKALLRFSLFVFAILLVALFFVQIMWLQIVFIVLSGLASGLNNALYTSYVMDISPYERSITS
GVYNFVRWLGGAIAPILSGIIGHAISPQSPFLVGGIVVLVGCVMIFIPVRQSAEIETKALS
>Mature_380_residues
GRFITVLATFLAFSGIGVVDPILPIIAEKIGATHWQVEMLFTAYILTMAIMMLPAGIFASRFGDKRMMTIGLAIVTVFAF
TCAISQTIAQLSLFRAGWGLGNAMFFATAMTLLIALSQEVHEAVGLYEAAIGFGMAGGPLLGGILGGYSWRYPFFATSFF
IFLAFILVFFFVKEPERKTKRKAAGVGELLNLVKYKPFMQGAISGMLYYYGFFVVLAYSPLIMHVSAIQLGFVFCGWGLA
LAYGSAILAHKLEGKYEPKALLRFSLFVFAILLVALFFVQIMWLQIVFIVLSGLASGLNNALYTSYVMDISPYERSITSG
VYNFVRWLGGAIAPILSGIIGHAISPQSPFLVGGIVVLVGCVMIFIPVRQSAEIETKALS

Specific function: Acts to efflux copper or a copper complex. It is possible that yfmO could contribute to copper resistance [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=189, Percent_Identity=29.1005291005291, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41517; Mature: 41386

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRFITVLATFLAFSGIGVVDPILPIIAEKIGATHWQVEMLFTAYILTMAIMMLPAGIFA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRFGDKRMMTIGLAIVTVFAFTCAISQTIAQLSLFRAGWGLGNAMFFATAMTLLIALSQE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VHEAVGLYEAAIGFGMAGGPLLGGILGGYSWRYPFFATSFFIFLAFILVFFFVKEPERKT
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
KRKAAGVGELLNLVKYKPFMQGAISGMLYYYGFFVVLAYSPLIMHVSAIQLGFVFCGWGL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAYGSAILAHKLEGKYEPKALLRFSLFVFAILLVALFFVQIMWLQIVFIVLSGLASGLN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALYTSYVMDISPYERSITSGVYNFVRWLGGAIAPILSGIIGHAISPQSPFLVGGIVVLV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GCVMIFIPVRQSAEIETKALS
HHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure 
GRFITVLATFLAFSGIGVVDPILPIIAEKIGATHWQVEMLFTAYILTMAIMMLPAGIFA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRFGDKRMMTIGLAIVTVFAFTCAISQTIAQLSLFRAGWGLGNAMFFATAMTLLIALSQE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VHEAVGLYEAAIGFGMAGGPLLGGILGGYSWRYPFFATSFFIFLAFILVFFFVKEPERKT
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
KRKAAGVGELLNLVKYKPFMQGAISGMLYYYGFFVVLAYSPLIMHVSAIQLGFVFCGWGL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAYGSAILAHKLEGKYEPKALLRFSLFVFAILLVALFFVQIMWLQIVFIVLSGLASGLN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NALYTSYVMDISPYERSITSGVYNFVRWLGGAIAPILSGIIGHAISPQSPFLVGGIVVLV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GCVMIFIPVRQSAEIETKALS
HHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]