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The map label for this gene is ndhF [H]

Identifier: 152977479

GI number: 152977479

Start: 3872646

End: 3874508

Strand: Reverse

Name: ndhF [H]

Synonym: Bcer98_3811

Alternate gene names: 152977479

Gene position: 3874508-3872646 (Counterclockwise)

Preceding gene: 152977480

Following gene: 152977478

Centisome position: 94.8

GC content: 42.08

Gene sequence:

>1863_bases
ATGATCGATTATGCATGGCTCATACCGCTTTTCCCGCTTGCTTCGTTTATGTTACTCATTTTATTTGGGAGGAAATTAAA
AGGAATTAGCAGCCTCCTTGGCAGCCTTTTCGCATTTCTTTCGCTAGTGGGTGCGGTAATCGTCTTGTTAGAACGCTTTT
CCTCAGAGAATGTCAAGCACACTTGGTTATGGCTAAAGGCTGGGAGTGTGGACATTTCATTTGGATTTGAGGTGAATGCA
TTGAATGCGTTAATGCTCCTTATTGTGACTACTGTCAGTTTTCTTGTACATGTGTATGCAAAAGGATATATGCAAGGCGA
CGAACGTTTGTCAGTCTTTTATGCGTATCTTGGATTATTTACATTTGCGATGCTTGGGCTTGTAATTTCAACAAATCTAT
TGCAACTTTACGTGTTTTGGGAGCTTGTTGGTCTCGGTTCCTTTTTATTAATTGGATTTTACTTTTTTAAAGAAGAGGCA
AAAGCAGCTGCCCAAAAAGCTTTTATTATGACGCGCATTGGCGATGCCTGCTTATTTATCGGGATGATTCTTCTTTTTTG
GCAAGCGGGCAGTTTTGATTATGATGTTATTTTTAAAGCAGTACAAACGGAGGCTATCTCTCCTTCTATCATTACACTTA
CAGCGCTTTTCATATTTATTGGTGCCGTGGGGAAATCGGGACAATTTCCATTGCATACGTGGCTACCGGATGCAATGGAA
GGGCCTACGCCAGTTTCAGCACTTATTCATGCGGCGACGATGGTCGCGGCCGGCGTCTATTTAGTTGCGGCGATGTTTCC
GCTTTTCGTAGCCAGCCCTGTAGCTATGCAGACCGTAGCAATTGTGGGAGGATTTACGGCGATTTTTGCGGCGTCCATTG
CTCTTGTACAAACAGATATAAAACGCATTCTAGCGTATTCTACAGTAAGTCAGCTCGGTTATATGATGCTTGCACTTGGT
TCAGCGGGATATGTCGCTAGTATCTTTCATTTAACGACACACGCCTTTTTTAAGGCACTTCTGTTCTTAGCGGCTGGAAG
TGTCATTCATGCGGTGCATACACAAAACATTCATGAGATGGGCGGCTTACAAAAGAAGATGAAAGTTACAGGACTACTCT
TTTTAGTCGGTACATTTGCAATTAGTGGCGTTCCGTTTTTTTCAGGCTTTTTTAGTAAAGACGAAATTTTAATGGCAACG
TGGATGCACGGCAATTATGTTTTATTTGTCCTCGCCATTTTAGCAACGTTCTTAACGTCCTTTTATATGTTTCGTTTATA
CTTTCTTGTCTTTACCGGAGAAGAGAGAGCGAAGAGGGAAGTACGTGAGTCTCCGCGGATTATGACATTGCCGATGACTG
CTCTTGGAGTATTCGCTGTTGCAATAGGATACATTCATACACCTTGGTTCGGAACATTTCTTGGAGATTGGCTCATAAAG
GATGTTTCATTTCAAGTAGCACAAGGACACGGGCCAGCTTGGATTATGGTCATAGCAACGCTCGTCTCTTTTGGAGGAAT
TCTCCTTGCCTATTTGATGTATAACAAGCGCTCTCTTTCTAGAGATTGGGCTGGGGGAGAAGGAACAATTCTATCTCATC
TACTTATAAAAAAATATTATATAGATGAAATATATCATGTGACGGTGTTGCCGATTGTAAAAGGGATTGCTTATACACTT
CACCTTTTTGAAGTATGTATTGTGGAGGGAATTGCAAAGCTCATCCAGAGCCTCGTTAAAGGGGGGAGTTACGGGGGATC
AAAACTTCAAAACGGTAATGTACAAGTGTACGGAACGGTGACTGCTATTTCTTTGGCAGCATTGGTTGTCATTTTGTTAT
ATACAGGGGGGGATTGGCAATGA

