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The map label for this gene is prfA [H]

Identifier: 152977515

GI number: 152977515

Start: 3907398

End: 3908465

Strand: Reverse

Name: prfA [H]

Synonym: Bcer98_3850

Alternate gene names: 152977515

Gene position: 3908465-3907398 (Counterclockwise)

Preceding gene: 152977516

Following gene: 152977514

Centisome position: 95.63

GC content: 39.51

Gene sequence:

>1068_bases
GTGTTAGATCGTTTGCAAGCTGTAGAGGATCGTTATGAGAAGTTAAATGAGTTATTAAGTGACCCAGCGGTTATTAATGA
TTCTAATAAGCTTCGTGAGTATTCAAAGGAACAATCTGATATTCAAGAAACGGTAGAGGTGTATCGTGAGTATAAAGATG
TTCGTGAGCAATTGCAAGATGCGAAAGCGATGTTAGAAGATAAATTAGATGCTGAGATGCGTGAAATGGTAAAGGAAGAA
GTTTCAGATTTAGAGGTACAGGAGAAGGAATTATCAGATCGTTTGAAAATTTTACTTGTACCAAAAGATCCGAATGATGA
TAAGAACGTTATTGTTGAAATTCGCGGTGCAGCTGGTGGTGATGAGGCAGCTTTATTTGCTGGCGATTTATATCGTATGT
ATAGTCGTTATGCTGAGATGCAAGGATGGAAAACAGACATTATCGAAGCAAGTTACACAGAATTAGGTGGATATAAAGAG
ATTATCTTTATGATTAACGGTAAAGGCGCATATGCGAAGTTGAAATTCGAAAACGGTGCGCATCGTGTACAACGTGTTCC
AGAAACGGAATCTGGTGGACGCATTCACACATCTACAGCAACAGTAGCTGTATTACCAGAAGCGGAAGAAGTAGAAATCG
ATATTCATGAAAAGGATATTCGTGTGGATACATTTGCTTCTAGTGGTCCTGGTGGACAAAGTGTTAATACAACAATGTCA
GCGGTACGCTTAACTCACTTACCAACTGGTGTAGTTGTATCTTGTCAGGATGAAAAGTCACAAATTAAGAACAAAGAAAA
GGCAATGAAAGTATTACGTGCGCGTATTTATGATAAATTTAGGCAAGAAGCGCAAGCTGAGTATGATCAAAACCGTAAAC
AAGCTGTTGGTACGGGGGATCGTTCTGAGCGTATTCGTACGTATAACTTCCCGCAAAACCGTGTTACAGACCATCGAATC
GGCTTAACGATTCAAAAGCTAGATCAAATTTTACAAGGTAAGTTAGATGATTTCATCAACGCGCTAGTAATGGAAGATCA
GGCGAAGAAGATGGAGGCCGCTGAGTAA

Upstream 100 bases:

>100_bases
TTCTGAGCGAGCTGCCTTCGCTTTTAAATATAATTTGCGTTTTTTTTATTTTGTGCCATATACTATTGATATTAGATACT
AGGATGTTGAGGTGAATGAT

Downstream 100 bases:

>100_bases
TGCGTGTCTATGAAGCCCTGAAATGGGCTTCTTCTTTTTTACAAGAGAACGGGCGAGATGAAAATGCGGGAGAAATCGTC
CTTTGTCATGTATTAAACAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLDRLQAVEDRYEKLNELLSDPAVINDSNKLREYSKEQSDIQETVEVYREYKDVREQLQDAKAMLEDKLDAEMREMVKEE
VSDLEVQEKELSDRLKILLVPKDPNDDKNVIVEIRGAAGGDEAALFAGDLYRMYSRYAEMQGWKTDIIEASYTELGGYKE
IIFMINGKGAYAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILQGKLDDFINALVMEDQAKKMEAAE

Sequences:

>Translated_355_residues
MLDRLQAVEDRYEKLNELLSDPAVINDSNKLREYSKEQSDIQETVEVYREYKDVREQLQDAKAMLEDKLDAEMREMVKEE
VSDLEVQEKELSDRLKILLVPKDPNDDKNVIVEIRGAAGGDEAALFAGDLYRMYSRYAEMQGWKTDIIEASYTELGGYKE
IIFMINGKGAYAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILQGKLDDFINALVMEDQAKKMEAAE
>Mature_355_residues
MLDRLQAVEDRYEKLNELLSDPAVINDSNKLREYSKEQSDIQETVEVYREYKDVREQLQDAKAMLEDKLDAEMREMVKEE
VSDLEVQEKELSDRLKILLVPKDPNDDKNVIVEIRGAAGGDEAALFAGDLYRMYSRYAEMQGWKTDIIEASYTELGGYKE
IIFMINGKGAYAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDIRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILQGKLDDFINALVMEDQAKKMEAAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=44.7916666666667, Blast_Score=264, Evalue=8e-71,
Organism=Homo sapiens, GI34577120, Length=342, Percent_Identity=37.4269005847953, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=40.0966183574879, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=50.9803921568627, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=36.6863905325444, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=283, Percent_Identity=39.2226148409894, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=42.814371257485, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19921226, Length=334, Percent_Identity=38.622754491018, Blast_Score=231, Evalue=5e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40431; Mature: 40431

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLQAVEDRYEKLNELLSDPAVINDSNKLREYSKEQSDIQETVEVYREYKDVREQLQD
CCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEDKLDAEMREMVKEEVSDLEVQEKELSDRLKILLVPKDPNDDKNVIVEIRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCCCC
DEAALFAGDLYRMYSRYAEMQGWKTDIIEASYTELGGYKEIIFMINGKGAYAKLKFENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFRQEAQAEYDQNRKQAVGTGD
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAKKMEAAE
CCCCCEECCCCCCCCCCHHHCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDRLQAVEDRYEKLNELLSDPAVINDSNKLREYSKEQSDIQETVEVYREYKDVREQLQD
CCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEDKLDAEMREMVKEEVSDLEVQEKELSDRLKILLVPKDPNDDKNVIVEIRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCCCC
DEAALFAGDLYRMYSRYAEMQGWKTDIIEASYTELGGYKEIIFMINGKGAYAKLKFENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFRQEAQAEYDQNRKQAVGTGD
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAKKMEAAE
CCCCCEECCCCCCCCCCHHHCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]