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The map label for this gene is dapF [H]

Identifier: 152977227

GI number: 152977227

Start: 3611138

End: 3612004

Strand: Reverse

Name: dapF [H]

Synonym: Bcer98_3545

Alternate gene names: 152977227

Gene position: 3612004-3611138 (Counterclockwise)

Preceding gene: 152977228

Following gene: 152977226

Centisome position: 88.38

GC content: 39.22

Gene sequence:

>867_bases
ATGAGCCAATTTTCTTTTACAAAAATGCATGGTCTTGGAAATAGCTATATATATGTAAATATGTTTGAAGAACATATTCC
AGAGGAAGAACTAGCTCTTGTAGCAGAGAAAGTTTCGAACAGAAATACCGGCATTGGGGCTGATGGAATGATTTTAATTT
GTCCATCTGAAGTAGCACCAGTAAAAATGCGTATGTTTAATAATGATGGCTCAGAGGGAAAGAGTTGTGGAAATGGGCTT
CGCTGTGTAGCGAAATATGCATATGAGCATAAATTAGTAGAAGAAACGATTTTTACGATTGAAACATTAGCTGGTATTGT
AACAGCTGAGGTAACAGTCGAGAATGATATTGTTACACTCGTTAAAATTGATATGGGGGCACCTCGTTTAACACGTGCGG
AGTTACCGATGCTTGGAGAAGGAGAAACGCCGTTTATTCGTGAGGACTTTCTATTTCATAATCAACGTTATGCATTTACA
GCAGTTTCTATGGGAAATCCACATGCTGTAATTTTTGTTGATGATGTAGAAAAGGCACCTCTTACAACACTGGGACCTGT
ACTTGAGAATCATGAAATGTTTCCAGAACGGGTAAATGTTGAGTTCATTGAAATTTTGAATGAAACAGAGATGAATTTCC
GCGTATGGGAACGTGGATCAGGTGTAACGCAAGCATGTGGAACGGGAGCGTGTGCATCTGTTGTAGCAGCAATCTTAAAT
GGAAAAATGGAGCGCGGTAAAGAAATTACGGTTCATTTAGCTGGCGGTGACTTAATGATTACGTGGACAGAAGAAGGAAC
TGTAATGATGAAAGGACCAGCAGAAGTGATTTGTCACGGAGTGTATGAGTACAAGATAGAAGCATAA

Upstream 100 bases:

>100_bases
AAAACGTTGTCAAATATGAGAAACGGGGGACATTCATATTGTGTTCCAAAAGCATGCATTTTATACTTAAAACAATAAAT
TATTTTTGGAGTGAATCAGA

Downstream 100 bases:

>100_bases
AGATGTATAATAGAATAAGAAAGGAAGGTGGATTTTATGATTACTGTAACACAACAAGCAGCATTTCAAATTAAGGATAT
GTTAAAAGATGCTGAAGATG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSQFSFTKMHGLGNSYIYVNMFEEHIPEEELALVAEKVSNRNTGIGADGMILICPSEVAPVKMRMFNNDGSEGKSCGNGL
RCVAKYAYEHKLVEETIFTIETLAGIVTAEVTVENDIVTLVKIDMGAPRLTRAELPMLGEGETPFIREDFLFHNQRYAFT
AVSMGNPHAVIFVDDVEKAPLTTLGPVLENHEMFPERVNVEFIEILNETEMNFRVWERGSGVTQACGTGACASVVAAILN
GKMERGKEITVHLAGGDLMITWTEEGTVMMKGPAEVICHGVYEYKIEA

Sequences:

>Translated_288_residues
MSQFSFTKMHGLGNSYIYVNMFEEHIPEEELALVAEKVSNRNTGIGADGMILICPSEVAPVKMRMFNNDGSEGKSCGNGL
RCVAKYAYEHKLVEETIFTIETLAGIVTAEVTVENDIVTLVKIDMGAPRLTRAELPMLGEGETPFIREDFLFHNQRYAFT
AVSMGNPHAVIFVDDVEKAPLTTLGPVLENHEMFPERVNVEFIEILNETEMNFRVWERGSGVTQACGTGACASVVAAILN
GKMERGKEITVHLAGGDLMITWTEEGTVMMKGPAEVICHGVYEYKIEA
>Mature_287_residues
SQFSFTKMHGLGNSYIYVNMFEEHIPEEELALVAEKVSNRNTGIGADGMILICPSEVAPVKMRMFNNDGSEGKSCGNGLR
CVAKYAYEHKLVEETIFTIETLAGIVTAEVTVENDIVTLVKIDMGAPRLTRAELPMLGEGETPFIREDFLFHNQRYAFTA
VSMGNPHAVIFVDDVEKAPLTTLGPVLENHEMFPERVNVEFIEILNETEMNFRVWERGSGVTQACGTGACASVVAAILNG
KMERGKEITVHLAGGDLMITWTEEGTVMMKGPAEVICHGVYEYKIEA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=277, Percent_Identity=37.5451263537906, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31778; Mature: 31647

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFSFTKMHGLGNSYIYVNMFEEHIPEEELALVAEKVSNRNTGIGADGMILICPSEVAP
CCCCCCCHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
VKMRMFNNDGSEGKSCGNGLRCVAKYAYEHKLVEETIFTIETLAGIVTAEVTVENDIVTL
EEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEE
VKIDMGAPRLTRAELPMLGEGETPFIREDFLFHNQRYAFTAVSMGNPHAVIFVDDVEKAP
EEEECCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCC
LTTLGPVLENHEMFPERVNVEFIEILNETEMNFRVWERGSGVTQACGTGACASVVAAILN
CHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHC
GKMERGKEITVHLAGGDLMITWTEEGTVMMKGPAEVICHGVYEYKIEA
CCCCCCCEEEEEEECCEEEEEEECCCEEEEECCHHHHEECEEEEEECC
>Mature Secondary Structure 
SQFSFTKMHGLGNSYIYVNMFEEHIPEEELALVAEKVSNRNTGIGADGMILICPSEVAP
CCCCCCHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
VKMRMFNNDGSEGKSCGNGLRCVAKYAYEHKLVEETIFTIETLAGIVTAEVTVENDIVTL
EEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEE
VKIDMGAPRLTRAELPMLGEGETPFIREDFLFHNQRYAFTAVSMGNPHAVIFVDDVEKAP
EEEECCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCC
LTTLGPVLENHEMFPERVNVEFIEILNETEMNFRVWERGSGVTQACGTGACASVVAAILN
CHHHHHHHHCCCCCCHHCCCCEEEECCCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHC
GKMERGKEITVHLAGGDLMITWTEEGTVMMKGPAEVICHGVYEYKIEA
CCCCCCCEEEEEEECCEEEEEEECCCEEEEECCHHHHEECEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA