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The map label for this gene is menF [H]

Identifier: 152977178

GI number: 152977178

Start: 3553681

End: 3555075

Strand: Reverse

Name: menF [H]

Synonym: Bcer98_3494

Alternate gene names: 152977178

Gene position: 3555075-3553681 (Counterclockwise)

Preceding gene: 152977181

Following gene: 152977177

Centisome position: 86.98

GC content: 39.28

Gene sequence:

>1395_bases
GTGATTCAAACGAAACAAAAAGACTTGCAAGAAGTACTCACTACAGCGATTGAAAAGAGTACAAATGAACAGACATTGGT
TAGTTTTGTAAAACAAATAGATTGGATAGATCCGTTGCTGTTTTATGCGGCAGGAAAAAGGATTGCATCCGAGCATAGAT
GTTATTTTGCAGATCCAGCAGAGCGCGTTATATTTGCGGGGATTGGTTCTGTTTTCAACATAGTAAACTCTTCAAATGCA
CGCTTTCAAGATGCTCGTCAGCAGTGGAACAGAGTACAAGAGAAGGCGGTTATTCAAAGAGAAGCGTATGAATTTGGGAC
AGGTCCGCTTTTATTTGGCGGTTTTTCATTTGATCCAGAAAAGGAACAAACGGAGCTTTGGAAGGAATTTAAAGATACAA
CATTTTCATTGCCAGCCTTTTTATTAACAGTAAAAAACGAAAAAGCATGGCTTACGATAAATACTTTTGTTTCGGCTAAA
GATTCTGCGGATAAGAAATATGAAGAAATTTCTTCTTTAGAGGAAAGAATTTTACAAGAAAGTGAATATCCGCTTGAAGA
ATCGCGATTAACGGTTACTTCTAAAGTGGAAGTAGACCCGCAAGGATGGATGCATGCAATTGAAAAAGTACAAGAGGAAA
TGAGAAAGGGAAACATACAGAAAGTGGTATTAGCGCGAGAACTTAAATTAGAAATAGACCAACAAATTGATTCTGCTCGC
GTGCTAGAAGCACTTCGTATTGGACAGCCGGATTGCTATGTATTTTCTTTTGATTATAAAGGAGCGTGCTTCTTAGGAGC
GACACCGGAACGATTAATCCGTAAAGAAGGGAATAAGTTCACTTCTATGTGCCTTGCTGGATCAATTGGGCAGGGGAATT
CTATAGAAGAGAGTCAAAAAAATGGCGAAATGCTCCTTCATGATGAAAAGAATTTAGTAGAGCATGGTTATGTAGTAAAT
ATGATCCGTAGTGTATTACAGGAACATTGCGAATTTGTTAATATTCCAGAGAGTCCAGGATTATTAACAACGAAAAATTT
AATTCATTTATATACACCTGTTGAAGCGGAGGGGGATGCTTCCCTTTTAACATTGGTAGAAGAATTGCATCCAACGCCAG
CTCTTGGTGGCACACCTCGTCACGTAGCGATGAAATTAATTCGTGATGTTGAATTATTAGATAGAGGATTGTACGGGGCA
CCAATCGGTTGGATGGATGAGCAAGGGAACGGAGAATTTGCAGTGGCATTGCGCTGTGGATTATTAAATGGTGAAAAGGC
ATCCTTATTTGCGGGCTGTGGCATTGTAATGGATTCTGTCCCACAGCTTGAATATGAAGAGACAAGTTTGAAGTTTAGAC
CGATGCTTGGTGCTTTGGAGGAATTGATGAAATGA

Upstream 100 bases:

>100_bases
ATTTCTCAGAAATGCTAATCGTTGACATGACTTTCGTTTGAGGTGTATCTTAAAATCAAGCGTATTTATGACTATTTTTC
GCCCATAGGGGGGAGACTTT

Downstream 100 bases:

>100_bases
ATAATCATATAGAAGCATTATCATATTATTTAGGCGCGTTTGTTGACGAACTAGCGCGTCTAAATGTATGTGATGTTGTC
ATTAGTCCAGGCTCGCGATC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIQTKQKDLQEVLTTAIEKSTNEQTLVSFVKQIDWIDPLLFYAAGKRIASEHRCYFADPAERVIFAGIGSVFNIVNSSNA
RFQDARQQWNRVQEKAVIQREAYEFGTGPLLFGGFSFDPEKEQTELWKEFKDTTFSLPAFLLTVKNEKAWLTINTFVSAK
DSADKKYEEISSLEERILQESEYPLEESRLTVTSKVEVDPQGWMHAIEKVQEEMRKGNIQKVVLARELKLEIDQQIDSAR
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEGNKFTSMCLAGSIGQGNSIEESQKNGEMLLHDEKNLVEHGYVVN
MIRSVLQEHCEFVNIPESPGLLTTKNLIHLYTPVEAEGDASLLTLVEELHPTPALGGTPRHVAMKLIRDVELLDRGLYGA
PIGWMDEQGNGEFAVALRCGLLNGEKASLFAGCGIVMDSVPQLEYEETSLKFRPMLGALEELMK

