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The map label for this gene is glgB

Identifier: 152977187

GI number: 152977187

Start: 3565735

End: 3567672

Strand: Reverse

Name: glgB

Synonym: Bcer98_3504

Alternate gene names: 152977187

Gene position: 3567672-3565735 (Counterclockwise)

Preceding gene: 152977188

Following gene: 152977186

Centisome position: 87.29

GC content: 35.04

Gene sequence:

>1938_bases
TTGCGTGCCATACATTGTTTAGAAGAAAGGTTAGAACAATTTCATACAGAGAAATGTTATGAGAGCTATCAAATATTTGG
AGCACATCTTGCAGTAGAAAATGGAGTGCATGGTGTACGTTTTACTGTATGGGCTCCTCGTGCTAAAAATCTAAGTGTTG
TTGGAGATTTTAATGAATGGAATGAAAAGCAACATAGGATGCAAAAAGTGACAGAAGCGGGGATTTGGTCGCTATTTATT
CCTCAAATAGAAGAAAAAGAAATATATAAATATGCTATTGAGACCATCAATGGCGAAGTGATTTTGAAAGCAGATCCTTA
TGCAACATATGCAGAAGTAAGACCAAATACGGCATCTGTTATTTTGGATATAGAGGGATACGAATGGAATGATAAAAATT
GGTTTCGTAAAAAGAAAAAAAAGTCAGTTTATAAAGAAGCAATGGCAATTTATGAATTGCATTTTGGATCATGGAAAAAG
AAAGAAGATGGTTCTTTATACTCATATCGTGAAATGGCAGAGGAATTGATTCCTTATATGGCAAATCATCATTTTACTCA
TATTGAGATTATGCCGCTTGTTGAGCATCCATATGATCGGTCATGGGGATATCAAGGGACGGGATACTATGCAGTGACAA
GTCGTTTCGGCACGCCGCATGATTTTATGTATTTTGTCGATGAATGTCATAAATATGGAATCGGTGTCATTTTAGATTGG
GTACCAGGGCATTTTTGCAAAGATGCTCATGGCTTATATTTATTCGATGGAACACCAACCTATGAATATAGAGATTTAGA
TGTACAAGAAAATCGTGTTTGGGGAACGGCAAACTTTGATTTAGGAAAGCGAGAGGTAAGGAATTTTTTAATTTCAAATG
CACTATTTTGGATGAAATATTATCATATTGATGGTTTTCGTGTTGATGCAGTTGCGAATATGCTGTATTGGGAGAAAGAG
GGGAAAGCACAGAGTAATGAATATGCAGTTTCTTTTTTGCGTGAATTAAATGAAGCAGTATTTGCAGAAGATGGCGAATT
TCTAATGACTGCTGAAGATTCAACAGCTTGGCCTCTTGTAACTGCTCCAACTTATGAAGGGGGATTAGGATTTAATTATA
AGTGGAATATGGGTTGGATGAATGATGTTTTGAAATATATGGAGTGTGCTCCAGAGTATCGAAAATATATTCATGAAAAA
ATGACATTTTCTTTATTATATGCTTACTCAGAAAATTTTATTTTACCTCTTTCACATGATGAGGTCGTTCATGGAAAGAA
ATCATTGCTGAACAAAATGCCAGGAAACTATTGGGAGAAGTTTGCACAGCTTCGCTTATTATATGGATATTTCTTTACAC
ATCCAGGAAAGAAATTATTGTTTATGGGAGGCGAATTTGGACAATTTGATGAGTGGAAAGATCTTGAAGATTTAGATTGG
AATTTACATGAATTTGAAATGCATCGTCATATGCATGATTACTTTAAAGAACTCATAGCATTGTATAAGCGTTCTAAACC
ACTTTGGCAGCTCGATTATTCTCATGAAGGATTCCAATGGATTGATGCTGATAATAAAGAGCAAAGTATTTTCTCGTTTA
TTCGTAAAGGAGATAGAGAAGATGATGTTCTGATTGTGATATGCAATTTTACTAGCATTGTGTATGAAAAGTATAAGGTT
GGCGTACCAGAGTTTCAATATTATAACGAGATTTTAAATAGTGACGCGATAGCATATGGTGGTTCAGGAAGAATAAATAA
AAAGCGTTTAAAAAGCATTTCACAGCCATATCATAATCAAACTGCTCATGTAGAAATTACAATTCCACCATTTGGCGTAT
CTATTTTAAGACCAGTGAAAATGAGAAAGGGGAGCAAAAAACAAGATGGTAAAAAAGCAGAATTGCGTAGCAATGCTACT
AGCCGGAGGAAAAGGTAG

