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The map label for this gene is ldh [H]

Identifier: 152977188

GI number: 152977188

Start: 3568086

End: 3569033

Strand: Reverse

Name: ldh [H]

Synonym: Bcer98_3505

Alternate gene names: 152977188

Gene position: 3569033-3568086 (Counterclockwise)

Preceding gene: 152977191

Following gene: 152977187

Centisome position: 87.33

GC content: 37.55

Gene sequence:

>948_bases
ATGAAAAAAGGCATTAATCGTGTAGTATTAGTAGGAACAGGAGCTGTAGGTTGTAGTTACGCTTATTCCATGATTAACCA
AGGTGTAGCTGAAGAATTCGTGCTTGTAGATGTGAATGAAGCAAAGGCAGAAGGAGAAGCGATGGACTTAAGCCATGCGG
TACCATTTGCTCCATCACCAACAAAGATTTGGAAAGGTAGCTATGAAGATTGTAAGGATGCTGACATTGTCTGTATCACA
GCTGGATTACCACAAAAACCAGGTGAAACTCGTTTAGATTTAGTGGAGAAAAATGCAAAAATCTTTAAGCAAATTGTTCG
TAGTATTATGGATACTGGTTTCGATGGGATTTTCTTAATCGCAACTAACCCAGTTGATATTTTAACTTATGTAACTTGGA
AAGAATCTGGATTACCAAAAGAGCGTGTAATTGGTTCTGGTACAACTCTTGACTCTGCTCGTTTCCGCTACATGTTGGGT
GATTACTTCGATGTGGATCCACGCAATGTTCATGCATATATCATTGGGGAACATGGTGATACTGAACTTCCAGTTTGGAG
TCATGCGACAATAGGTGTTCAAAAAATTGAAAAAATTATGTCTGATAACAAAGAAAAATACAGCCAAAAAGATTTAGAAG
ATATCTTTGTCAATGTTCGTGATGCTGCTTATCATATCATCGAGCGTAAAGGCGCAACTTACTATGGAATCGGTATGTCA
CTACTTCGTGTAACAAAAGCAATTTTAAACAACGAGAACAGCGTATTAACTGTATCTGCATACCTTGAAGGCCAATATGG
TGAGAAAGATGCTTATGTAGGTGTTCCTGCTATTATTAATCGTCAAGGTGTACGTGAAATTGTTGAGCTCGAGTTGAATG
AAGAAGAAAAAGCAAAATTCGCTCATTCTGTAAAAGTATTGAAAGAAACAATGGCACCAGTATTATAA

Upstream 100 bases:

>100_bases
ACAATATGAGTGTAAGGAAGTTAAACAACATTAAATTAGTACTTTAATTAATTATTAAAACAACAATAATAAATGTTAGT
CATAGAGAGGGGAATTCACA

Downstream 100 bases:

>100_bases
GATAGTTAATAACTGATTTAGAAGCTGGGAACTGCTTCCTGCTTCTAAATTTTATATAAATTCCTTTTCTAGTTCTTGTT
GTATATCCCCAAAATATATC

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2 [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPSPTKIWKGSYEDCKDADIVCIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDTGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
DYFDVDPRNVHAYIIGEHGDTELPVWSHATIGVQKIEKIMSDNKEKYSQKDLEDIFVNVRDAAYHIIERKGATYYGIGMS
LLRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAIINRQGVREIVELELNEEEKAKFAHSVKVLKETMAPVL

Sequences:

>Translated_315_residues
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPSPTKIWKGSYEDCKDADIVCIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDTGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
DYFDVDPRNVHAYIIGEHGDTELPVWSHATIGVQKIEKIMSDNKEKYSQKDLEDIFVNVRDAAYHIIERKGATYYGIGMS
LLRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAIINRQGVREIVELELNEEEKAKFAHSVKVLKETMAPVL
>Mature_315_residues
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPSPTKIWKGSYEDCKDADIVCIT
AGLPQKPGETRLDLVEKNAKIFKQIVRSIMDTGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
DYFDVDPRNVHAYIIGEHGDTELPVWSHATIGVQKIEKIMSDNKEKYSQKDLEDIFVNVRDAAYHIIERKGATYYGIGMS
LLRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAIINRQGVREIVELELNEEEKAKFAHSVKVLKETMAPVL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=40.9836065573771, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=40.9836065573771, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=38.0327868852459, Blast_Score=237, Evalue=9e-63,
Organism=Homo sapiens, GI5031857, Length=303, Percent_Identity=39.2739273927393, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI260099723, Length=303, Percent_Identity=39.2739273927393, Blast_Score=236, Evalue=1e-62,
Organism=Homo sapiens, GI291575128, Length=306, Percent_Identity=38.2352941176471, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI4557032, Length=306, Percent_Identity=38.2352941176471, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI9257228, Length=306, Percent_Identity=41.5032679738562, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI4504973, Length=306, Percent_Identity=41.5032679738562, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI260099727, Length=211, Percent_Identity=43.1279620853081, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI260099725, Length=221, Percent_Identity=42.0814479638009, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI207028494, Length=204, Percent_Identity=37.7450980392157, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI103472011, Length=314, Percent_Identity=26.4331210191083, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI103472015, Length=203, Percent_Identity=28.0788177339901, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI21735621, Length=293, Percent_Identity=25.2559726962457, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789632, Length=298, Percent_Identity=28.8590604026846, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535107, Length=291, Percent_Identity=40.2061855670103, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17554310, Length=306, Percent_Identity=27.1241830065359, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322765, Length=174, Percent_Identity=32.183908045977, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136226, Length=313, Percent_Identity=37.6996805111821, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=27.6872964169381, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=27.741935483871, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24663599, Length=325, Percent_Identity=25.2307692307692, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34916; Mature: 34916

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPSP
CCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCC
TKIWKGSYEDCKDADIVCITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDTGFDGIFLI
CHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGDYFDVDPRNVHAYIIGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHCCEECCCCCCEEEEEEECCCC
TELPVWSHATIGVQKIEKIMSDNKEKYSQKDLEDIFVNVRDAAYHIIERKGATYYGIGMS
CCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LLRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAIINRQGVREIVELELNEEEKAKF
HHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHHHCHHHHHHHHHHCCCCHHHHHH
AHSVKVLKETMAPVL
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPSP
CCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCC
TKIWKGSYEDCKDADIVCITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDTGFDGIFLI
CHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGDYFDVDPRNVHAYIIGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHCCEECCCCCCEEEEEEECCCC
TELPVWSHATIGVQKIEKIMSDNKEKYSQKDLEDIFVNVRDAAYHIIERKGATYYGIGMS
CCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LLRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAIINRQGVREIVELELNEEEKAKF
HHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHHHCHHHHHHHHHHCCCCHHHHHH
AHSVKVLKETMAPVL
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]