Sequences:

>Translated_464_residues
MIQTKQKDLQEVLTTAIEKSTNEQTLVSFVKQIDWIDPLLFYAAGKRIASEHRCYFADPAERVIFAGIGSVFNIVNSSNA
RFQDARQQWNRVQEKAVIQREAYEFGTGPLLFGGFSFDPEKEQTELWKEFKDTTFSLPAFLLTVKNEKAWLTINTFVSAK
DSADKKYEEISSLEERILQESEYPLEESRLTVTSKVEVDPQGWMHAIEKVQEEMRKGNIQKVVLARELKLEIDQQIDSAR
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEGNKFTSMCLAGSIGQGNSIEESQKNGEMLLHDEKNLVEHGYVVN
MIRSVLQEHCEFVNIPESPGLLTTKNLIHLYTPVEAEGDASLLTLVEELHPTPALGGTPRHVAMKLIRDVELLDRGLYGA
PIGWMDEQGNGEFAVALRCGLLNGEKASLFAGCGIVMDSVPQLEYEETSLKFRPMLGALEELMK
>Mature_464_residues
MIQTKQKDLQEVLTTAIEKSTNEQTLVSFVKQIDWIDPLLFYAAGKRIASEHRCYFADPAERVIFAGIGSVFNIVNSSNA
RFQDARQQWNRVQEKAVIQREAYEFGTGPLLFGGFSFDPEKEQTELWKEFKDTTFSLPAFLLTVKNEKAWLTINTFVSAK
DSADKKYEEISSLEERILQESEYPLEESRLTVTSKVEVDPQGWMHAIEKVQEEMRKGNIQKVVLARELKLEIDQQIDSAR
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEGNKFTSMCLAGSIGQGNSIEESQKNGEMLLHDEKNLVEHGYVVN
MIRSVLQEHCEFVNIPESPGLLTTKNLIHLYTPVEAEGDASLLTLVEELHPTPALGGTPRHVAMKLIRDVELLDRGLYGA
PIGWMDEQGNGEFAVALRCGLLNGEKASLFAGCGIVMDSVPQLEYEETSLKFRPMLGALEELMK

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=241, Percent_Identity=41.0788381742739, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87082077, Length=295, Percent_Identity=30.8474576271186, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787518, Length=379, Percent_Identity=24.802110817942, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=266, Percent_Identity=24.812030075188, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324361, Length=300, Percent_Identity=22, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52300; Mature: 52300

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTKQKDLQEVLTTAIEKSTNEQTLVSFVKQIDWIDPLLFYAAGKRIASEHRCYFADPA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHH
ERVIFAGIGSVFNIVNSSNARFQDARQQWNRVQEKAVIQREAYEFGTGPLLFGGFSFDPE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCC
KEQTELWKEFKDTTFSLPAFLLTVKNEKAWLTINTFVSAKDSADKKYEEISSLEERILQE
HHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHC
SEYPLEESRLTVTSKVEVDPQGWMHAIEKVQEEMRKGNIQKVVLARELKLEIDQQIDSAR
CCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEGNKFTSMCLAGSIGQGNSIEESQK
HHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHC
NGEMLLHDEKNLVEHGYVVNMIRSVLQEHCEFVNIPESPGLLTTKNLIHLYTPVEAEGDA
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCEEEEECCCCCCCCH
SLLTLVEELHPTPALGGTPRHVAMKLIRDVELLDRGLYGAPIGWMDEQGNGEFAVALRCG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
LLNGEKASLFAGCGIVMDSVPQLEYEETSLKFRPMLGALEELMK
CCCCCCHHHHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MIQTKQKDLQEVLTTAIEKSTNEQTLVSFVKQIDWIDPLLFYAAGKRIASEHRCYFADPA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHH
ERVIFAGIGSVFNIVNSSNARFQDARQQWNRVQEKAVIQREAYEFGTGPLLFGGFSFDPE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCC
KEQTELWKEFKDTTFSLPAFLLTVKNEKAWLTINTFVSAKDSADKKYEEISSLEERILQE
HHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHC
SEYPLEESRLTVTSKVEVDPQGWMHAIEKVQEEMRKGNIQKVVLARELKLEIDQQIDSAR
CCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEGNKFTSMCLAGSIGQGNSIEESQK
HHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHC
NGEMLLHDEKNLVEHGYVVNMIRSVLQEHCEFVNIPESPGLLTTKNLIHLYTPVEAEGDA
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCEEEEECCCCCCCCH
SLLTLVEELHPTPALGGTPRHVAMKLIRDVELLDRGLYGAPIGWMDEQGNGEFAVALRCG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
LLNGEKASLFAGCGIVMDSVPQLEYEETSLKFRPMLGALEELMK
CCCCCCHHHHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]