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The map label for this gene is moaA

Identifier: 152977087

GI number: 152977087

Start: 3460955

End: 3461968

Strand: Reverse

Name: moaA

Synonym: Bcer98_3397

Alternate gene names: 152977087

Gene position: 3461968-3460955 (Counterclockwise)

Preceding gene: 152977093

Following gene: 152977086

Centisome position: 84.71

GC content: 37.57

Gene sequence:

>1014_bases
ATGCACGAGAAGGTGAGGGATTCTTTCGGGCGCCCGCTTCAAGATTTACGGATTTCAGTTATAGATCGCTGTAATTTTAG
ATGCACGTACTGTATGCCGGCTGAAGTATTTGGACCAGATTATGCTTTTTTACAAGAAGAATTTTTACTTACATTTGATG
AGATTGAAAGATTAGCAAAATTATTTGCTGGCATAGGTGTAAAGAAAATTAGACTTACAGGTGGAGAACCATTACTTCGA
AAAGATTTACCGAAGCTGATTAAGCGTCTAGCAAGTCTGGATGGTATAACGGATATTGGACTAACAACAAATGGTATACA
TTTGACAAAACAAGCAAAAGCATTAAAGGAAGCAGGCTTGCAGCGAGTAAACGTTAGTCTGGATGCGATAGAAGATAATG
TATTTCAGAAAATAAATGGACGGAATGTTAGTACGAAACCTGTGTTAAAAGGGATTATAGCTGCTAAAGAGGTCGGTCTT
GAAGTAAAGGTAAATATGGTTGTGAAAAAAGGAATGAATGATAGTCAAGTTCTTCCAATGGCTACTTGTTTTAAAGAACA
GGGAATACCACTTCGTTTTATTGAATTTATGGATGTTGGGAGTACGAACGGATGGAATTTCGAACAGGTAGTTACAAAAC
AAGAATTAATTGAGATGATTGGCAATGTATATCCGATTGAACCCGCTGAACCACACTATTTCGGAGAGGTCGCAAAGCGC
TATTGTTACATAGGAAGTGATGTGGAAGTTGGATTTATCACTTCTGTTTCTGAATCATTTTGTTCTTCATGTACAAGAGC
GCGAATTTCAGCTGATGGAAAGTTTTATACGTGTTTGTTTGCGACAGAAGGTACGGATTTAAGAGCGTTGCTTAGAACCA
ATTGTTCTGATAATGAGTTATTACAAGTCATACAAGATGTATGGAGATATCGAAAAGACCGATATTCTGATGAGCGGACG
GAAGAAAGCGCGAAAAATCGTGCGAAAATTGAAATGTCTTATATTGGAGGATAA

Upstream 100 bases:

>100_bases
TGGAAAGCAAACAAAGAACATGAGCTAAAACATCACAGTTCAAAAATATATTATAATAGAATGAAGAAAGATATGAATGA
AGCAAAAAGGAGGGGTGACG

Downstream 100 bases:

>100_bases
GAAGGGAGGGAAAACATTGCAGGACCGATATTCAAGGCAAATGTTATTTTCAAGTATTGGTGAAGATGGACAGAGGAAAA
TAAGAGAAAAGCATGTATTG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MHEKVRDSFGRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEEFLLTFDEIERLAKLFAGIGVKKIRLTGGEPLLR
KDLPKLIKRLASLDGITDIGLTTNGIHLTKQAKALKEAGLQRVNVSLDAIEDNVFQKINGRNVSTKPVLKGIIAAKEVGL
EVKVNMVVKKGMNDSQVLPMATCFKEQGIPLRFIEFMDVGSTNGWNFEQVVTKQELIEMIGNVYPIEPAEPHYFGEVAKR
YCYIGSDVEVGFITSVSESFCSSCTRARISADGKFYTCLFATEGTDLRALLRTNCSDNELLQVIQDVWRYRKDRYSDERT
EESAKNRAKIEMSYIGG

Sequences:

>Translated_337_residues
MHEKVRDSFGRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEEFLLTFDEIERLAKLFAGIGVKKIRLTGGEPLLR
KDLPKLIKRLASLDGITDIGLTTNGIHLTKQAKALKEAGLQRVNVSLDAIEDNVFQKINGRNVSTKPVLKGIIAAKEVGL
EVKVNMVVKKGMNDSQVLPMATCFKEQGIPLRFIEFMDVGSTNGWNFEQVVTKQELIEMIGNVYPIEPAEPHYFGEVAKR
YCYIGSDVEVGFITSVSESFCSSCTRARISADGKFYTCLFATEGTDLRALLRTNCSDNELLQVIQDVWRYRKDRYSDERT
EESAKNRAKIEMSYIGG
>Mature_337_residues
MHEKVRDSFGRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEEFLLTFDEIERLAKLFAGIGVKKIRLTGGEPLLR
KDLPKLIKRLASLDGITDIGLTTNGIHLTKQAKALKEAGLQRVNVSLDAIEDNVFQKINGRNVSTKPVLKGIIAAKEVGL
EVKVNMVVKKGMNDSQVLPMATCFKEQGIPLRFIEFMDVGSTNGWNFEQVVTKQELIEMIGNVYPIEPAEPHYFGEVAKR
YCYIGSDVEVGFITSVSESFCSSCTRARISADGKFYTCLFATEGTDLRALLRTNCSDNELLQVIQDVWRYRKDRYSDERT
EESAKNRAKIEMSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=334, Percent_Identity=39.8203592814371, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI22027536, Length=334, Percent_Identity=39.8203592814371, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1786999, Length=340, Percent_Identity=34.4117647058824, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI32566331, Length=326, Percent_Identity=36.8098159509202, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI32566329, Length=334, Percent_Identity=36.2275449101796, Blast_Score=197, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17561210, Length=332, Percent_Identity=36.144578313253, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=36.8589743589744, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28574488, Length=332, Percent_Identity=36.144578313253, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_BACCN (A7GU01)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001376604.1
- ProteinModelPortal:   A7GU01
- SMR:   A7GU01
- STRING:   A7GU01
- EnsemblBacteria:   EBBACT00000037300
- GeneID:   5346232
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_3397
- eggNOG:   COG2896
- GeneTree:   EBGT00050000002384
- HOGENOM:   HBG529885
- OMA:   PMARHFR
- ProtClustDB:   CLSK2502962
- BioCyc:   BCER315749:BCER98_3397-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37998; Mature: 37998

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 17-17 BINDING 30-30 BINDING 71-71 BINDING 75-75 BINDING 102-102 BINDING 126-126 BINDING 163-163 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKVRDSFGRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEEFLLTFDEIERLAK
CCCHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHH
LFAGIGVKKIRLTGGEPLLRKDLPKLIKRLASLDGITDIGLTTNGIHLTKQAKALKEAGL
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHCCH
QRVNVSLDAIEDNVFQKINGRNVSTKPVLKGIIAAKEVGLEVKVNMVVKKGMNDSQVLPM
HEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEHEECCCCCCCCCHH
ATCFKEQGIPLRFIEFMDVGSTNGWNFEQVVTKQELIEMIGNVYPIEPAEPHYFGEVAKR
HHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHH
YCYIGSDVEVGFITSVSESFCSSCTRARISADGKFYTCLFATEGTDLRALLRTNCSDNEL
HEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHCCCCHHHH
LQVIQDVWRYRKDRYSDERTEESAKNRAKIEMSYIGG
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCC
>Mature Secondary Structure
MHEKVRDSFGRPLQDLRISVIDRCNFRCTYCMPAEVFGPDYAFLQEEFLLTFDEIERLAK
CCCHHHHHHCCCHHHHHHHHHHCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHH
LFAGIGVKKIRLTGGEPLLRKDLPKLIKRLASLDGITDIGLTTNGIHLTKQAKALKEAGL
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHCCH
QRVNVSLDAIEDNVFQKINGRNVSTKPVLKGIIAAKEVGLEVKVNMVVKKGMNDSQVLPM
HEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEHEECCCCCCCCCHH
ATCFKEQGIPLRFIEFMDVGSTNGWNFEQVVTKQELIEMIGNVYPIEPAEPHYFGEVAKR
HHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHH
YCYIGSDVEVGFITSVSESFCSSCTRARISADGKFYTCLFATEGTDLRALLRTNCSDNEL
HEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHCCCCHHHH
LQVIQDVWRYRKDRYSDERTEESAKNRAKIEMSYIGG
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA