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The map label for this gene is menD

Identifier: 152977177

GI number: 152977177

Start: 3551930

End: 3553684

Strand: Reverse

Name: menD

Synonym: Bcer98_3493

Alternate gene names: 152977177

Gene position: 3553684-3551930 (Counterclockwise)

Preceding gene: 152977178

Following gene: 152977176

Centisome position: 86.95

GC content: 39.2

Gene sequence:

>1755_bases
ATGAATAATCATATAGAAGCATTATCATATTATTTAGGCGCGTTTGTTGACGAACTAGCGCGTCTAAATGTATGTGATGT
TGTCATTAGTCCAGGCTCGCGATCAACACCGCTAGCCTTATTAATGGAGCAGCATGAACAAATAAAAACATATTTACACG
TAGATGAACGATCCGCGGCCTTTTTCGCGCTCGGTATGGCGAAAGCGAAAAAACAGCCTGTGGCGATATTATGTACGTCG
GGAACAGCCGCAGCAAATTATTATCCAGCTGTTTGTGAAGCGTATCATGCAAGAGTACCCCTACTCGTTTTAACTGCAGA
TAGACCGCATGAATTGAGAGATGTAGGAGCACCGCAGGCAATGAATCAATTCAATTTATACGGCTCATTTGTGAAACAAT
TTATGGAAATGGCACTTCCAGAAGCGAGAGAGCCAATGTATCAATATGTTCGCATGGCAGCTGGACGTGCTGTGGCAAGT
GCATCTTTTGCACCAATGGGACCTGTCCATATGAACTTCCCACTTCGCGAACCACTTATTCCAGACTTTTCGTTAGATGG
ATTGTGGGAACAAGGGTGCGGAGAATATACGAATAGGGTTCAACAAGGAAGCATGACGCTAACGAGTGAATATATCCGTT
CTCTTATAAAGCGCCTTTCACGTCTAGAAAAGGGGCTTATTGTTTGTGGAGACGATAGTCACCTAGAGCTTGTAGAAGTG
ATTGCGGAGTTTGCGGAGAAAACAGGATACCCAGTTTTAGCGGACCCACTTTCAAATCTTCGGACTGGAAGCCATAATCA
AACAATGATTATCGATTGCTATGATACCTTTTTGCGAAATGAATTATTAAAAGATACGTGGAAGCCAGATATCATCATCC
GTTTTGGTGGTATGCCTGTATCAAAAGCATTAACGCAATATATAAAAAAACAAGAGAGTGCTGTTCATATCATTGTAGAT
GAATCTGGAAAGTGGCGAGACCCAGCTTTAATGACAACAGAAGTTGTTTCTGCAAGCGATGTGGCATTTTGCAAAGCGAT
GACAGAACATATGCAAAAACGAGAACAGAATGACTGGTTCAAAAAATGGAAACATATAAACGATAAAACAAAGGAAACAC
TTCGTGAAGTTGAAGCATATGATACTGCTTTTGAAGGAAAAGTGATTACAGATATTGTTCGTATATTACCAGAAGGTGCG
ACATTATTTGTAAGTAACAGTATGCCAATTCGTGATGCAGATACATTTTTATTTACAAACGAGAAAAAAATACATGTGAT
GGCCAATCGTGGTGTGAATGGTATTGATGGAATTATTTCCACTGCATTAGGAGCAAGCACTGTTTGTGAACCACTTGTAT
TAGTAATTGGTGATTTATCATTTTATCATGATTTAAATGGTCTTTTAGCAGCGAAATTACATGATTTAAATATAACTATT
GTTGTTGTGAATAATGATGGTGGAGGTATTTTTTCATTCTTACCACAATATGAGTCAAAAGAGCATTTTGAATCGCTATT
TGGAACACCGCTTGGTCTTGATTATGAGCATGTTGTAAAGATGTACGGTGGTTCATTTGTTCGCGTAAGTGGCTGGGAAG
CATTTCGTGAGGAAGTACAAAAAGGAATAATAGAAAGAGGATTACATGTTGTGGAAATTTGTACGAATCGAGAAGAAAAT
GTACAGTTGCATCGTAAATTGTGGGCAAAATCCGTAATAGAAATCAAAGACATGTTGCAAGGTGACACGGAATGA

Upstream 100 bases:

>100_bases
CGGGCTGTGGCATTGTAATGGATTCTGTCCCACAGCTTGAATATGAAGAGACAAGTTTGAAGTTTAGACCGATGCTTGGT
GCTTTGGAGGAATTGATGAA

Downstream 100 bases:

>100_bases
AAGTAACACTAGAAGGTGTGGCGTATGAATATGAAGTGGCTGGAAGTGGGGAGGCATTATTGCTCCTTCATGGTTTTACA
GGAAGCATGGAAACATGGCG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MNNHIEALSYYLGAFVDELARLNVCDVVISPGSRSTPLALLMEQHEQIKTYLHVDERSAAFFALGMAKAKKQPVAILCTS
GTAAANYYPAVCEAYHARVPLLVLTADRPHELRDVGAPQAMNQFNLYGSFVKQFMEMALPEAREPMYQYVRMAAGRAVAS
ASFAPMGPVHMNFPLREPLIPDFSLDGLWEQGCGEYTNRVQQGSMTLTSEYIRSLIKRLSRLEKGLIVCGDDSHLELVEV
IAEFAEKTGYPVLADPLSNLRTGSHNQTMIIDCYDTFLRNELLKDTWKPDIIIRFGGMPVSKALTQYIKKQESAVHIIVD
ESGKWRDPALMTTEVVSASDVAFCKAMTEHMQKREQNDWFKKWKHINDKTKETLREVEAYDTAFEGKVITDIVRILPEGA
TLFVSNSMPIRDADTFLFTNEKKIHVMANRGVNGIDGIISTALGASTVCEPLVLVIGDLSFYHDLNGLLAAKLHDLNITI
VVVNNDGGGIFSFLPQYESKEHFESLFGTPLGLDYEHVVKMYGGSFVRVSGWEAFREEVQKGIIERGLHVVEICTNREEN
VQLHRKLWAKSVIEIKDMLQGDTE

Sequences:

>Translated_584_residues
MNNHIEALSYYLGAFVDELARLNVCDVVISPGSRSTPLALLMEQHEQIKTYLHVDERSAAFFALGMAKAKKQPVAILCTS
GTAAANYYPAVCEAYHARVPLLVLTADRPHELRDVGAPQAMNQFNLYGSFVKQFMEMALPEAREPMYQYVRMAAGRAVAS
ASFAPMGPVHMNFPLREPLIPDFSLDGLWEQGCGEYTNRVQQGSMTLTSEYIRSLIKRLSRLEKGLIVCGDDSHLELVEV
IAEFAEKTGYPVLADPLSNLRTGSHNQTMIIDCYDTFLRNELLKDTWKPDIIIRFGGMPVSKALTQYIKKQESAVHIIVD
ESGKWRDPALMTTEVVSASDVAFCKAMTEHMQKREQNDWFKKWKHINDKTKETLREVEAYDTAFEGKVITDIVRILPEGA
TLFVSNSMPIRDADTFLFTNEKKIHVMANRGVNGIDGIISTALGASTVCEPLVLVIGDLSFYHDLNGLLAAKLHDLNITI
VVVNNDGGGIFSFLPQYESKEHFESLFGTPLGLDYEHVVKMYGGSFVRVSGWEAFREEVQKGIIERGLHVVEICTNREEN
VQLHRKLWAKSVIEIKDMLQGDTE
>Mature_584_residues
MNNHIEALSYYLGAFVDELARLNVCDVVISPGSRSTPLALLMEQHEQIKTYLHVDERSAAFFALGMAKAKKQPVAILCTS
GTAAANYYPAVCEAYHARVPLLVLTADRPHELRDVGAPQAMNQFNLYGSFVKQFMEMALPEAREPMYQYVRMAAGRAVAS
ASFAPMGPVHMNFPLREPLIPDFSLDGLWEQGCGEYTNRVQQGSMTLTSEYIRSLIKRLSRLEKGLIVCGDDSHLELVEV
IAEFAEKTGYPVLADPLSNLRTGSHNQTMIIDCYDTFLRNELLKDTWKPDIIIRFGGMPVSKALTQYIKKQESAVHIIVD
ESGKWRDPALMTTEVVSASDVAFCKAMTEHMQKREQNDWFKKWKHINDKTKETLREVEAYDTAFEGKVITDIVRILPEGA
TLFVSNSMPIRDADTFLFTNEKKIHVMANRGVNGIDGIISTALGASTVCEPLVLVIGDLSFYHDLNGLLAAKLHDLNITI
VVVNNDGGGIFSFLPQYESKEHFESLFGTPLGLDYEHVVKMYGGSFVRVSGWEAFREEVQKGIIERGLHVVEICTNREEN
VQLHRKLWAKSVIEIKDMLQGDTE

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=567, Percent_Identity=30.8641975308642, Blast_Score=218, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_BACCN (A7GU91)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376694.1
- ProteinModelPortal:   A7GU91
- STRING:   A7GU91
- EnsemblBacteria:   EBBACT00000038378
- GeneID:   5346756
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_3493
- eggNOG:   COG1165
- GeneTree:   EBGT00050000000626
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   BCER315749:BCER98_3493-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 65510; Mature: 65510

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHIEALSYYLGAFVDELARLNVCDVVISPGSRSTPLALLMEQHEQIKTYLHVDERSAA
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCHH
FFALGMAKAKKQPVAILCTSGTAAANYYPAVCEAYHARVPLLVLTADRPHELRDVGAPQA
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQFNLYGSFVKQFMEMALPEAREPMYQYVRMAAGRAVASASFAPMGPVHMNFPLREPLI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEECCCCCCCCC
PDFSLDGLWEQGCGEYTNRVQQGSMTLTSEYIRSLIKRLSRLEKGLIVCGDDSHLELVEV
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
IAEFAEKTGYPVLADPLSNLRTGSHNQTMIIDCYDTFLRNELLKDTWKPDIIIRFGGMPV
HHHHHHHCCCCEEECCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCH
SKALTQYIKKQESAVHIIVDESGKWRDPALMTTEVVSASDVAFCKAMTEHMQKREQNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KKWKHINDKTKETLREVEAYDTAFEGKVITDIVRILPEGATLFVSNSMPIRDADTFLFTN
HHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEC
EKKIHVMANRGVNGIDGIISTALGASTVCEPLVLVIGDLSFYHDLNGLLAAKLHDLNITI
CCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEE
VVVNNDGGGIFSFLPQYESKEHFESLFGTPLGLDYEHVVKMYGGSFVRVSGWEAFREEVQ
EEEECCCCCHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHH
KGIIERGLHVVEICTNREENVQLHRKLWAKSVIEIKDMLQGDTE
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNHIEALSYYLGAFVDELARLNVCDVVISPGSRSTPLALLMEQHEQIKTYLHVDERSAA
CCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCHH
FFALGMAKAKKQPVAILCTSGTAAANYYPAVCEAYHARVPLLVLTADRPHELRDVGAPQA
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHH
MNQFNLYGSFVKQFMEMALPEAREPMYQYVRMAAGRAVASASFAPMGPVHMNFPLREPLI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEECCCCCCCCC
PDFSLDGLWEQGCGEYTNRVQQGSMTLTSEYIRSLIKRLSRLEKGLIVCGDDSHLELVEV
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHH
IAEFAEKTGYPVLADPLSNLRTGSHNQTMIIDCYDTFLRNELLKDTWKPDIIIRFGGMPV
HHHHHHHCCCCEEECCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCH
SKALTQYIKKQESAVHIIVDESGKWRDPALMTTEVVSASDVAFCKAMTEHMQKREQNDWF
HHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
KKWKHINDKTKETLREVEAYDTAFEGKVITDIVRILPEGATLFVSNSMPIRDADTFLFTN
HHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEC
EKKIHVMANRGVNGIDGIISTALGASTVCEPLVLVIGDLSFYHDLNGLLAAKLHDLNITI
CCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECEEEE
VVVNNDGGGIFSFLPQYESKEHFESLFGTPLGLDYEHVVKMYGGSFVRVSGWEAFREEVQ
EEEECCCCCHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHH
KGIIERGLHVVEICTNREENVQLHRKLWAKSVIEIKDMLQGDTE
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA