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The map label for this gene is ywkE [H]

Identifier: 152977514

GI number: 152977514

Start: 3906547

End: 3907398

Strand: Reverse

Name: ywkE [H]

Synonym: Bcer98_3849

Alternate gene names: 152977514

Gene position: 3907398-3906547 (Counterclockwise)

Preceding gene: 152977515

Following gene: 152977513

Centisome position: 95.6

GC content: 38.38

Gene sequence:

>852_bases
ATGCGTGTCTATGAAGCCCTGAAATGGGCTTCTTCTTTTTTACAAGAGAACGGGCGAGATGAAAATGCGGGAGAAATCGT
CCTTTGTCATGTATTAAACACAAATCGAACGGGTTTAATGATGAATATGCGTGAAGAGATATCTAACGAACAAGAAAAGC
GTTTTAAAGAGTTGATTCATAAACATATTGATGGAATGCCTGTTCAATATTTAATGGGATATGAGATGTTTTATGGTCGA
TCCTTTTTTGTAAATGAGGAAGTGCTAATACCGCGCCCAGAAACAGAAGAACTTATAGTTGGTGTACTGGAGAGAATTCA
GCGTAATTTTGGTAATCAAGAGCTTCATGTAGCTGACATTGGAACAGGAAGTGGAGCTATTTCCATTACACTTGCATTAG
AGAATCAAAATCTTCATGTATATACGGTAGATATTGCTCAAGAGTCCATTGAAGTTGCCAAACAAAATGCAACATCATTG
GGAGCAAATGTGACATTTTATCACGGAGATTTATTATCACCATTTTATGAAACAGGGAAAAAATTAGACGTTGTTGTTTC
TAATCCTCCGTATATACCGGAGGAAGATTGGAAAGGATTATCTTCTGTTGTGAAAGAACATGAGCCGAAGCGAGCGCTTG
TTGGTGGAGCAGATGGTCTTGATTTTTATCGCCGTTTTATGGAAGAGTTGCCTCGTGTATTACAGAAAAAAGCGCTTGTT
GCTTTTGAAGTTGGTGTAGGGCAAGGAGAAGATGTAAAAGGGCTATTAGAAGATACTTTCCCACATGCTCATGTAGAAGT
GGTTTTCGATATTAATGGAAAAGATCGCATGGTCTTTGCGGAAATGGAGTAA

Upstream 100 bases:

>100_bases
CGATTCAAAAGCTAGATCAAATTTTACAAGGTAAGTTAGATGATTTCATCAACGCGCTAGTAATGGAAGATCAGGCGAAG
AAGATGGAGGCCGCTGAGTA

Downstream 100 bases:

>100_bases
GGCATAAGCCTTGCTCTATTTTTGTATAAAAAATTTACATAGATTTTGAATTTAATAGTTTAGACAGATGAAAGTCTGTC
CACACTGTTTCTAGAAGGGA

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLNTNRTGLMMNMREEISNEQEKRFKELIHKHIDGMPVQYLMGYEMFYGR
SFFVNEEVLIPRPETEELIVGVLERIQRNFGNQELHVADIGTGSGAISITLALENQNLHVYTVDIAQESIEVAKQNATSL
GANVTFYHGDLLSPFYETGKKLDVVVSNPPYIPEEDWKGLSSVVKEHEPKRALVGGADGLDFYRRFMEELPRVLQKKALV
AFEVGVGQGEDVKGLLEDTFPHAHVEVVFDINGKDRMVFAEME

Sequences:

>Translated_283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLNTNRTGLMMNMREEISNEQEKRFKELIHKHIDGMPVQYLMGYEMFYGR
SFFVNEEVLIPRPETEELIVGVLERIQRNFGNQELHVADIGTGSGAISITLALENQNLHVYTVDIAQESIEVAKQNATSL
GANVTFYHGDLLSPFYETGKKLDVVVSNPPYIPEEDWKGLSSVVKEHEPKRALVGGADGLDFYRRFMEELPRVLQKKALV
AFEVGVGQGEDVKGLLEDTFPHAHVEVVFDINGKDRMVFAEME
>Mature_283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLNTNRTGLMMNMREEISNEQEKRFKELIHKHIDGMPVQYLMGYEMFYGR
SFFVNEEVLIPRPETEELIVGVLERIQRNFGNQELHVADIGTGSGAISITLALENQNLHVYTVDIAQESIEVAKQNATSL
GANVTFYHGDLLSPFYETGKKLDVVVSNPPYIPEEDWKGLSSVVKEHEPKRALVGGADGLDFYRRFMEELPRVLQKKALV
AFEVGVGQGEDVKGLLEDTFPHAHVEVVFDINGKDRMVFAEME

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=273, Percent_Identity=28.9377289377289, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1787463, Length=248, Percent_Identity=31.4516129032258, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082085, Length=201, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324266, Length=249, Percent_Identity=26.5060240963855, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24582226, Length=239, Percent_Identity=25.5230125523013, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32079; Mature: 32079

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYEALKWASSFLQENGRDENAGEIVLCHVLNTNRTGLMMNMREEISNEQEKRFKELIH
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHCCHHHHHHHHHHH
KHIDGMPVQYLMGYEMFYGRSFFVNEEVLIPRPETEELIVGVLERIQRNFGNQELHVADI
HHHCCCCHHHHHHHHHHHCCEEEECCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
GTGSGAISITLALENQNLHVYTVDIAQESIEVAKQNATSLGANVTFYHGDLLSPFYETGK
CCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHCHHHCCCEEEEEECCHHHHHHHCCC
KLDVVVSNPPYIPEEDWKGLSSVVKEHEPKRALVGGADGLDFYRRFMEELPRVLQKKALV
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCEE
AFEVGVGQGEDVKGLLEDTFPHAHVEVVFDINGKDRMVFAEME
EEEECCCCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MRVYEALKWASSFLQENGRDENAGEIVLCHVLNTNRTGLMMNMREEISNEQEKRFKELIH
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHCCHHHHHHHHHHH
KHIDGMPVQYLMGYEMFYGRSFFVNEEVLIPRPETEELIVGVLERIQRNFGNQELHVADI
HHHCCCCHHHHHHHHHHHCCEEEECCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
GTGSGAISITLALENQNLHVYTVDIAQESIEVAKQNATSLGANVTFYHGDLLSPFYETGK
CCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHCHHHCCCEEEEEECCHHHHHHHCCC
KLDVVVSNPPYIPEEDWKGLSSVVKEHEPKRALVGGADGLDFYRRFMEELPRVLQKKALV
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCEE
AFEVGVGQGEDVKGLLEDTFPHAHVEVVFDINGKDRMVFAEME
EEEECCCCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]