Upstream 100 bases:

>100_bases
CGCAATTTTGATTGCACTATATCGCAATCGTACAACTGTTAATATCGATGAGATGGATAAGTTGAAAGGTTAATTCTAAC
TGAAAAGGGGGAAGGAAACG

Downstream 100 bases:

>100_bases
GTGCTTTCTTATTATCTTTCTTCATTTTCTCCCCACTTCTTAGTATCTTCTTACTTCTATTTACACCAAAAACAAAGGTA
CGTGCGGTGCGCGGGATTGG

Product: NADH dehydrogenase subunit L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MIDYAWLIPLFPLASFMLLILFGRKLKGISSLLGSLFAFLSLVGAVIVLLERFSSENVKHTWLWLKAGSVDISFGFEVNA
LNALMLLIVTTVSFLVHVYAKGYMQGDERLSVFYAYLGLFTFAMLGLVISTNLLQLYVFWELVGLGSFLLIGFYFFKEEA
KAAAQKAFIMTRIGDACLFIGMILLFWQAGSFDYDVIFKAVQTEAISPSIITLTALFIFIGAVGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVAAMFPLFVASPVAMQTVAIVGGFTAIFAASIALVQTDIKRILAYSTVSQLGYMMLALG
SAGYVASIFHLTTHAFFKALLFLAAGSVIHAVHTQNIHEMGGLQKKMKVTGLLFLVGTFAISGVPFFSGFFSKDEILMAT
WMHGNYVLFVLAILATFLTSFYMFRLYFLVFTGEERAKREVRESPRIMTLPMTALGVFAVAIGYIHTPWFGTFLGDWLIK
DVSFQVAQGHGPAWIMVIATLVSFGGILLAYLMYNKRSLSRDWAGGEGTILSHLLIKKYYIDEIYHVTVLPIVKGIAYTL
HLFEVCIVEGIAKLIQSLVKGGSYGGSKLQNGNVQVYGTVTAISLAALVVILLYTGGDWQ

Sequences:

>Translated_620_residues
MIDYAWLIPLFPLASFMLLILFGRKLKGISSLLGSLFAFLSLVGAVIVLLERFSSENVKHTWLWLKAGSVDISFGFEVNA
LNALMLLIVTTVSFLVHVYAKGYMQGDERLSVFYAYLGLFTFAMLGLVISTNLLQLYVFWELVGLGSFLLIGFYFFKEEA
KAAAQKAFIMTRIGDACLFIGMILLFWQAGSFDYDVIFKAVQTEAISPSIITLTALFIFIGAVGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVAAMFPLFVASPVAMQTVAIVGGFTAIFAASIALVQTDIKRILAYSTVSQLGYMMLALG
SAGYVASIFHLTTHAFFKALLFLAAGSVIHAVHTQNIHEMGGLQKKMKVTGLLFLVGTFAISGVPFFSGFFSKDEILMAT
WMHGNYVLFVLAILATFLTSFYMFRLYFLVFTGEERAKREVRESPRIMTLPMTALGVFAVAIGYIHTPWFGTFLGDWLIK
DVSFQVAQGHGPAWIMVIATLVSFGGILLAYLMYNKRSLSRDWAGGEGTILSHLLIKKYYIDEIYHVTVLPIVKGIAYTL
HLFEVCIVEGIAKLIQSLVKGGSYGGSKLQNGNVQVYGTVTAISLAALVVILLYTGGDWQ
>Mature_620_residues
MIDYAWLIPLFPLASFMLLILFGRKLKGISSLLGSLFAFLSLVGAVIVLLERFSSENVKHTWLWLKAGSVDISFGFEVNA
LNALMLLIVTTVSFLVHVYAKGYMQGDERLSVFYAYLGLFTFAMLGLVISTNLLQLYVFWELVGLGSFLLIGFYFFKEEA
KAAAQKAFIMTRIGDACLFIGMILLFWQAGSFDYDVIFKAVQTEAISPSIITLTALFIFIGAVGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVAAMFPLFVASPVAMQTVAIVGGFTAIFAASIALVQTDIKRILAYSTVSQLGYMMLALG
SAGYVASIFHLTTHAFFKALLFLAAGSVIHAVHTQNIHEMGGLQKKMKVTGLLFLVGTFAISGVPFFSGFFSKDEILMAT
WMHGNYVLFVLAILATFLTSFYMFRLYFLVFTGEERAKREVRESPRIMTLPMTALGVFAVAIGYIHTPWFGTFLGDWLIK
DVSFQVAQGHGPAWIMVIATLVSFGGILLAYLMYNKRSLSRDWAGGEGTILSHLLIKKYYIDEIYHVTVLPIVKGIAYTL
HLFEVCIVEGIAKLIQSLVKGGSYGGSKLQNGNVQVYGTVTAISLAALVVILLYTGGDWQ

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=418, Percent_Identity=35.6459330143541, Blast_Score=255, Evalue=7e-68,
Organism=Escherichia coli, GI1788614, Length=562, Percent_Identity=40.9252669039146, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788829, Length=373, Percent_Identity=34.3163538873995, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788831, Length=338, Percent_Identity=31.9526627218935, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1788827, Length=427, Percent_Identity=29.03981264637, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI2367154, Length=429, Percent_Identity=24.9417249417249, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788613, Length=390, Percent_Identity=26.1538461538462, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI145693160, Length=265, Percent_Identity=27.1698113207547, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 68019; Mature: 68019

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYAWLIPLFPLASFMLLILFGRKLKGISSLLGSLFAFLSLVGAVIVLLERFSSENVKH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
TWLWLKAGSVDISFGFEVNALNALMLLIVTTVSFLVHVYAKGYMQGDERLSVFYAYLGLF
EEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TFAMLGLVISTNLLQLYVFWELVGLGSFLLIGFYFFKEEAKAAAQKAFIMTRIGDACLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMILLFWQAGSFDYDVIFKAVQTEAISPSIITLTALFIFIGAVGKSGQFPLHTWLPDAME
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC
GPTPVSALIHAATMVAAGVYLVAAMFPLFVASPVAMQTVAIVGGFTAIFAASIALVQTDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRILAYSTVSQLGYMMLALGSAGYVASIFHLTTHAFFKALLFLAAGSVIHAVHTQNIHEM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHH
GGLQKKMKVTGLLFLVGTFAISGVPFFSGFFSKDEILMATWMHGNYVLFVLAILATFLTS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
FYMFRLYFLVFTGEERAKREVRESPRIMTLPMTALGVFAVAIGYIHTPWFGTFLGDWLIK
HHHHHHHHHHCCCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DVSFQVAQGHGPAWIMVIATLVSFGGILLAYLMYNKRSLSRDWAGGEGTILSHLLIKKYY
HHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IDEIYHVTVLPIVKGIAYTLHLFEVCIVEGIAKLIQSLVKGGSYGGSKLQNGNVQVYGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHH
TAISLAALVVILLYTGGDWQ
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIDYAWLIPLFPLASFMLLILFGRKLKGISSLLGSLFAFLSLVGAVIVLLERFSSENVKH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
TWLWLKAGSVDISFGFEVNALNALMLLIVTTVSFLVHVYAKGYMQGDERLSVFYAYLGLF
EEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TFAMLGLVISTNLLQLYVFWELVGLGSFLLIGFYFFKEEAKAAAQKAFIMTRIGDACLFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMILLFWQAGSFDYDVIFKAVQTEAISPSIITLTALFIFIGAVGKSGQFPLHTWLPDAME
HHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC
GPTPVSALIHAATMVAAGVYLVAAMFPLFVASPVAMQTVAIVGGFTAIFAASIALVQTDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRILAYSTVSQLGYMMLALGSAGYVASIFHLTTHAFFKALLFLAAGSVIHAVHTQNIHEM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHH
GGLQKKMKVTGLLFLVGTFAISGVPFFSGFFSKDEILMATWMHGNYVLFVLAILATFLTS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
FYMFRLYFLVFTGEERAKREVRESPRIMTLPMTALGVFAVAIGYIHTPWFGTFLGDWLIK
HHHHHHHHHHCCCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DVSFQVAQGHGPAWIMVIATLVSFGGILLAYLMYNKRSLSRDWAGGEGTILSHLLIKKYY
HHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IDEIYHVTVLPIVKGIAYTLHLFEVCIVEGIAKLIQSLVKGGSYGGSKLQNGNVQVYGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHH
TAISLAALVVILLYTGGDWQ
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]