Upstream 100 bases:

>100_bases
AAAAAACGGATATTTATTTCAAAAGGAACAAGGATATTTCATCCCTATAGCGAATGTTGAATGTATTGAAAAATTCTGTC
ATCTTCGTGAGGTGAACATA

Downstream 100 bases:

>100_bases
TCGCTTAAGTGCATTAACAAAAAACTTAGCGAAGCCAGCTGTTCCTTTTGGTGGTAAGTATAGAATTATTGACTTTACAT
TAAGTAATTGCTCCAACTCT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MRAIHCLEERLEQFHTEKCYESYQIFGAHLAVENGVHGVRFTVWAPRAKNLSVVGDFNEWNEKQHRMQKVTEAGIWSLFI
PQIEEKEIYKYAIETINGEVILKADPYATYAEVRPNTASVILDIEGYEWNDKNWFRKKKKKSVYKEAMAIYELHFGSWKK
KEDGSLYSYREMAEELIPYMANHHFTHIEIMPLVEHPYDRSWGYQGTGYYAVTSRFGTPHDFMYFVDECHKYGIGVILDW
VPGHFCKDAHGLYLFDGTPTYEYRDLDVQENRVWGTANFDLGKREVRNFLISNALFWMKYYHIDGFRVDAVANMLYWEKE
GKAQSNEYAVSFLRELNEAVFAEDGEFLMTAEDSTAWPLVTAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHEK
MTFSLLYAYSENFILPLSHDEVVHGKKSLLNKMPGNYWEKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
NLHEFEMHRHMHDYFKELIALYKRSKPLWQLDYSHEGFQWIDADNKEQSIFSFIRKGDREDDVLIVICNFTSIVYEKYKV
GVPEFQYYNEILNSDAIAYGGSGRINKKRLKSISQPYHNQTAHVEITIPPFGVSILRPVKMRKGSKKQDGKKAELRSNAT
SRRKR

Sequences:

>Translated_645_residues
MRAIHCLEERLEQFHTEKCYESYQIFGAHLAVENGVHGVRFTVWAPRAKNLSVVGDFNEWNEKQHRMQKVTEAGIWSLFI
PQIEEKEIYKYAIETINGEVILKADPYATYAEVRPNTASVILDIEGYEWNDKNWFRKKKKKSVYKEAMAIYELHFGSWKK
KEDGSLYSYREMAEELIPYMANHHFTHIEIMPLVEHPYDRSWGYQGTGYYAVTSRFGTPHDFMYFVDECHKYGIGVILDW
VPGHFCKDAHGLYLFDGTPTYEYRDLDVQENRVWGTANFDLGKREVRNFLISNALFWMKYYHIDGFRVDAVANMLYWEKE
GKAQSNEYAVSFLRELNEAVFAEDGEFLMTAEDSTAWPLVTAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHEK
MTFSLLYAYSENFILPLSHDEVVHGKKSLLNKMPGNYWEKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
NLHEFEMHRHMHDYFKELIALYKRSKPLWQLDYSHEGFQWIDADNKEQSIFSFIRKGDREDDVLIVICNFTSIVYEKYKV
GVPEFQYYNEILNSDAIAYGGSGRINKKRLKSISQPYHNQTAHVEITIPPFGVSILRPVKMRKGSKKQDGKKAELRSNAT
SRRKR
>Mature_645_residues
MRAIHCLEERLEQFHTEKCYESYQIFGAHLAVENGVHGVRFTVWAPRAKNLSVVGDFNEWNEKQHRMQKVTEAGIWSLFI
PQIEEKEIYKYAIETINGEVILKADPYATYAEVRPNTASVILDIEGYEWNDKNWFRKKKKKSVYKEAMAIYELHFGSWKK
KEDGSLYSYREMAEELIPYMANHHFTHIEIMPLVEHPYDRSWGYQGTGYYAVTSRFGTPHDFMYFVDECHKYGIGVILDW
VPGHFCKDAHGLYLFDGTPTYEYRDLDVQENRVWGTANFDLGKREVRNFLISNALFWMKYYHIDGFRVDAVANMLYWEKE
GKAQSNEYAVSFLRELNEAVFAEDGEFLMTAEDSTAWPLVTAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHEK
MTFSLLYAYSENFILPLSHDEVVHGKKSLLNKMPGNYWEKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
NLHEFEMHRHMHDYFKELIALYKRSKPLWQLDYSHEGFQWIDADNKEQSIFSFIRKGDREDDVLIVICNFTSIVYEKYKV
GVPEFQYYNEILNSDAIAYGGSGRINKKRLKSISQPYHNQTAHVEITIPPFGVSILRPVKMRKGSKKQDGKKAELRSNAT
SRRKR

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=630, Percent_Identity=26.984126984127, Blast_Score=187, Evalue=3e-47,
Organism=Escherichia coli, GI1789839, Length=604, Percent_Identity=44.5364238410596, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17554896, Length=644, Percent_Identity=25.776397515528, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI32564391, Length=603, Percent_Identity=24.7097844112769, Blast_Score=158, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320826, Length=651, Percent_Identity=26.1136712749616, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI28573410, Length=682, Percent_Identity=26.6862170087977, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_BACCN (A7GUA1)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376704.1
- ProteinModelPortal:   A7GUA1
- SMR:   A7GUA1
- STRING:   A7GUA1
- EnsemblBacteria:   EBBACT00000037882
- GeneID:   5347533
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_3504
- eggNOG:   COG0296
- GeneTree:   EBGT00070000031950
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK05402
- BioCyc:   BCER315749:BCER98_3504-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 76147; Mature: 76147

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: ACT_SITE 204-204 ACT_SITE 239-239 ACT_SITE 244-244 ACT_SITE 307-307 ACT_SITE 309-309 ACT_SITE 352-352 ACT_SITE 419-419 ACT_SITE 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAIHCLEERLEQFHTEKCYESYQIFGAHLAVENGVHGVRFTVWAPRAKNLSVVGDFNEW
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCEEEEECCHHH
NEKQHRMQKVTEAGIWSLFIPQIEEKEIYKYAIETINGEVILKADPYATYAEVRPNTASV
HHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEE
ILDIEGYEWNDKNWFRKKKKKSVYKEAMAIYELHFGSWKKKEDGSLYSYREMAEELIPYM
EEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ANHHFTHIEIMPLVEHPYDRSWGYQGTGYYAVTSRFGTPHDFMYFVDECHKYGIGVILDW
HCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEC
VPGHFCKDAHGLYLFDGTPTYEYRDLDVQENRVWGTANFDLGKREVRNFLISNALFWMKY
CCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEHHE
YHIDGFRVDAVANMLYWEKEGKAQSNEYAVSFLRELNEAVFAEDGEFLMTAEDSTAWPLV
EECCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
TAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHEKMTFSLLYAYSENFILPLSHD
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCEEEECCCC
EVVHGKKSLLNKMPGNYWEKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
HHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
NLHEFEMHRHMHDYFKELIALYKRSKPLWQLDYSHEGFQWIDADNKEQSIFSFIRKGDRE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHCCCCC
DDVLIVICNFTSIVYEKYKVGVPEFQYYNEILNSDAIAYGGSGRINKKRLKSISQPYHNQ
CCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCC
TAHVEITIPPFGVSILRPVKMRKGSKKQDGKKAELRSNATSRRKR
CEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCC
>Mature Secondary Structure
MRAIHCLEERLEQFHTEKCYESYQIFGAHLAVENGVHGVRFTVWAPRAKNLSVVGDFNEW
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCEEEEECCHHH
NEKQHRMQKVTEAGIWSLFIPQIEEKEIYKYAIETINGEVILKADPYATYAEVRPNTASV
HHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEE
ILDIEGYEWNDKNWFRKKKKKSVYKEAMAIYELHFGSWKKKEDGSLYSYREMAEELIPYM
EEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ANHHFTHIEIMPLVEHPYDRSWGYQGTGYYAVTSRFGTPHDFMYFVDECHKYGIGVILDW
HCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEC
VPGHFCKDAHGLYLFDGTPTYEYRDLDVQENRVWGTANFDLGKREVRNFLISNALFWMKY
CCCCCCCCCCCEEEECCCCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEHHE
YHIDGFRVDAVANMLYWEKEGKAQSNEYAVSFLRELNEAVFAEDGEFLMTAEDSTAWPLV
EECCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEE
TAPTYEGGLGFNYKWNMGWMNDVLKYMECAPEYRKYIHEKMTFSLLYAYSENFILPLSHD
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCEEEECCCC
EVVHGKKSLLNKMPGNYWEKFAQLRLLYGYFFTHPGKKLLFMGGEFGQFDEWKDLEDLDW
HHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCC
NLHEFEMHRHMHDYFKELIALYKRSKPLWQLDYSHEGFQWIDADNKEQSIFSFIRKGDRE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHCCCCC
DDVLIVICNFTSIVYEKYKVGVPEFQYYNEILNSDAIAYGGSGRINKKRLKSISQPYHNQ
CCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCC
TAHVEITIPPFGVSILRPVKMRKGSKKQDGKKAELRSNATSRRKR
CEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA