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The map label for this gene is speE1 [H]

Identifier: 152977548

GI number: 152977548

Start: 3943270

End: 3944097

Strand: Reverse

Name: speE1 [H]

Synonym: Bcer98_3884

Alternate gene names: 152977548

Gene position: 3944097-3943270 (Counterclockwise)

Preceding gene: 152977549

Following gene: 152977547

Centisome position: 96.5

GC content: 36.96

Gene sequence:

>828_bases
ATGGAACTATGGTTCACTGAGAAACAAACAAAACATTTTGGAATTACGGCGCGTATTAACCGCACATTACATACGGAGCA
AACAGAATTTCAAAAGCTTGATATGGTAGAAACAGAAGAGTTCGGAAACATGCTGATTTTAGATGGTATGGTAATGACAA
CAGAAAAAGATGAGTTTGTCTATCATGAAATGGTAGCGCATGTTCCTTTATTTACACATCCAAACCCTGAGAACGTATTA
GTTGTTGGTGGTGGCGATGGTGGTGTCATTCGCGAAGTACTAAAACACCCAAGCGTGAAGAAAGCAACACTTGTTGAAAT
CGATGGGAAAGTAATTGAGTATTCTAAGAAATATTTGCCATCTATTGCAGGAGCATTAGATCATGAACGTGTGGAAGTAA
AAGTAGGAGATGGTTTCCTACATATCGCAGAAAGTGAAAATGAATACGATGTAATTATGGTTGACTCTACAGAGCCAGTC
GGACCAGCAGTTAACTTATTTACAAAAGGATTCTACGCTGGAATCTCTAAAGCGTTAAAAGAAGATGGGATTTTCGTTGC
ACAAACGGACAACCCTTGGTTTACACCAGAATTAATTACAAATGTAGTGAAAGATGTGAAAGAAATTTTCCCAATTACTC
GTTTGTATACAGCAAACATTCCAACGTACCCAAGTGGACTTTGGACATTTACAATTGGATCTAAAAAGTATGATCCACTA
GAAGTAAGTGAAGACCGTTTCCATGATATCGAAACGAAGTATTATACAAAAGAACTTCATAAAGCATCATTTGCTTTACC
GAAGTTTGTTGGAGATTTAATTAAGTAA

Upstream 100 bases:

>100_bases
ACACTTTTAACTATACTTTTCCGATGTTTTGTTTTATTTTTGTTAGGGGACATTAATGATGTCCCTATTATAAATTTGCA
ATGAAAGGCGTGATTGTACG

Downstream 100 bases:

>100_bases
GGGCGGGGAACGGTTAGAAGGGATAATCTCTTTTAGTCGCCGCTAAAGCATAAATAACTCATTTAAGGTGTTTGTCTATA
AGGCTTGAACATGCTAGTTA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKKYLPSIAGALDHERVEVKVGDGFLHIAESENEYDVIMVDSTEPV
GPAVNLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELITNVVKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKYDPL
EVSEDRFHDIETKYYTKELHKASFALPKFVGDLIK

Sequences:

>Translated_275_residues
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKKYLPSIAGALDHERVEVKVGDGFLHIAESENEYDVIMVDSTEPV
GPAVNLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELITNVVKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKYDPL
EVSEDRFHDIETKYYTKELHKASFALPKFVGDLIK
>Mature_275_residues
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKKYLPSIAGALDHERVEVKVGDGFLHIAESENEYDVIMVDSTEPV
GPAVNLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELITNVVKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKYDPL
EVSEDRFHDIETKYYTKELHKASFALPKFVGDLIK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=277, Percent_Identity=38.2671480144404, Blast_Score=232, Evalue=3e-61,
Organism=Escherichia coli, GI1786312, Length=269, Percent_Identity=37.1747211895911, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI25154106, Length=268, Percent_Identity=39.1791044776119, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI212645616, Length=222, Percent_Identity=42.7927927927928, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6325326, Length=277, Percent_Identity=39.3501805054152, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=35.6643356643357, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24645443, Length=274, Percent_Identity=40.1459854014599, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=40.7582938388626, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31160; Mature: 31160

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFV
CCCEECCCCCCEECEEEEECCEECCCHHHHHHHCCEEHHHHCCEEEECCEEEECCCHHHH
YHEMVAHVPLFTHPNPENVLVVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKKYLP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
SIAGALDHERVEVKVGDGFLHIAESENEYDVIMVDSTEPVGPAVNLFTKGFYAGISKALK
HHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EDGIFVAQTDNPWFTPELITNVVKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKYDPL
HCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEEECCCCCCCC
EVSEDRFHDIETKYYTKELHKASFALPKFVGDLIK
CCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFV
CCCEECCCCCCEECEEEEECCEECCCHHHHHHHCCEEHHHHCCEEEECCEEEECCCHHHH
YHEMVAHVPLFTHPNPENVLVVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKKYLP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
SIAGALDHERVEVKVGDGFLHIAESENEYDVIMVDSTEPVGPAVNLFTKGFYAGISKALK
HHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EDGIFVAQTDNPWFTPELITNVVKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKYDPL
HCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEEECCCCCCCC
EVSEDRFHDIETKYYTKELHKASFALPKFVGDLIK
CCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]